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Effects of olive oil and its minor phenolic constituents on obesity-induced cardiac metabolic changes.

Ebaid GM, Seiva FR, Rocha KK, Souza GA, Novelli EL - Nutr J (2010)

Bottom Line: However, a question remains to be addressed: what are the effects of olive oil and its phenolic compounds on obesity-induced cardiac metabolic changes?The present study demonstrated for the first time that olive-oil, oleuropein and cafeic-acid enhanced fat-oxidation and optimized cardiac energy metabolism in obesity conditions.Olive oil and its phenolic compounds improved myocardial oxidative stress in standard-fed conditions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Chemistry and Biochemistry, Institute of Biological Sciences, São Paulo State University, UNESP, 18618-000 Botucatu, São Paulo, Brazil.

ABSTRACT

Background: Olive oil and its minor constituents have been recommended as important dietary therapeutic interventions in preventive medicine. However, a question remains to be addressed: what are the effects of olive oil and its phenolic compounds on obesity-induced cardiac metabolic changes?

Methods: Male Wistar rats were divided into two groups (n = 24/group): (C) receiving standard-chow; (Ob) receiving hypercaloric-chow. After 21 days C and Ob groups were divided into four subgroups (n = 6/group):(C) standard-chow and saline; (C-Olive)standard-chow and olive-oil (3.0 g/kg.day); (C-Oleuropein)standard-chow and oleuropein (0.023 mg/kg/day); (C-Cafeic) standard-chow and cafeic-acid (2.66 mg/kg/day); (Ob)receiving hypercaloric-chow and saline;(Ob-Olive) hypercaloric-chow and olive-oil;(Ob-Oleuropein) hypercaloric-chow and oleuropein;(Ob-Cafeic) hypercaloric-chow and cafeic-acid. Treatments were given twice a week during 21 days.

Results: After 42 days, obesity was evidenced in Ob rats from enhanced body-weight, surface-area, and body-mass-index. Energy-expenditure, oxygen consumption(VO2) and fat-oxidation were lower in Ob-group than in C. Despite no morphometric changes, Ob-Olive, Ob-Oleuropein and Ob-Cafeic groups had higher VO2, fat-oxidation, myocardial beta-hydroxyacyl coenzyme-A dehydrogenase and lower respiratory-quotient than Ob. Citrate-synthase was highest in Ob-Olive group. Myocardial lipid-hydroperoxide(LH) and antioxidant enzymes were unaffected by olive-oil and its compounds in obesity condition, whereas LH was lower and total-antioxidant-substances were higher in C-Olive and C-Oleuropein than in C.

Conclusions: The present study demonstrated for the first time that olive-oil, oleuropein and cafeic-acid enhanced fat-oxidation and optimized cardiac energy metabolism in obesity conditions. Olive oil and its phenolic compounds improved myocardial oxidative stress in standard-fed conditions.

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Related in: MedlinePlus

Beta-hydroxyacyl coenzyme-A dehydrogenase (OHADH) activity in cardiac tissue of rats. C, control rats receiving saline; C-Olive, standard-fed rats receiving olive oil; C-Oleuropein, standard-fed rats receiving oleuropein; C-Cafeic, standard-fed rats receiving cafeic acid; Ob, Obese rats receiving saline; Ob-Olive, obese rats receiving olive oil; Ob-Oleuropein, obese rats receiving oleuropein; Ob-cafeic, obese rats receiving cafeic acid. Values are given as mean ± SD. a p < 0.05 vs. respective group. b p < 0.05 vs. respective Olive supplemented group. c p < 0.05 vs. respective Oleuropein supplemented group.
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Figure 1: Beta-hydroxyacyl coenzyme-A dehydrogenase (OHADH) activity in cardiac tissue of rats. C, control rats receiving saline; C-Olive, standard-fed rats receiving olive oil; C-Oleuropein, standard-fed rats receiving oleuropein; C-Cafeic, standard-fed rats receiving cafeic acid; Ob, Obese rats receiving saline; Ob-Olive, obese rats receiving olive oil; Ob-Oleuropein, obese rats receiving oleuropein; Ob-cafeic, obese rats receiving cafeic acid. Values are given as mean ± SD. a p < 0.05 vs. respective group. b p < 0.05 vs. respective Olive supplemented group. c p < 0.05 vs. respective Oleuropein supplemented group.

Mentions: C-cafeic rats had the lowest OHADH activity. Ob-Olive, Ob-Oleuropein and Ob-Cafeic rats had higher OHADH than Ob (Figure 1). CS was lower in C-Oleuropein than in C, as well in Ob-Olive than in Ob group (Figure 2).


Effects of olive oil and its minor phenolic constituents on obesity-induced cardiac metabolic changes.

Ebaid GM, Seiva FR, Rocha KK, Souza GA, Novelli EL - Nutr J (2010)

Beta-hydroxyacyl coenzyme-A dehydrogenase (OHADH) activity in cardiac tissue of rats. C, control rats receiving saline; C-Olive, standard-fed rats receiving olive oil; C-Oleuropein, standard-fed rats receiving oleuropein; C-Cafeic, standard-fed rats receiving cafeic acid; Ob, Obese rats receiving saline; Ob-Olive, obese rats receiving olive oil; Ob-Oleuropein, obese rats receiving oleuropein; Ob-cafeic, obese rats receiving cafeic acid. Values are given as mean ± SD. a p < 0.05 vs. respective group. b p < 0.05 vs. respective Olive supplemented group. c p < 0.05 vs. respective Oleuropein supplemented group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2967496&req=5

Figure 1: Beta-hydroxyacyl coenzyme-A dehydrogenase (OHADH) activity in cardiac tissue of rats. C, control rats receiving saline; C-Olive, standard-fed rats receiving olive oil; C-Oleuropein, standard-fed rats receiving oleuropein; C-Cafeic, standard-fed rats receiving cafeic acid; Ob, Obese rats receiving saline; Ob-Olive, obese rats receiving olive oil; Ob-Oleuropein, obese rats receiving oleuropein; Ob-cafeic, obese rats receiving cafeic acid. Values are given as mean ± SD. a p < 0.05 vs. respective group. b p < 0.05 vs. respective Olive supplemented group. c p < 0.05 vs. respective Oleuropein supplemented group.
Mentions: C-cafeic rats had the lowest OHADH activity. Ob-Olive, Ob-Oleuropein and Ob-Cafeic rats had higher OHADH than Ob (Figure 1). CS was lower in C-Oleuropein than in C, as well in Ob-Olive than in Ob group (Figure 2).

Bottom Line: However, a question remains to be addressed: what are the effects of olive oil and its phenolic compounds on obesity-induced cardiac metabolic changes?The present study demonstrated for the first time that olive-oil, oleuropein and cafeic-acid enhanced fat-oxidation and optimized cardiac energy metabolism in obesity conditions.Olive oil and its phenolic compounds improved myocardial oxidative stress in standard-fed conditions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Chemistry and Biochemistry, Institute of Biological Sciences, São Paulo State University, UNESP, 18618-000 Botucatu, São Paulo, Brazil.

ABSTRACT

Background: Olive oil and its minor constituents have been recommended as important dietary therapeutic interventions in preventive medicine. However, a question remains to be addressed: what are the effects of olive oil and its phenolic compounds on obesity-induced cardiac metabolic changes?

Methods: Male Wistar rats were divided into two groups (n = 24/group): (C) receiving standard-chow; (Ob) receiving hypercaloric-chow. After 21 days C and Ob groups were divided into four subgroups (n = 6/group):(C) standard-chow and saline; (C-Olive)standard-chow and olive-oil (3.0 g/kg.day); (C-Oleuropein)standard-chow and oleuropein (0.023 mg/kg/day); (C-Cafeic) standard-chow and cafeic-acid (2.66 mg/kg/day); (Ob)receiving hypercaloric-chow and saline;(Ob-Olive) hypercaloric-chow and olive-oil;(Ob-Oleuropein) hypercaloric-chow and oleuropein;(Ob-Cafeic) hypercaloric-chow and cafeic-acid. Treatments were given twice a week during 21 days.

Results: After 42 days, obesity was evidenced in Ob rats from enhanced body-weight, surface-area, and body-mass-index. Energy-expenditure, oxygen consumption(VO2) and fat-oxidation were lower in Ob-group than in C. Despite no morphometric changes, Ob-Olive, Ob-Oleuropein and Ob-Cafeic groups had higher VO2, fat-oxidation, myocardial beta-hydroxyacyl coenzyme-A dehydrogenase and lower respiratory-quotient than Ob. Citrate-synthase was highest in Ob-Olive group. Myocardial lipid-hydroperoxide(LH) and antioxidant enzymes were unaffected by olive-oil and its compounds in obesity condition, whereas LH was lower and total-antioxidant-substances were higher in C-Olive and C-Oleuropein than in C.

Conclusions: The present study demonstrated for the first time that olive-oil, oleuropein and cafeic-acid enhanced fat-oxidation and optimized cardiac energy metabolism in obesity conditions. Olive oil and its phenolic compounds improved myocardial oxidative stress in standard-fed conditions.

Show MeSH
Related in: MedlinePlus