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Extraction and Application of Laccases from Shimeji Mushrooms (Pleurotus ostreatus) Residues in Decolourisation of Reactive Dyes and a Comparative Study Using Commercial Laccase from Aspergillus oryzae.

Teixeira RS, Pereira PM, Ferreira-Leitão VS - Enzyme Res (2010)

Bottom Line: Oxidases are able to degrade organic pollutants; however, high costs associated with biocatalysts production still hinder their use in environmental biocatalysis.Our study compared the action of a commercial laccase from Aspergillus oryzae and a rich extract from Pleurotus ostreatus cultivation residues in decolourisation of reactive dyes: Drimaren Blue X-3LR (DMBLR), Drimaren Blue X-BLN (DMBBLN), Drimaren Rubinol X-3LR (DMR), and Drimaren Blue C-R (RBBR).The decolourisation of DMBBLN by commercial laccase showed levels near 60% while the crude extract presented 80% in 24 h.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Technology, Ministry of Science and Technology, Avenue Venezuela, 82 Centro, 20081-312 Rio de Janeiro, RJ, Brazil.

ABSTRACT
Oxidases are able to degrade organic pollutants; however, high costs associated with biocatalysts production still hinder their use in environmental biocatalysis. Our study compared the action of a commercial laccase from Aspergillus oryzae and a rich extract from Pleurotus ostreatus cultivation residues in decolourisation of reactive dyes: Drimaren Blue X-3LR (DMBLR), Drimaren Blue X-BLN (DMBBLN), Drimaren Rubinol X-3LR (DMR), and Drimaren Blue C-R (RBBR). The colour removal was evaluated by considering dye concentration, reaction time, absence or presence of the mediator ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and the source of laccase. The presence of ABTS was essential for decolourisation of DMR (80-90%, 1 h) and RBBR (80-90%, 24 h) with both laccases. The use of ABTS was not necessary in reactions containing DMBLR (85-97%, 1 h) and DMBBLN (63-84%, 24 h). The decolourisation of DMBBLN by commercial laccase showed levels near 60% while the crude extract presented 80% in 24 h.

No MeSH data available.


Related in: MedlinePlus

Absorption spectra of the mixture of the four dyes studied DMBLR, DMBBLN, DMR, and RBBR (60 ppm) before and after decolourisation reaction with commercial laccase in the presence (EM) and absence (E) of ABTS mediator.
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fig3: Absorption spectra of the mixture of the four dyes studied DMBLR, DMBBLN, DMR, and RBBR (60 ppm) before and after decolourisation reaction with commercial laccase in the presence (EM) and absence (E) of ABTS mediator.

Mentions: In general, effluents from textile industries contain a mixture of dyes and auxiliary substances used during the dyeing process. The decolourisation of a mixture containing the four textile dyes was also evaluated. The absorption spectrum of the reaction mixtures containing 60 ppm of each dye, with the laccase rich extract in the absence (E) or presence of ABTS (EM), and with the commercial laccase in the absence (E) or presence of ABTS (EM), was presented in Figures 2 and 3, respectively.


Extraction and Application of Laccases from Shimeji Mushrooms (Pleurotus ostreatus) Residues in Decolourisation of Reactive Dyes and a Comparative Study Using Commercial Laccase from Aspergillus oryzae.

Teixeira RS, Pereira PM, Ferreira-Leitão VS - Enzyme Res (2010)

Absorption spectra of the mixture of the four dyes studied DMBLR, DMBBLN, DMR, and RBBR (60 ppm) before and after decolourisation reaction with commercial laccase in the presence (EM) and absence (E) of ABTS mediator.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2968717&req=5

fig3: Absorption spectra of the mixture of the four dyes studied DMBLR, DMBBLN, DMR, and RBBR (60 ppm) before and after decolourisation reaction with commercial laccase in the presence (EM) and absence (E) of ABTS mediator.
Mentions: In general, effluents from textile industries contain a mixture of dyes and auxiliary substances used during the dyeing process. The decolourisation of a mixture containing the four textile dyes was also evaluated. The absorption spectrum of the reaction mixtures containing 60 ppm of each dye, with the laccase rich extract in the absence (E) or presence of ABTS (EM), and with the commercial laccase in the absence (E) or presence of ABTS (EM), was presented in Figures 2 and 3, respectively.

Bottom Line: Oxidases are able to degrade organic pollutants; however, high costs associated with biocatalysts production still hinder their use in environmental biocatalysis.Our study compared the action of a commercial laccase from Aspergillus oryzae and a rich extract from Pleurotus ostreatus cultivation residues in decolourisation of reactive dyes: Drimaren Blue X-3LR (DMBLR), Drimaren Blue X-BLN (DMBBLN), Drimaren Rubinol X-3LR (DMR), and Drimaren Blue C-R (RBBR).The decolourisation of DMBBLN by commercial laccase showed levels near 60% while the crude extract presented 80% in 24 h.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Technology, Ministry of Science and Technology, Avenue Venezuela, 82 Centro, 20081-312 Rio de Janeiro, RJ, Brazil.

ABSTRACT
Oxidases are able to degrade organic pollutants; however, high costs associated with biocatalysts production still hinder their use in environmental biocatalysis. Our study compared the action of a commercial laccase from Aspergillus oryzae and a rich extract from Pleurotus ostreatus cultivation residues in decolourisation of reactive dyes: Drimaren Blue X-3LR (DMBLR), Drimaren Blue X-BLN (DMBBLN), Drimaren Rubinol X-3LR (DMR), and Drimaren Blue C-R (RBBR). The colour removal was evaluated by considering dye concentration, reaction time, absence or presence of the mediator ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and the source of laccase. The presence of ABTS was essential for decolourisation of DMR (80-90%, 1 h) and RBBR (80-90%, 24 h) with both laccases. The use of ABTS was not necessary in reactions containing DMBLR (85-97%, 1 h) and DMBBLN (63-84%, 24 h). The decolourisation of DMBBLN by commercial laccase showed levels near 60% while the crude extract presented 80% in 24 h.

No MeSH data available.


Related in: MedlinePlus