Limits...
PCR diagnostics and monitoring of adenoviral infections in hematopoietic stem cell transplantation recipients.

Bil-Lula I, Ussowicz M, Rybka B, Wendycz-Domalewska D, Ryczan R, Gorczyńska E, Kałwak K, Woźniak M - Arch. Virol. (2010)

Bottom Line: AdV DNA was detected in 52 (44.8%) patients, with mortality reaching 19% in this subgroup.An increased seasonal occurrence of adenoviral infections was observed in autumn and winter.This study also showed a strong interaction between co-infections of AdV and BK polyomavirus in patients undergoing hematopoietic stem cell transplantations.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Chemistry, University of Medicine in Wrocław, ul. Pasteura 2, 50-367 Wrocław, Poland. iwonabil@anmed.am.wroc.pl

ABSTRACT
After stem cell transplantation, human patients are prone to life-threatening opportunistic infections with a plethora of microorganisms. We report a retrospective study on 116 patients (98 children, 18 adults) who were transplanted in a pediatric bone marrow transplantation unit. Blood, urine and stool samples were collected and monitored for adenovirus (AdV) DNA using polymerase chain reaction (PCR) and real-time PCR (RT-PCR) on a regular basis. AdV DNA was detected in 52 (44.8%) patients, with mortality reaching 19% in this subgroup. Variables associated with adenovirus infection were transplantations from matched unrelated donors and older age of the recipient. An increased seasonal occurrence of adenoviral infections was observed in autumn and winter. Analysis of immune reconstitution showed a higher incidence of AdV infections during periods of low T-lymphocyte count. This study also showed a strong interaction between co-infections of AdV and BK polyomavirus in patients undergoing hematopoietic stem cell transplantations.

Show MeSH

Related in: MedlinePlus

Correlation between CD3+ cells (μl) and AdV viral load (copies/μl). CD3+ cell count and AdV copy number were determined on the same day of the post-transplantation period. Viral load refers to blood, plasma, urine and stool samples
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2982951&req=5

Fig4: Correlation between CD3+ cells (μl) and AdV viral load (copies/μl). CD3+ cell count and AdV copy number were determined on the same day of the post-transplantation period. Viral load refers to blood, plasma, urine and stool samples

Mentions: Analysis by median comparison showed that the median number of CD3+ cells in AdV-infected patients (median 490/μl, range 0–3,309/μl) was lower than among AdV-free recipients (median 665/μl, range 0–6,476/μl), p = 0.04. Correspondence analysis confirmed that AdV-free samples correlated with higher CD3+ cells counts (1,000–6,400/μl) (Fig. 4).Fig. 4


PCR diagnostics and monitoring of adenoviral infections in hematopoietic stem cell transplantation recipients.

Bil-Lula I, Ussowicz M, Rybka B, Wendycz-Domalewska D, Ryczan R, Gorczyńska E, Kałwak K, Woźniak M - Arch. Virol. (2010)

Correlation between CD3+ cells (μl) and AdV viral load (copies/μl). CD3+ cell count and AdV copy number were determined on the same day of the post-transplantation period. Viral load refers to blood, plasma, urine and stool samples
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2982951&req=5

Fig4: Correlation between CD3+ cells (μl) and AdV viral load (copies/μl). CD3+ cell count and AdV copy number were determined on the same day of the post-transplantation period. Viral load refers to blood, plasma, urine and stool samples
Mentions: Analysis by median comparison showed that the median number of CD3+ cells in AdV-infected patients (median 490/μl, range 0–3,309/μl) was lower than among AdV-free recipients (median 665/μl, range 0–6,476/μl), p = 0.04. Correspondence analysis confirmed that AdV-free samples correlated with higher CD3+ cells counts (1,000–6,400/μl) (Fig. 4).Fig. 4

Bottom Line: AdV DNA was detected in 52 (44.8%) patients, with mortality reaching 19% in this subgroup.An increased seasonal occurrence of adenoviral infections was observed in autumn and winter.This study also showed a strong interaction between co-infections of AdV and BK polyomavirus in patients undergoing hematopoietic stem cell transplantations.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Chemistry, University of Medicine in Wrocław, ul. Pasteura 2, 50-367 Wrocław, Poland. iwonabil@anmed.am.wroc.pl

ABSTRACT
After stem cell transplantation, human patients are prone to life-threatening opportunistic infections with a plethora of microorganisms. We report a retrospective study on 116 patients (98 children, 18 adults) who were transplanted in a pediatric bone marrow transplantation unit. Blood, urine and stool samples were collected and monitored for adenovirus (AdV) DNA using polymerase chain reaction (PCR) and real-time PCR (RT-PCR) on a regular basis. AdV DNA was detected in 52 (44.8%) patients, with mortality reaching 19% in this subgroup. Variables associated with adenovirus infection were transplantations from matched unrelated donors and older age of the recipient. An increased seasonal occurrence of adenoviral infections was observed in autumn and winter. Analysis of immune reconstitution showed a higher incidence of AdV infections during periods of low T-lymphocyte count. This study also showed a strong interaction between co-infections of AdV and BK polyomavirus in patients undergoing hematopoietic stem cell transplantations.

Show MeSH
Related in: MedlinePlus