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Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China.

Guo LJ, Lu YH, Wei YW, Huang LP, Liu CM - Virol. J. (2010)

Bottom Line: Amino acid sequence analysis identified two novel ORF2 mutations (resulting in ORF2 sequences 705 and 708 nt in length) in three deletion strains (1766 nt) and one strain with a genome 1767 nt in length.The results of this study provide evidence that PCV2 is undergoing constant genetic variation and that the predominant strain in China as well as the antigenic situation has changed in recent years.Furthermore, the PCR-RFLP method presented here may be useful for the differential identification of PCV2 strains in future studies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Nangang District, Harbin, P.R. China.

ABSTRACT

Background: Porcine circovirus type 2 (PCV2), the causative agent of postweaning multisystemic wasting syndrome (PMWS), is a serious economic problem for the swine industry in China. In this study, we investigated the genetic variation of PCV2 in China using strains isolated from 2004-2008. Viruses were isolated from samples collected from pigs with multi-systemic lesions and clinical signs of PMWS from different regions of China, and the genomes of these viruses were sequenced. The assembled sequences were used to define the genotypes of these strains; PCR-RFLP methodology was used to distinguish isolates and capture ELISA was used to demonstrate the antigenic changes resulted from ORF2 gene mutation of the isolates.

Results: We identified 19 PCV2 isolates, including four newly emerging PCV2 mutant strains. The 19 isolates were designated into three genotypes (PCV2a, PCV2b and PCV2d). PCV2d represented a novel genotype and a shift from PCV2a to PCV2b as the predominant genotype in China was identified. This is the first report of 1766 nt PCV2 harboring a base deletion at other new different positions. Amino acid sequence analysis identified two novel ORF2 mutations (resulting in ORF2 sequences 705 and 708 nt in length) in three deletion strains (1766 nt) and one strain with a genome 1767 nt in length. Finding of two amino acids elongation of the ORF2-encoded Cap protein is firstly observed among PCV2 strains all over the world. The isolates were distinguished into different genotypes by PCR-RFLP methodology and antigenic changes were present in Cap protein of mutation isolates by capture ELISA.

Conclusions: The results of this study provide evidence that PCV2 is undergoing constant genetic variation and that the predominant strain in China as well as the antigenic situation has changed in recent years. Furthermore, the PCR-RFLP method presented here may be useful for the differential identification of PCV2 strains in future studies.

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Related in: MedlinePlus

Alignment of the ORF2 gene sequences (702/705/708 bp) encoding the capsid protein in the 19 isolates, using the ClustalW program within the DNA Star 6.0 software. Mutations in the stop codon in four isolates (YJ, AH, MDJ and BDH) resulted in elongation of the ORF by one codon, and a one base deletion in isolate YJ at position 1039 led to a two-codon elongation of the ORF.
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Figure 5: Alignment of the ORF2 gene sequences (702/705/708 bp) encoding the capsid protein in the 19 isolates, using the ClustalW program within the DNA Star 6.0 software. Mutations in the stop codon in four isolates (YJ, AH, MDJ and BDH) resulted in elongation of the ORF by one codon, and a one base deletion in isolate YJ at position 1039 led to a two-codon elongation of the ORF.

Mentions: The 19 ORF2-encoded Cap protein sequences were relatively conserved, although a few mutations existed (Figure 4). Important mutations located in the C terminus of the Cap protein are indicated by the red circle in Figure 4. Strain YJ showed an elongation of two amino acids (Asn and Glu), and strains AH, BDH and MDJ showed an elongation of one amino acid (Lys) compared with the PCV2a isolations. Analysis of the ORF2 gene in the 19 strains indicated a one-base deletion at position 1039 in the genome of strain YJ, resulting in an ORF2 gene of 708 nt. While in strains AH, BDH and MDJ, a stop codon mutation in the ORF2 resulted in an ORF2 gene of 705 nt (Figure 5). ORF2 gene was elongated by one or two codons due to the deletion and mutation mentioned above.


Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China.

Guo LJ, Lu YH, Wei YW, Huang LP, Liu CM - Virol. J. (2010)

Alignment of the ORF2 gene sequences (702/705/708 bp) encoding the capsid protein in the 19 isolates, using the ClustalW program within the DNA Star 6.0 software. Mutations in the stop codon in four isolates (YJ, AH, MDJ and BDH) resulted in elongation of the ORF by one codon, and a one base deletion in isolate YJ at position 1039 led to a two-codon elongation of the ORF.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2967541&req=5

Figure 5: Alignment of the ORF2 gene sequences (702/705/708 bp) encoding the capsid protein in the 19 isolates, using the ClustalW program within the DNA Star 6.0 software. Mutations in the stop codon in four isolates (YJ, AH, MDJ and BDH) resulted in elongation of the ORF by one codon, and a one base deletion in isolate YJ at position 1039 led to a two-codon elongation of the ORF.
Mentions: The 19 ORF2-encoded Cap protein sequences were relatively conserved, although a few mutations existed (Figure 4). Important mutations located in the C terminus of the Cap protein are indicated by the red circle in Figure 4. Strain YJ showed an elongation of two amino acids (Asn and Glu), and strains AH, BDH and MDJ showed an elongation of one amino acid (Lys) compared with the PCV2a isolations. Analysis of the ORF2 gene in the 19 strains indicated a one-base deletion at position 1039 in the genome of strain YJ, resulting in an ORF2 gene of 708 nt. While in strains AH, BDH and MDJ, a stop codon mutation in the ORF2 resulted in an ORF2 gene of 705 nt (Figure 5). ORF2 gene was elongated by one or two codons due to the deletion and mutation mentioned above.

Bottom Line: Amino acid sequence analysis identified two novel ORF2 mutations (resulting in ORF2 sequences 705 and 708 nt in length) in three deletion strains (1766 nt) and one strain with a genome 1767 nt in length.The results of this study provide evidence that PCV2 is undergoing constant genetic variation and that the predominant strain in China as well as the antigenic situation has changed in recent years.Furthermore, the PCR-RFLP method presented here may be useful for the differential identification of PCV2 strains in future studies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Nangang District, Harbin, P.R. China.

ABSTRACT

Background: Porcine circovirus type 2 (PCV2), the causative agent of postweaning multisystemic wasting syndrome (PMWS), is a serious economic problem for the swine industry in China. In this study, we investigated the genetic variation of PCV2 in China using strains isolated from 2004-2008. Viruses were isolated from samples collected from pigs with multi-systemic lesions and clinical signs of PMWS from different regions of China, and the genomes of these viruses were sequenced. The assembled sequences were used to define the genotypes of these strains; PCR-RFLP methodology was used to distinguish isolates and capture ELISA was used to demonstrate the antigenic changes resulted from ORF2 gene mutation of the isolates.

Results: We identified 19 PCV2 isolates, including four newly emerging PCV2 mutant strains. The 19 isolates were designated into three genotypes (PCV2a, PCV2b and PCV2d). PCV2d represented a novel genotype and a shift from PCV2a to PCV2b as the predominant genotype in China was identified. This is the first report of 1766 nt PCV2 harboring a base deletion at other new different positions. Amino acid sequence analysis identified two novel ORF2 mutations (resulting in ORF2 sequences 705 and 708 nt in length) in three deletion strains (1766 nt) and one strain with a genome 1767 nt in length. Finding of two amino acids elongation of the ORF2-encoded Cap protein is firstly observed among PCV2 strains all over the world. The isolates were distinguished into different genotypes by PCR-RFLP methodology and antigenic changes were present in Cap protein of mutation isolates by capture ELISA.

Conclusions: The results of this study provide evidence that PCV2 is undergoing constant genetic variation and that the predominant strain in China as well as the antigenic situation has changed in recent years. Furthermore, the PCR-RFLP method presented here may be useful for the differential identification of PCV2 strains in future studies.

Show MeSH
Related in: MedlinePlus