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Behçet's disease (Adamantiades-Behçet's disease).

Kaneko F, Togashi A, Saito S, Sakuma H, Oyama N, Nakamura K, Yokota K, Oguma K - Clin. Dev. Immunol. (2010)

Bottom Line: Adamantiades-Behçet's disease (ABD) is characterized by starting with oral aphthous ulceration and developing of the systemic involvements.Although Hsp-65/60 has homologies with the respective T cell epitope, it stimulates peripheral blood mononuclear cells (PBMCs) from ABD patients.On the other hand, some peptides of Hsp-65 were found to reduce IL-8 and IL-12 production from PBMCs of ABD patients in active stage.

View Article: PubMed Central - PubMed

Affiliation: Institute of Dermato-Immunology and Allergy, Southern TOHOKU Research Institute for Neuroscience, 7-115 Yatsuyamada, Koriyama, Fukushima 963-8563, Japan. f.kaneko@mt.strins.or.jp

ABSTRACT
Adamantiades-Behçet's disease (ABD) is characterized by starting with oral aphthous ulceration and developing of the systemic involvements. The pathogenesis of ABD is closely correlated with the genetic factors and the triggering factors which acquire delayed-type hypersensitivity reaction against oral streptococci mediated by IL-12 cytokine family. HLA-B51 is associated in more than 60% of the patients and its restricted CD8+ T cell response is clearly correlated with the target tissues. Bes-1 gene encoded partial S. sanguinis genome which is highly homologous with retinal protein, and 65 kD heat shock protein (Hsp-65) released from streptococci is playing an important role with human Hsp-60 in the pathogenesis of ABD. Although Hsp-65/60 has homologies with the respective T cell epitope, it stimulates peripheral blood mononuclear cells (PBMCs) from ABD patients. On the other hand, some peptides of Hsp-65 were found to reduce IL-8 and IL-12 production from PBMCs of ABD patients in active stage.

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Streptococcal colonies appeared from saliva of an ABD patient in MS agar dish.  According to the instruction of the MS agar (Difco Lab.), mainly 3 kinds of bacterial colonies are differentiated as blue crystal (probably S. mitis, etc.), gum-drop (S. salivarius, etc.), and dip (micrococcus species, etc.) shapes at 3–5 day culture.
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fig1: Streptococcal colonies appeared from saliva of an ABD patient in MS agar dish. According to the instruction of the MS agar (Difco Lab.), mainly 3 kinds of bacterial colonies are differentiated as blue crystal (probably S. mitis, etc.), gum-drop (S. salivarius, etc.), and dip (micrococcus species, etc.) shapes at 3–5 day culture.

Mentions: The oral streptococci can be generally observed as main 3 kinds of streptococcal colonies appearing at 3–5 day incubation of the saliva in MS (Mitis-Salivarius) agar with 1% tellunite solution dish which streptococci are selectively grown (Difco Lab., Detroit, USA) (Figure 1). Forty-eight hours after S-prick, the DTH reaction appeared at the prick site in more than 70% of probability in ABD patients (Figure 2(a)) [19]. Since the skin reaction did not appear by the sterilized saliva (SS-prick) using the syringe filter with 0.2 μm (Nalgene, Nunc International Co., USA), the S-prick reaction is considered to be due to oral streptococci. The histology of the biopsy specimen from the S-prick reaction shows edema in the upper dermis and mononuclear cell infiltration around and in the walls of the vessels in the dermis (Figure 2(b)). The infiltrated mononuclear cells were mainly composed of CD3+/CD4+ cells > CD68+ cells (Figure 2(c)) >CD8+ cells and no CD20+ and CD56+ cells were found, suggesting DTH reaction. This histologic findings seem to be similar to that of the pathergy reaction exhibiting vascular changes [21] and also similar to that of EN-like eruption appeared on the lower leg of ABD patients [12, 16, 17]. Generally, in EN-like eruption, mononuclear cells were infiltrated around vessels in the middle dermis of the biopsy specimen which was taken at 5th day after the appearance and the infiltrated cells were mainly composed of CD3+/CD4+ cells >CD8+ cells > CD68+ cells. These results provide evidence that S-prick is highly diagnostic for ABD patients and also suggest that the pathergy reaction might be due to cutaneous bacteria having cross immunity to oral streptococci.


Behçet's disease (Adamantiades-Behçet's disease).

Kaneko F, Togashi A, Saito S, Sakuma H, Oyama N, Nakamura K, Yokota K, Oguma K - Clin. Dev. Immunol. (2010)

Streptococcal colonies appeared from saliva of an ABD patient in MS agar dish.  According to the instruction of the MS agar (Difco Lab.), mainly 3 kinds of bacterial colonies are differentiated as blue crystal (probably S. mitis, etc.), gum-drop (S. salivarius, etc.), and dip (micrococcus species, etc.) shapes at 3–5 day culture.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2967828&req=5

fig1: Streptococcal colonies appeared from saliva of an ABD patient in MS agar dish. According to the instruction of the MS agar (Difco Lab.), mainly 3 kinds of bacterial colonies are differentiated as blue crystal (probably S. mitis, etc.), gum-drop (S. salivarius, etc.), and dip (micrococcus species, etc.) shapes at 3–5 day culture.
Mentions: The oral streptococci can be generally observed as main 3 kinds of streptococcal colonies appearing at 3–5 day incubation of the saliva in MS (Mitis-Salivarius) agar with 1% tellunite solution dish which streptococci are selectively grown (Difco Lab., Detroit, USA) (Figure 1). Forty-eight hours after S-prick, the DTH reaction appeared at the prick site in more than 70% of probability in ABD patients (Figure 2(a)) [19]. Since the skin reaction did not appear by the sterilized saliva (SS-prick) using the syringe filter with 0.2 μm (Nalgene, Nunc International Co., USA), the S-prick reaction is considered to be due to oral streptococci. The histology of the biopsy specimen from the S-prick reaction shows edema in the upper dermis and mononuclear cell infiltration around and in the walls of the vessels in the dermis (Figure 2(b)). The infiltrated mononuclear cells were mainly composed of CD3+/CD4+ cells > CD68+ cells (Figure 2(c)) >CD8+ cells and no CD20+ and CD56+ cells were found, suggesting DTH reaction. This histologic findings seem to be similar to that of the pathergy reaction exhibiting vascular changes [21] and also similar to that of EN-like eruption appeared on the lower leg of ABD patients [12, 16, 17]. Generally, in EN-like eruption, mononuclear cells were infiltrated around vessels in the middle dermis of the biopsy specimen which was taken at 5th day after the appearance and the infiltrated cells were mainly composed of CD3+/CD4+ cells >CD8+ cells > CD68+ cells. These results provide evidence that S-prick is highly diagnostic for ABD patients and also suggest that the pathergy reaction might be due to cutaneous bacteria having cross immunity to oral streptococci.

Bottom Line: Adamantiades-Behçet's disease (ABD) is characterized by starting with oral aphthous ulceration and developing of the systemic involvements.Although Hsp-65/60 has homologies with the respective T cell epitope, it stimulates peripheral blood mononuclear cells (PBMCs) from ABD patients.On the other hand, some peptides of Hsp-65 were found to reduce IL-8 and IL-12 production from PBMCs of ABD patients in active stage.

View Article: PubMed Central - PubMed

Affiliation: Institute of Dermato-Immunology and Allergy, Southern TOHOKU Research Institute for Neuroscience, 7-115 Yatsuyamada, Koriyama, Fukushima 963-8563, Japan. f.kaneko@mt.strins.or.jp

ABSTRACT
Adamantiades-Behçet's disease (ABD) is characterized by starting with oral aphthous ulceration and developing of the systemic involvements. The pathogenesis of ABD is closely correlated with the genetic factors and the triggering factors which acquire delayed-type hypersensitivity reaction against oral streptococci mediated by IL-12 cytokine family. HLA-B51 is associated in more than 60% of the patients and its restricted CD8+ T cell response is clearly correlated with the target tissues. Bes-1 gene encoded partial S. sanguinis genome which is highly homologous with retinal protein, and 65 kD heat shock protein (Hsp-65) released from streptococci is playing an important role with human Hsp-60 in the pathogenesis of ABD. Although Hsp-65/60 has homologies with the respective T cell epitope, it stimulates peripheral blood mononuclear cells (PBMCs) from ABD patients. On the other hand, some peptides of Hsp-65 were found to reduce IL-8 and IL-12 production from PBMCs of ABD patients in active stage.

Show MeSH
Related in: MedlinePlus