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Study of Soybean Oil Hydrolysis Catalyzed by Thermomyces lanuginosus Lipase and Its Application to Biodiesel Production via Hydroesterification.

Cavalcanti-Oliveira Ed, da Silva PR, Ramos AP, Aranda DA, Freire DM - Enzyme Res (2010)

Bottom Line: The process of biodiesel production by the hydroesterification route that is proposed here involves a first step consisting of triacylglyceride hydrolysis catalyzed by lipase from Thermomyces lanuginosus (TL 100L) to generate free fatty acids (FFAs).The best result for the enzyme-catalyzed hydrolysis was obtained under reaction conditions of 50% (v/v) soybean oil and 2.3% (v/v) lipase (25 U/mL of reaction medium) in distilled water and at 60°C; an 89% conversion rate to FFAs was obtained after 48 hours of reaction.This study is exceptional because both the hydrolysis and the esterification use a simple reaction medium with high substrate concentrations.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biotecnologia Microbiana (LaBiM), Centro de Tecnologia, Instituto de Química, lab. 549-1, Universidade Federal do Rio de Janeiro, CEP 21945-970, Rio de Janeiro, RJ, Brazil.

ABSTRACT
The process of biodiesel production by the hydroesterification route that is proposed here involves a first step consisting of triacylglyceride hydrolysis catalyzed by lipase from Thermomyces lanuginosus (TL 100L) to generate free fatty acids (FFAs). This step is followed by esterification of the FFAs with alcohol, catalyzed by niobic acid in pellets or without a catalyst. The best result for the enzyme-catalyzed hydrolysis was obtained under reaction conditions of 50% (v/v) soybean oil and 2.3% (v/v) lipase (25 U/mL of reaction medium) in distilled water and at 60°C; an 89% conversion rate to FFAs was obtained after 48 hours of reaction. For the esterification reaction, the best result was with an FFA/methanol molar ratio of 1:3, niobic acid catalyst at a concentration of 20% (w/w FFA), and 200°C, which yielded 92% conversion of FFAs to soy methyl esters after 1 hour of reaction. This study is exceptional because both the hydrolysis and the esterification use a simple reaction medium with high substrate concentrations.

No MeSH data available.


Related in: MedlinePlus

Contour plot for FFAs concentration as a function of lipase content and temperature, at an oil concentration of 62%.
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Related In: Results  -  Collection


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fig3: Contour plot for FFAs concentration as a function of lipase content and temperature, at an oil concentration of 62%.

Mentions: Figures 3, 4, and 5 show the contour plots of the concentrations of FFAs obtained by the model of (1).


Study of Soybean Oil Hydrolysis Catalyzed by Thermomyces lanuginosus Lipase and Its Application to Biodiesel Production via Hydroesterification.

Cavalcanti-Oliveira Ed, da Silva PR, Ramos AP, Aranda DA, Freire DM - Enzyme Res (2010)

Contour plot for FFAs concentration as a function of lipase content and temperature, at an oil concentration of 62%.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2967827&req=5

fig3: Contour plot for FFAs concentration as a function of lipase content and temperature, at an oil concentration of 62%.
Mentions: Figures 3, 4, and 5 show the contour plots of the concentrations of FFAs obtained by the model of (1).

Bottom Line: The process of biodiesel production by the hydroesterification route that is proposed here involves a first step consisting of triacylglyceride hydrolysis catalyzed by lipase from Thermomyces lanuginosus (TL 100L) to generate free fatty acids (FFAs).The best result for the enzyme-catalyzed hydrolysis was obtained under reaction conditions of 50% (v/v) soybean oil and 2.3% (v/v) lipase (25 U/mL of reaction medium) in distilled water and at 60°C; an 89% conversion rate to FFAs was obtained after 48 hours of reaction.This study is exceptional because both the hydrolysis and the esterification use a simple reaction medium with high substrate concentrations.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biotecnologia Microbiana (LaBiM), Centro de Tecnologia, Instituto de Química, lab. 549-1, Universidade Federal do Rio de Janeiro, CEP 21945-970, Rio de Janeiro, RJ, Brazil.

ABSTRACT
The process of biodiesel production by the hydroesterification route that is proposed here involves a first step consisting of triacylglyceride hydrolysis catalyzed by lipase from Thermomyces lanuginosus (TL 100L) to generate free fatty acids (FFAs). This step is followed by esterification of the FFAs with alcohol, catalyzed by niobic acid in pellets or without a catalyst. The best result for the enzyme-catalyzed hydrolysis was obtained under reaction conditions of 50% (v/v) soybean oil and 2.3% (v/v) lipase (25 U/mL of reaction medium) in distilled water and at 60°C; an 89% conversion rate to FFAs was obtained after 48 hours of reaction. For the esterification reaction, the best result was with an FFA/methanol molar ratio of 1:3, niobic acid catalyst at a concentration of 20% (w/w FFA), and 200°C, which yielded 92% conversion of FFAs to soy methyl esters after 1 hour of reaction. This study is exceptional because both the hydrolysis and the esterification use a simple reaction medium with high substrate concentrations.

No MeSH data available.


Related in: MedlinePlus