Limits...
Protective efficacy of natansnin, a dibenzoyl glycoside from Salvinia natans against CCl4 induced oxidative stress and cellular degeneration in rat liver.

Srilaxmi P, Sareddy GR, Kavi Kishor PB, Setty OH, Babu PP - BMC Pharmacol. (2010)

Bottom Line: Natansnin treatment significantly decreased the levels of CCl4 induced apoptotic proteins and inflammatory mediators.Further natansinin treatment significantly inhibited the CCl4 induced apoptosis which was evident form the reduced TUNEL positive cells.This protective effect of natansnin can be correlated to its direct antioxidant effect.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Genetics, Osmania University, Hyderabad, India.

ABSTRACT

Background: Carbon tetra chloride (CCl4), an industrial solvent, is a hepatotoxic agent and it is the well established animal model for free radical-induced liver injury. The present investigation was carried out to establish the protective effect of natansnin, a novel dibenzoyl glycoside from Salvinia natans against CCl4 induced oxidative stress and cellular degeneration in rat liver.

Results: CCl4 significantly increased the levels of lipid peroxides, oxidized glutathione and decreased the levels of reduced glutathione, SOD and CAT. CCl4 induce marked histopathological changes and increase in the levels of apoptotic proteins. CCl4 treatment significantly increased the levels of apoptotic proteins such as caspases-3, PARP, Bax, Bid and cytochrome C and also increased the levels of inflammatory mediators iNos and Cox-2. Natansnin treatment significantly decreased the levels of CCl4 induced apoptotic proteins and inflammatory mediators. Further natansinin treatment significantly inhibited the CCl4 induced apoptosis which was evident form the reduced TUNEL positive cells.

Conclusions: In conclusion, our study demonstrated the protective effect of natansnin against CCl4 induced oxidative stress and cellular degeneration in rat liver tissue. This protective effect of natansnin can be correlated to its direct antioxidant effect.

Show MeSH

Related in: MedlinePlus

Effect of CCl4 with or without prior administration of natansnin on glutathione (oxidized & reduced) levels in liver. Reduced and oxidized glutathione levels were measured in liver homogenate from control, CCl4 and natansnin (10 mg/kg, 20 mg/kg body weight) treated rats. Values are given as percent control, and are mean ± S.D. of at least four animals. Glutathione levels are expressed as μmoles per gram tissue. The control values of GSH and GSSG were 32.12 ± 2.6, 11.3 ± 0.71 respectively. a = Statistical significant at P < 0.05 as compare to control, b = Statistical significant at P < 0.05 as compare to CCl4, c = Statistical significant at P < 0.05 as compare to CCl4+ natansnin (10 mg).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2967507&req=5

Figure 2: Effect of CCl4 with or without prior administration of natansnin on glutathione (oxidized & reduced) levels in liver. Reduced and oxidized glutathione levels were measured in liver homogenate from control, CCl4 and natansnin (10 mg/kg, 20 mg/kg body weight) treated rats. Values are given as percent control, and are mean ± S.D. of at least four animals. Glutathione levels are expressed as μmoles per gram tissue. The control values of GSH and GSSG were 32.12 ± 2.6, 11.3 ± 0.71 respectively. a = Statistical significant at P < 0.05 as compare to control, b = Statistical significant at P < 0.05 as compare to CCl4, c = Statistical significant at P < 0.05 as compare to CCl4+ natansnin (10 mg).

Mentions: Effect of CCl4 with and without the prior administration of natansnin on levels of glutathione (both oxidized and reduced) is shown in Figure 2. The levels of reduced glutathione (GSH) were decreased by 48.1% in CCl4 treated rats, when compared to controls. Natansnin treatment at 10 mg/kg body weight gave a protection rate of 41.59% and at 20 mg/kg body wt, it slightly improved it (45%). The levels of oxidized glutathione (GSSG) increased by 73% in CCl4 treated rats when compared to controls. Natansnin treatment at 10 mg/kg body wt protected the rats by 43% and at 20 mg/kg body wt up to 48%. There was a significant difference in rats administered both natansnin and CCl4 (group 4 and 5) when compared to rats administered with only CCL4. There was no significant effect on glutathione levels in rats administered with natansnin alone (group 3). The ratio of GSH/GSSG is shown in Table 3.


Protective efficacy of natansnin, a dibenzoyl glycoside from Salvinia natans against CCl4 induced oxidative stress and cellular degeneration in rat liver.

Srilaxmi P, Sareddy GR, Kavi Kishor PB, Setty OH, Babu PP - BMC Pharmacol. (2010)

Effect of CCl4 with or without prior administration of natansnin on glutathione (oxidized & reduced) levels in liver. Reduced and oxidized glutathione levels were measured in liver homogenate from control, CCl4 and natansnin (10 mg/kg, 20 mg/kg body weight) treated rats. Values are given as percent control, and are mean ± S.D. of at least four animals. Glutathione levels are expressed as μmoles per gram tissue. The control values of GSH and GSSG were 32.12 ± 2.6, 11.3 ± 0.71 respectively. a = Statistical significant at P < 0.05 as compare to control, b = Statistical significant at P < 0.05 as compare to CCl4, c = Statistical significant at P < 0.05 as compare to CCl4+ natansnin (10 mg).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2967507&req=5

Figure 2: Effect of CCl4 with or without prior administration of natansnin on glutathione (oxidized & reduced) levels in liver. Reduced and oxidized glutathione levels were measured in liver homogenate from control, CCl4 and natansnin (10 mg/kg, 20 mg/kg body weight) treated rats. Values are given as percent control, and are mean ± S.D. of at least four animals. Glutathione levels are expressed as μmoles per gram tissue. The control values of GSH and GSSG were 32.12 ± 2.6, 11.3 ± 0.71 respectively. a = Statistical significant at P < 0.05 as compare to control, b = Statistical significant at P < 0.05 as compare to CCl4, c = Statistical significant at P < 0.05 as compare to CCl4+ natansnin (10 mg).
Mentions: Effect of CCl4 with and without the prior administration of natansnin on levels of glutathione (both oxidized and reduced) is shown in Figure 2. The levels of reduced glutathione (GSH) were decreased by 48.1% in CCl4 treated rats, when compared to controls. Natansnin treatment at 10 mg/kg body weight gave a protection rate of 41.59% and at 20 mg/kg body wt, it slightly improved it (45%). The levels of oxidized glutathione (GSSG) increased by 73% in CCl4 treated rats when compared to controls. Natansnin treatment at 10 mg/kg body wt protected the rats by 43% and at 20 mg/kg body wt up to 48%. There was a significant difference in rats administered both natansnin and CCl4 (group 4 and 5) when compared to rats administered with only CCL4. There was no significant effect on glutathione levels in rats administered with natansnin alone (group 3). The ratio of GSH/GSSG is shown in Table 3.

Bottom Line: Natansnin treatment significantly decreased the levels of CCl4 induced apoptotic proteins and inflammatory mediators.Further natansinin treatment significantly inhibited the CCl4 induced apoptosis which was evident form the reduced TUNEL positive cells.This protective effect of natansnin can be correlated to its direct antioxidant effect.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Genetics, Osmania University, Hyderabad, India.

ABSTRACT

Background: Carbon tetra chloride (CCl4), an industrial solvent, is a hepatotoxic agent and it is the well established animal model for free radical-induced liver injury. The present investigation was carried out to establish the protective effect of natansnin, a novel dibenzoyl glycoside from Salvinia natans against CCl4 induced oxidative stress and cellular degeneration in rat liver.

Results: CCl4 significantly increased the levels of lipid peroxides, oxidized glutathione and decreased the levels of reduced glutathione, SOD and CAT. CCl4 induce marked histopathological changes and increase in the levels of apoptotic proteins. CCl4 treatment significantly increased the levels of apoptotic proteins such as caspases-3, PARP, Bax, Bid and cytochrome C and also increased the levels of inflammatory mediators iNos and Cox-2. Natansnin treatment significantly decreased the levels of CCl4 induced apoptotic proteins and inflammatory mediators. Further natansinin treatment significantly inhibited the CCl4 induced apoptosis which was evident form the reduced TUNEL positive cells.

Conclusions: In conclusion, our study demonstrated the protective effect of natansnin against CCl4 induced oxidative stress and cellular degeneration in rat liver tissue. This protective effect of natansnin can be correlated to its direct antioxidant effect.

Show MeSH
Related in: MedlinePlus