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Effects of fibrin-binding oligopeptide on osteopromotion in rabbit calvarial defects.

Lee JA, Ku Y, Rhyu IC, Chung CP, Park YJ - J Periodontal Implant Sci (2010)

Bottom Line: At 8 weeks, both sites showed increased new bone formation.However, the difference between the two sites had reduced.This result suggests that these oligopeptides can be beneficial in reconstructing oral and maxillofacial deformities or in regenerating osseous bone defects.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: Fibronectin (FN) has been shown to stimulate bone regeneration in animal models. The aim of this study was to evaluate the capacity of bovine bone mineral coated with synthetic oligopeptides to enhance bone regeneration in rabbit calvarial defects.

Methods: Oligopeptides including fibrin-binding sequences of FN repeats were synthesized on the basis of primary and tertiary human plasma FN structures. Peptide coated and uncoated bone minerals were implanted into 10 mm calvarial defects in New Zealand white rabbits, and the animals were sacrificed at 4 or 8 weeks after surgery. After specimens were prepared, histologic examination and histomorphometric analysis were performed.

Results: At 4 weeks after surgery, the uncoated groups showed a limited amount of osteoid formation at the periphery of the defect and the oligopeptide coated groups showed more osteoid formation and new bone formation in the center of the defect as well as at the periphery. At 8 weeks, both sites showed increased new bone formation. However, the difference between the two sites had reduced.

Conclusions: Fibrin-binding synthetic oligopeptide derived from FN on deproteinized bovine bone enhanced new bone formation in rabbit calvarial defects at the early healing stage. This result suggests that these oligopeptides can be beneficial in reconstructing oral and maxillofacial deformities or in regenerating osseous bone defects.

No MeSH data available.


Related in: MedlinePlus

(A) Histologic view of the uncoated bone group after 8 weeks. New bone formation was noted throughout the defect. Arrows indicate the margin of the bone defect. (B) Histologic view of the peptide coated bone group after 8 weeks. New bone formation was obvious, and the defect tended to coalesce with new bone. Arrows indicate the margin of the bone defect (H&E staining).
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Figure 5: (A) Histologic view of the uncoated bone group after 8 weeks. New bone formation was noted throughout the defect. Arrows indicate the margin of the bone defect. (B) Histologic view of the peptide coated bone group after 8 weeks. New bone formation was obvious, and the defect tended to coalesce with new bone. Arrows indicate the margin of the bone defect (H&E staining).

Mentions: At 8 weeks, the amount of the new bone was greater than that observed at 4 weeks in both groups. New bone was deposited evenly around the graft material and extended to the central area of the defects (Fig. 5). The bone mineral was integrated with the new bone and newly formed bones had connected to each other (Fig. 6), especially in the peptide coated groups. In higher magnification, multiple bony islands filled the central part of the defect (Fig. 7A) and connected each other. In the peptide coated group, new bone showed a more mature pattern (Fig. 7B). The particles of DBB were easily identified. Neither resorption lacunae nor active osteoclasts were found in specimens at 4 and 8 weeks.


Effects of fibrin-binding oligopeptide on osteopromotion in rabbit calvarial defects.

Lee JA, Ku Y, Rhyu IC, Chung CP, Park YJ - J Periodontal Implant Sci (2010)

(A) Histologic view of the uncoated bone group after 8 weeks. New bone formation was noted throughout the defect. Arrows indicate the margin of the bone defect. (B) Histologic view of the peptide coated bone group after 8 weeks. New bone formation was obvious, and the defect tended to coalesce with new bone. Arrows indicate the margin of the bone defect (H&E staining).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2967808&req=5

Figure 5: (A) Histologic view of the uncoated bone group after 8 weeks. New bone formation was noted throughout the defect. Arrows indicate the margin of the bone defect. (B) Histologic view of the peptide coated bone group after 8 weeks. New bone formation was obvious, and the defect tended to coalesce with new bone. Arrows indicate the margin of the bone defect (H&E staining).
Mentions: At 8 weeks, the amount of the new bone was greater than that observed at 4 weeks in both groups. New bone was deposited evenly around the graft material and extended to the central area of the defects (Fig. 5). The bone mineral was integrated with the new bone and newly formed bones had connected to each other (Fig. 6), especially in the peptide coated groups. In higher magnification, multiple bony islands filled the central part of the defect (Fig. 7A) and connected each other. In the peptide coated group, new bone showed a more mature pattern (Fig. 7B). The particles of DBB were easily identified. Neither resorption lacunae nor active osteoclasts were found in specimens at 4 and 8 weeks.

Bottom Line: At 8 weeks, both sites showed increased new bone formation.However, the difference between the two sites had reduced.This result suggests that these oligopeptides can be beneficial in reconstructing oral and maxillofacial deformities or in regenerating osseous bone defects.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: Fibronectin (FN) has been shown to stimulate bone regeneration in animal models. The aim of this study was to evaluate the capacity of bovine bone mineral coated with synthetic oligopeptides to enhance bone regeneration in rabbit calvarial defects.

Methods: Oligopeptides including fibrin-binding sequences of FN repeats were synthesized on the basis of primary and tertiary human plasma FN structures. Peptide coated and uncoated bone minerals were implanted into 10 mm calvarial defects in New Zealand white rabbits, and the animals were sacrificed at 4 or 8 weeks after surgery. After specimens were prepared, histologic examination and histomorphometric analysis were performed.

Results: At 4 weeks after surgery, the uncoated groups showed a limited amount of osteoid formation at the periphery of the defect and the oligopeptide coated groups showed more osteoid formation and new bone formation in the center of the defect as well as at the periphery. At 8 weeks, both sites showed increased new bone formation. However, the difference between the two sites had reduced.

Conclusions: Fibrin-binding synthetic oligopeptide derived from FN on deproteinized bovine bone enhanced new bone formation in rabbit calvarial defects at the early healing stage. This result suggests that these oligopeptides can be beneficial in reconstructing oral and maxillofacial deformities or in regenerating osseous bone defects.

No MeSH data available.


Related in: MedlinePlus