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Biological effects of a porcine-derived collagen membrane on intrabony defects.

Lee CK, Koo KT, Kim TI, Seol YJ, Lee YM, Rhyu IC, Ku Y, Chung CP, Park YJ, Lee JY - J Periodontal Implant Sci (2010)

Bottom Line: For all parameters evaluating bone regeneration, the experimental group showed significantly superior results compared to the control.Also, in new bone area (NBa) and new bone volume (NBv), the experimental group showed superior results compared to the control (NBa, 34.48 ± 10.21% vs. 5.09 ± 5.76%, P = 0.014; and NBv, 28.04 ± 12.96 vs. 1.55 ± 0.57, P = 0.041).On the other hand, for parameters evaluating periodontal tissue regeneration, including junctional epithelium migration and new cementum height, there were no statistically significant differences between two groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: To prolong the degradation time of collagen membranes, various cross-linking techniques have been developed. For cross-linking, chemicals such as formaldehyde and glutaraldehyde are added to collagen membranes, but these chemicals could adversely affect surrounding tissues. The aim of this study is to evaluate the ability of porous non-chemical cross-linking porcine-derived collagen nanofibrous membrane to enhance bone and associated tissue regeneration in one-wall intrabony defects in beagle dogs.

Methods: The second and third mandibular premolars and the first molars of 2 adult beagles were extracted bilaterally and the extraction sites were allowed to heal for 10 weeks. One-wall intrabony defects were prepared bilaterally on the mesial and distal side of the fourth mandibular premolars. Among eight defects, four defects were not covered with membrane as controls and the other four defects were covered with membrane as the experimental group. The animals were sacrificed 10 weeks after surgery.

Results: Wound healing was generally uneventful. For all parameters evaluating bone regeneration, the experimental group showed significantly superior results compared to the control. In new bone height (NBh), the experimental group exhibited a greater mean value than the control (3.04 ± 0.23 mm/1.57 ± 0.59, P = 0.003). Also, in new bone area (NBa) and new bone volume (NBv), the experimental group showed superior results compared to the control (NBa, 34.48 ± 10.21% vs. 5.09 ± 5.76%, P = 0.014; and NBv, 28.04 ± 12.96 vs. 1.55 ± 0.57, P = 0.041). On the other hand, for parameters evaluating periodontal tissue regeneration, including junctional epithelium migration and new cementum height, there were no statistically significant differences between two groups.

Conclusions: Within the limitations of this study, this collagen membrane enhanced bone regeneration at one-wall intrabony defects. On the other hand, no influence of this membrane on periodontal tissue regeneration could be ascertained in this study.

No MeSH data available.


Clinical photography representing the surgical procedure. (A) 4 (mesio-distal) × 5 (apico-coronal) mm one-wall intrabony defects were prepared at the mesial and distal portion of the fourth premolar. (B) One defect was not covered with membrane (control), and the other was covered with membrane (experimental). The membrane was stabilized with a fixation screw (arrowheads: border of collagen membrane). (C) Primary flap closure was achieved at all defect sites.
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Figure 1: Clinical photography representing the surgical procedure. (A) 4 (mesio-distal) × 5 (apico-coronal) mm one-wall intrabony defects were prepared at the mesial and distal portion of the fourth premolar. (B) One defect was not covered with membrane (control), and the other was covered with membrane (experimental). The membrane was stabilized with a fixation screw (arrowheads: border of collagen membrane). (C) Primary flap closure was achieved at all defect sites.

Mentions: After the 10-week healing interval, one-wall intrabony defects were prepared bilaterally on the mesial and distal side of the fourth mandibular premolars. Crestal incisions of 20 mm length were made on the mesial and distal side of the fourth premolars and connected with sulcular incisions made circumferentially. Vertical incisions were made at the end of the crestal incision, followed by periodontal flap reflections. After flap reflection, one-wall intrabony defects sized 4 × 5 mm (mesio-distal width and apico-coronal height, respectively) were created on the mesial and distal sides of the fourth premolars using round and fissure burs with sterile saline irrigation (Fig. 1A). Cementum was removed completely by root planing using Gracey curettes and chisels. Reference notches were made with a #1 round bur on the root surface at the base of the defect, and at the cementoenamel junction (CEJ) for histometric analysis.


Biological effects of a porcine-derived collagen membrane on intrabony defects.

Lee CK, Koo KT, Kim TI, Seol YJ, Lee YM, Rhyu IC, Ku Y, Chung CP, Park YJ, Lee JY - J Periodontal Implant Sci (2010)

Clinical photography representing the surgical procedure. (A) 4 (mesio-distal) × 5 (apico-coronal) mm one-wall intrabony defects were prepared at the mesial and distal portion of the fourth premolar. (B) One defect was not covered with membrane (control), and the other was covered with membrane (experimental). The membrane was stabilized with a fixation screw (arrowheads: border of collagen membrane). (C) Primary flap closure was achieved at all defect sites.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2967811&req=5

Figure 1: Clinical photography representing the surgical procedure. (A) 4 (mesio-distal) × 5 (apico-coronal) mm one-wall intrabony defects were prepared at the mesial and distal portion of the fourth premolar. (B) One defect was not covered with membrane (control), and the other was covered with membrane (experimental). The membrane was stabilized with a fixation screw (arrowheads: border of collagen membrane). (C) Primary flap closure was achieved at all defect sites.
Mentions: After the 10-week healing interval, one-wall intrabony defects were prepared bilaterally on the mesial and distal side of the fourth mandibular premolars. Crestal incisions of 20 mm length were made on the mesial and distal side of the fourth premolars and connected with sulcular incisions made circumferentially. Vertical incisions were made at the end of the crestal incision, followed by periodontal flap reflections. After flap reflection, one-wall intrabony defects sized 4 × 5 mm (mesio-distal width and apico-coronal height, respectively) were created on the mesial and distal sides of the fourth premolars using round and fissure burs with sterile saline irrigation (Fig. 1A). Cementum was removed completely by root planing using Gracey curettes and chisels. Reference notches were made with a #1 round bur on the root surface at the base of the defect, and at the cementoenamel junction (CEJ) for histometric analysis.

Bottom Line: For all parameters evaluating bone regeneration, the experimental group showed significantly superior results compared to the control.Also, in new bone area (NBa) and new bone volume (NBv), the experimental group showed superior results compared to the control (NBa, 34.48 ± 10.21% vs. 5.09 ± 5.76%, P = 0.014; and NBv, 28.04 ± 12.96 vs. 1.55 ± 0.57, P = 0.041).On the other hand, for parameters evaluating periodontal tissue regeneration, including junctional epithelium migration and new cementum height, there were no statistically significant differences between two groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: To prolong the degradation time of collagen membranes, various cross-linking techniques have been developed. For cross-linking, chemicals such as formaldehyde and glutaraldehyde are added to collagen membranes, but these chemicals could adversely affect surrounding tissues. The aim of this study is to evaluate the ability of porous non-chemical cross-linking porcine-derived collagen nanofibrous membrane to enhance bone and associated tissue regeneration in one-wall intrabony defects in beagle dogs.

Methods: The second and third mandibular premolars and the first molars of 2 adult beagles were extracted bilaterally and the extraction sites were allowed to heal for 10 weeks. One-wall intrabony defects were prepared bilaterally on the mesial and distal side of the fourth mandibular premolars. Among eight defects, four defects were not covered with membrane as controls and the other four defects were covered with membrane as the experimental group. The animals were sacrificed 10 weeks after surgery.

Results: Wound healing was generally uneventful. For all parameters evaluating bone regeneration, the experimental group showed significantly superior results compared to the control. In new bone height (NBh), the experimental group exhibited a greater mean value than the control (3.04 ± 0.23 mm/1.57 ± 0.59, P = 0.003). Also, in new bone area (NBa) and new bone volume (NBv), the experimental group showed superior results compared to the control (NBa, 34.48 ± 10.21% vs. 5.09 ± 5.76%, P = 0.014; and NBv, 28.04 ± 12.96 vs. 1.55 ± 0.57, P = 0.041). On the other hand, for parameters evaluating periodontal tissue regeneration, including junctional epithelium migration and new cementum height, there were no statistically significant differences between two groups.

Conclusions: Within the limitations of this study, this collagen membrane enhanced bone regeneration at one-wall intrabony defects. On the other hand, no influence of this membrane on periodontal tissue regeneration could be ascertained in this study.

No MeSH data available.