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Early motor balance and coordination training increased synaptophysin in subcortical regions of the ischemic rat brain.

Seo HG, Kim DY, Park HW, Lee SU, Park SH - J. Korean Med. Sci. (2010)

Bottom Line: All types of training were performed from post-operation day 1 to 14.Both Rota-rod and treadmill training increased the expression of synaptophysin in subcortical regions of the ischemic hemisphere including the hippocampus, dentate gyrus, and thalamus, but did not affect levels of brain-derived neurotrophic factor or tyrosin kinase receptor B.The control group showed significant change only in Menzies' scale score.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Seoul National University Hospital, Seoul, Korea.

ABSTRACT
The aim of this study was to evaluate the effect of early motor balance and coordination training on functional recovery and brain plasticity in an ischemic rat stroke model, compared with simple locomotor exercise. Adult male Sprague-Dawley rats with cortical infarcts were trained under one of four conditions: nontrained control, treadmill training, motor training on the Rota-rod, or both Rota-rod and treadmill training. All types of training were performed from post-operation day 1 to 14. Neurological and behavioral performance was evaluated by Menzies' scale, the prehensile test, and the limb placement test, at post-operation day 1, 7, and 14. Both Rota-rod and treadmill training increased the expression of synaptophysin in subcortical regions of the ischemic hemisphere including the hippocampus, dentate gyrus, and thalamus, but did not affect levels of brain-derived neurotrophic factor or tyrosin kinase receptor B. The Rota-rod training also improved Menzies' scale and limb placement test scores, whereas the simple treadmill training did neither. The control group showed significant change only in Menzies' scale score. This study suggests that early motor balance and coordination training may induce plastic changes in subcortical regions of the ischemic hemisphere after stroke accompanied with the recovery of sensorimotor performance.

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Synaptophysin expression by immunohistochemical staining. High-power views (×400) of the hippocampal dentate gyrus (A-D, I, J) and thalamus (E-H) show synaptophysin immunoreactivity in brown color. No definite differences are observed in the granule cell layer and subgranular zone of the contralateral hemisphere between the no exercise control (A) and Rota-rod training (B) groups. In the ischemic hemisphere, synaptophysin expression increases in the Rota-rod training group (D) compared to the control group (C). Synaptophysin immunoreactivity is higher in the thalamus of the Rota-rod training group (F, H) than in that of the control group (E, G), in particular, in the ischemic hemisphere (H). The images of the dentate hilus of the Rota-rod group (I, J) also show synaptophysin expression, particularly in the ischemic side (J).
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Figure 5: Synaptophysin expression by immunohistochemical staining. High-power views (×400) of the hippocampal dentate gyrus (A-D, I, J) and thalamus (E-H) show synaptophysin immunoreactivity in brown color. No definite differences are observed in the granule cell layer and subgranular zone of the contralateral hemisphere between the no exercise control (A) and Rota-rod training (B) groups. In the ischemic hemisphere, synaptophysin expression increases in the Rota-rod training group (D) compared to the control group (C). Synaptophysin immunoreactivity is higher in the thalamus of the Rota-rod training group (F, H) than in that of the control group (E, G), in particular, in the ischemic hemisphere (H). The images of the dentate hilus of the Rota-rod group (I, J) also show synaptophysin expression, particularly in the ischemic side (J).

Mentions: The expression of BDNF, TrkB, and synaptophysin in the four different groups (n=5 in each group) was investigated in the hippocampus, dentate gyrus, and thalamus, by immunohistochemistry. The ROD was calculated as a percentage of signals from each right brain region of the no-exercise control group. There was no significant difference in the expression of BDNF and TrkB between the groups (Fig. 4A, B). A significant increase in synaptophysin immunoreactivity was detected in the hippocampus, dentate gyrus, and thalamus of the ischemic hemisphere of the treadmill training (P<0.05) and the Rota-rod training groups (P<0.01), as compared with the no-exercise control group (Figs. 4C, 5). Compared with the contralateral brain regions, the expression of synaptophysin in the dentate gyrus and thalamus of the ischemic hemisphere increased in the Rota-rod training group (P<0.05).


Early motor balance and coordination training increased synaptophysin in subcortical regions of the ischemic rat brain.

Seo HG, Kim DY, Park HW, Lee SU, Park SH - J. Korean Med. Sci. (2010)

Synaptophysin expression by immunohistochemical staining. High-power views (×400) of the hippocampal dentate gyrus (A-D, I, J) and thalamus (E-H) show synaptophysin immunoreactivity in brown color. No definite differences are observed in the granule cell layer and subgranular zone of the contralateral hemisphere between the no exercise control (A) and Rota-rod training (B) groups. In the ischemic hemisphere, synaptophysin expression increases in the Rota-rod training group (D) compared to the control group (C). Synaptophysin immunoreactivity is higher in the thalamus of the Rota-rod training group (F, H) than in that of the control group (E, G), in particular, in the ischemic hemisphere (H). The images of the dentate hilus of the Rota-rod group (I, J) also show synaptophysin expression, particularly in the ischemic side (J).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2967002&req=5

Figure 5: Synaptophysin expression by immunohistochemical staining. High-power views (×400) of the hippocampal dentate gyrus (A-D, I, J) and thalamus (E-H) show synaptophysin immunoreactivity in brown color. No definite differences are observed in the granule cell layer and subgranular zone of the contralateral hemisphere between the no exercise control (A) and Rota-rod training (B) groups. In the ischemic hemisphere, synaptophysin expression increases in the Rota-rod training group (D) compared to the control group (C). Synaptophysin immunoreactivity is higher in the thalamus of the Rota-rod training group (F, H) than in that of the control group (E, G), in particular, in the ischemic hemisphere (H). The images of the dentate hilus of the Rota-rod group (I, J) also show synaptophysin expression, particularly in the ischemic side (J).
Mentions: The expression of BDNF, TrkB, and synaptophysin in the four different groups (n=5 in each group) was investigated in the hippocampus, dentate gyrus, and thalamus, by immunohistochemistry. The ROD was calculated as a percentage of signals from each right brain region of the no-exercise control group. There was no significant difference in the expression of BDNF and TrkB between the groups (Fig. 4A, B). A significant increase in synaptophysin immunoreactivity was detected in the hippocampus, dentate gyrus, and thalamus of the ischemic hemisphere of the treadmill training (P<0.05) and the Rota-rod training groups (P<0.01), as compared with the no-exercise control group (Figs. 4C, 5). Compared with the contralateral brain regions, the expression of synaptophysin in the dentate gyrus and thalamus of the ischemic hemisphere increased in the Rota-rod training group (P<0.05).

Bottom Line: All types of training were performed from post-operation day 1 to 14.Both Rota-rod and treadmill training increased the expression of synaptophysin in subcortical regions of the ischemic hemisphere including the hippocampus, dentate gyrus, and thalamus, but did not affect levels of brain-derived neurotrophic factor or tyrosin kinase receptor B.The control group showed significant change only in Menzies' scale score.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Seoul National University Hospital, Seoul, Korea.

ABSTRACT
The aim of this study was to evaluate the effect of early motor balance and coordination training on functional recovery and brain plasticity in an ischemic rat stroke model, compared with simple locomotor exercise. Adult male Sprague-Dawley rats with cortical infarcts were trained under one of four conditions: nontrained control, treadmill training, motor training on the Rota-rod, or both Rota-rod and treadmill training. All types of training were performed from post-operation day 1 to 14. Neurological and behavioral performance was evaluated by Menzies' scale, the prehensile test, and the limb placement test, at post-operation day 1, 7, and 14. Both Rota-rod and treadmill training increased the expression of synaptophysin in subcortical regions of the ischemic hemisphere including the hippocampus, dentate gyrus, and thalamus, but did not affect levels of brain-derived neurotrophic factor or tyrosin kinase receptor B. The Rota-rod training also improved Menzies' scale and limb placement test scores, whereas the simple treadmill training did neither. The control group showed significant change only in Menzies' scale score. This study suggests that early motor balance and coordination training may induce plastic changes in subcortical regions of the ischemic hemisphere after stroke accompanied with the recovery of sensorimotor performance.

Show MeSH
Related in: MedlinePlus