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The expression of corticotropin-releasing factor in the central nucleus of the amygdala, induced by colorectal distension, is attenuated by general anesthesia.

Kim SH, Han JE, Hwang S, Oh DH - J. Korean Med. Sci. (2010)

Bottom Line: CRD elicited a significant increase in the expression of CRF and p-ERK in the CeA and of c-Fos in the spinal cord.General anesthesia attenuated the increase in CRF and p-ERK in the CeA, but did not affect the expression of spinal c-Fos.These results suggest that conscious recognition of pain at higher brain centers is an important determinant of CRF expression in the CeA.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuropsychiatry, College of Medicine and Institute of Mental Health, Hanyang University, Seoul, Korea.

ABSTRACT
Corticotrophin-releasing factor (CRF), a key regulator of the hypothalamic-pituitary axis, is expressed in the central nucleus of the amygdala (CeA) and its expression is upregulated in stress-related disorders. We investigated here the effect of noxious colorectal distension (CRD) on the expression of CRF in the CeA of conscious and unconscious rats. Adult male rats with or without general anesthesia were exposed to visceral pain induced by CRD for 5 min; this procedure was repeated 3 times with 1 min resting after each distension. The rats were sacrificed and sections of the CeA were immunostained for CRF as an indicator for anxiety response, and for phosphorylated extracellular signal-regulated kinase (p-ERK) as a marker for pain-specific activation of neurons; sections of lumbosacral spinal cord were immunostained for c-Fos as a marker for activation of spinal neurons. CRD elicited a significant increase in the expression of CRF and p-ERK in the CeA and of c-Fos in the spinal cord. General anesthesia attenuated the increase in CRF and p-ERK in the CeA, but did not affect the expression of spinal c-Fos. These results suggest that conscious recognition of pain at higher brain centers is an important determinant of CRF expression in the CeA.

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Related in: MedlinePlus

Immunostaining for phospho-ERK (pERK) in the central nucleus of the amygdala (CeA) in rats of the control group (A, CON), the colorectal distension group (B, CRD), and the CRD under general anesthesia group (C, Anesthesia+CRD). The number of pERK-positive neurons was higher in rats with CRD than in controls 30 min after CRD. The increase of pERK-positive neurons was significantly lower in rats with general anesthesia (D, number of neurons [mean±SEM] in the 5 brain sections 400 µm apart, *P<0.005 vs CON, †P<0.005 vs CRD, one-way ANOVA). Scale bar: A-C, 50 µm.
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Figure 2: Immunostaining for phospho-ERK (pERK) in the central nucleus of the amygdala (CeA) in rats of the control group (A, CON), the colorectal distension group (B, CRD), and the CRD under general anesthesia group (C, Anesthesia+CRD). The number of pERK-positive neurons was higher in rats with CRD than in controls 30 min after CRD. The increase of pERK-positive neurons was significantly lower in rats with general anesthesia (D, number of neurons [mean±SEM] in the 5 brain sections 400 µm apart, *P<0.005 vs CON, †P<0.005 vs CRD, one-way ANOVA). Scale bar: A-C, 50 µm.

Mentions: p-ERK was expressed in neurons in the CeA. After CRD, the number of pERK-positive neurons significantly increased compared to control animals. In parallel with the changes in CRF expression, the number of pERK-positive neurons was significantly reduced in anesthetized (95.17±22.47 µm2) vs conscious (264.50±65.98 µm2) rats with CRD (Fig. 2).


The expression of corticotropin-releasing factor in the central nucleus of the amygdala, induced by colorectal distension, is attenuated by general anesthesia.

Kim SH, Han JE, Hwang S, Oh DH - J. Korean Med. Sci. (2010)

Immunostaining for phospho-ERK (pERK) in the central nucleus of the amygdala (CeA) in rats of the control group (A, CON), the colorectal distension group (B, CRD), and the CRD under general anesthesia group (C, Anesthesia+CRD). The number of pERK-positive neurons was higher in rats with CRD than in controls 30 min after CRD. The increase of pERK-positive neurons was significantly lower in rats with general anesthesia (D, number of neurons [mean±SEM] in the 5 brain sections 400 µm apart, *P<0.005 vs CON, †P<0.005 vs CRD, one-way ANOVA). Scale bar: A-C, 50 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2967003&req=5

Figure 2: Immunostaining for phospho-ERK (pERK) in the central nucleus of the amygdala (CeA) in rats of the control group (A, CON), the colorectal distension group (B, CRD), and the CRD under general anesthesia group (C, Anesthesia+CRD). The number of pERK-positive neurons was higher in rats with CRD than in controls 30 min after CRD. The increase of pERK-positive neurons was significantly lower in rats with general anesthesia (D, number of neurons [mean±SEM] in the 5 brain sections 400 µm apart, *P<0.005 vs CON, †P<0.005 vs CRD, one-way ANOVA). Scale bar: A-C, 50 µm.
Mentions: p-ERK was expressed in neurons in the CeA. After CRD, the number of pERK-positive neurons significantly increased compared to control animals. In parallel with the changes in CRF expression, the number of pERK-positive neurons was significantly reduced in anesthetized (95.17±22.47 µm2) vs conscious (264.50±65.98 µm2) rats with CRD (Fig. 2).

Bottom Line: CRD elicited a significant increase in the expression of CRF and p-ERK in the CeA and of c-Fos in the spinal cord.General anesthesia attenuated the increase in CRF and p-ERK in the CeA, but did not affect the expression of spinal c-Fos.These results suggest that conscious recognition of pain at higher brain centers is an important determinant of CRF expression in the CeA.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuropsychiatry, College of Medicine and Institute of Mental Health, Hanyang University, Seoul, Korea.

ABSTRACT
Corticotrophin-releasing factor (CRF), a key regulator of the hypothalamic-pituitary axis, is expressed in the central nucleus of the amygdala (CeA) and its expression is upregulated in stress-related disorders. We investigated here the effect of noxious colorectal distension (CRD) on the expression of CRF in the CeA of conscious and unconscious rats. Adult male rats with or without general anesthesia were exposed to visceral pain induced by CRD for 5 min; this procedure was repeated 3 times with 1 min resting after each distension. The rats were sacrificed and sections of the CeA were immunostained for CRF as an indicator for anxiety response, and for phosphorylated extracellular signal-regulated kinase (p-ERK) as a marker for pain-specific activation of neurons; sections of lumbosacral spinal cord were immunostained for c-Fos as a marker for activation of spinal neurons. CRD elicited a significant increase in the expression of CRF and p-ERK in the CeA and of c-Fos in the spinal cord. General anesthesia attenuated the increase in CRF and p-ERK in the CeA, but did not affect the expression of spinal c-Fos. These results suggest that conscious recognition of pain at higher brain centers is an important determinant of CRF expression in the CeA.

Show MeSH
Related in: MedlinePlus