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The first case of catheter-related bloodstream infection caused by Nocardia farcinica.

Heo ST, Ko KS, Kwon KT, Ryu SY, Bae IG, Oh WS, Song JH, Peck KR - J. Korean Med. Sci. (2010)

Bottom Line: Nocardia farcinica is an emerging pathogen in immunocompromised hosts.A 70-yr-old man with a tunneled central venous catheter (CVC) for home parenteral nutrition was admitted with fever for two days.Norcardia species was isolated from the blood through CVC and peripheral bloods and identified to N. farcinica by 16S rRNA and rpoB gene sequence analyses.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Jeju National University Hospital, Jeju, Korea.

ABSTRACT
Nocardia farcinica is an emerging pathogen in immunocompromised hosts. Even though several species of Nocardia have been reported as causative pathogens of catheter-related blood stream infections (CRBSI), CRBSI caused by N. farcinica has not been reported. A 70-yr-old man with a tunneled central venous catheter (CVC) for home parenteral nutrition was admitted with fever for two days. Norcardia species was isolated from the blood through CVC and peripheral bloods and identified to N. farcinica by 16S rRNA and rpoB gene sequence analyses. This report emphasizes the rapid and correct identification of causative agents in infectious diseases in the selection of antimicrobial agents and the consideration of catheter removal.

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Phylogenetic tree of SMC-A7077 and closely related species of Nocardia based on partial rpoB gene sequences. The tree was reconstructed by the neighbor-joining method, and Mycobacterium tuberculosis H37Ra was used as an outgroup. Numbers on branching nodes are percentages of 1,000 bootstrap replications. Only values of ≥50% are shown. The scale bar represents one substitution per 100 nucleotides.
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Figure 1: Phylogenetic tree of SMC-A7077 and closely related species of Nocardia based on partial rpoB gene sequences. The tree was reconstructed by the neighbor-joining method, and Mycobacterium tuberculosis H37Ra was used as an outgroup. Numbers on branching nodes are percentages of 1,000 bootstrap replications. Only values of ≥50% are shown. The scale bar represents one substitution per 100 nucleotides.

Mentions: The 16S rRNA gene sequence (1,248 bp) of the isolate showed the greatest similarity (99.92%) to N. farcinica ATCC 3318T [Gen-Bank no. Z36936], followed by N. higoensis IFM 10084T (98.80%), N. shimofusensis IFM 10311T (98.72%) and N. asiatica IFM 0245T (98.72%). The rpoB gene sequence (296 bp) of isolate SMC-A7077 showed complete identity with those of several N. farcinica strains such as DSM 43578, IFM 0284 and IFM 10152. However, it showed similarity values of 96.59% with N. shimofusensis DSM 44733T, 95.56% with N. vaccinii CIP 1775.88T, and 95.22% with N. higoensis DSM 44732T. The phylogenetic relationships of isolate SMC-A7077 with other related Nocardia strains based on rpoB gene sequences are shown in Fig. 1. Based on 16S rRNA and rpoB gene sequence analyses, we concluded that the isolate SMC-A7077 was N. farcinica.


The first case of catheter-related bloodstream infection caused by Nocardia farcinica.

Heo ST, Ko KS, Kwon KT, Ryu SY, Bae IG, Oh WS, Song JH, Peck KR - J. Korean Med. Sci. (2010)

Phylogenetic tree of SMC-A7077 and closely related species of Nocardia based on partial rpoB gene sequences. The tree was reconstructed by the neighbor-joining method, and Mycobacterium tuberculosis H37Ra was used as an outgroup. Numbers on branching nodes are percentages of 1,000 bootstrap replications. Only values of ≥50% are shown. The scale bar represents one substitution per 100 nucleotides.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2967007&req=5

Figure 1: Phylogenetic tree of SMC-A7077 and closely related species of Nocardia based on partial rpoB gene sequences. The tree was reconstructed by the neighbor-joining method, and Mycobacterium tuberculosis H37Ra was used as an outgroup. Numbers on branching nodes are percentages of 1,000 bootstrap replications. Only values of ≥50% are shown. The scale bar represents one substitution per 100 nucleotides.
Mentions: The 16S rRNA gene sequence (1,248 bp) of the isolate showed the greatest similarity (99.92%) to N. farcinica ATCC 3318T [Gen-Bank no. Z36936], followed by N. higoensis IFM 10084T (98.80%), N. shimofusensis IFM 10311T (98.72%) and N. asiatica IFM 0245T (98.72%). The rpoB gene sequence (296 bp) of isolate SMC-A7077 showed complete identity with those of several N. farcinica strains such as DSM 43578, IFM 0284 and IFM 10152. However, it showed similarity values of 96.59% with N. shimofusensis DSM 44733T, 95.56% with N. vaccinii CIP 1775.88T, and 95.22% with N. higoensis DSM 44732T. The phylogenetic relationships of isolate SMC-A7077 with other related Nocardia strains based on rpoB gene sequences are shown in Fig. 1. Based on 16S rRNA and rpoB gene sequence analyses, we concluded that the isolate SMC-A7077 was N. farcinica.

Bottom Line: Nocardia farcinica is an emerging pathogen in immunocompromised hosts.A 70-yr-old man with a tunneled central venous catheter (CVC) for home parenteral nutrition was admitted with fever for two days.Norcardia species was isolated from the blood through CVC and peripheral bloods and identified to N. farcinica by 16S rRNA and rpoB gene sequence analyses.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Jeju National University Hospital, Jeju, Korea.

ABSTRACT
Nocardia farcinica is an emerging pathogen in immunocompromised hosts. Even though several species of Nocardia have been reported as causative pathogens of catheter-related blood stream infections (CRBSI), CRBSI caused by N. farcinica has not been reported. A 70-yr-old man with a tunneled central venous catheter (CVC) for home parenteral nutrition was admitted with fever for two days. Norcardia species was isolated from the blood through CVC and peripheral bloods and identified to N. farcinica by 16S rRNA and rpoB gene sequence analyses. This report emphasizes the rapid and correct identification of causative agents in infectious diseases in the selection of antimicrobial agents and the consideration of catheter removal.

Show MeSH
Related in: MedlinePlus