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Early motor balance and coordination training increased synaptophysin in subcortical regions of the ischemic rat brain.

Seo HG, Kim DY, Park HW, Lee SU, Park SH - J. Korean Med. Sci. (2010)

Bottom Line: All types of training were performed from post-operation day 1 to 14.Both Rota-rod and treadmill training increased the expression of synaptophysin in subcortical regions of the ischemic hemisphere including the hippocampus, dentate gyrus, and thalamus, but did not affect levels of brain-derived neurotrophic factor or tyrosin kinase receptor B.The control group showed significant change only in Menzies' scale score.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Seoul National University Hospital, Seoul, Korea.

ABSTRACT
The aim of this study was to evaluate the effect of early motor balance and coordination training on functional recovery and brain plasticity in an ischemic rat stroke model, compared with simple locomotor exercise. Adult male Sprague-Dawley rats with cortical infarcts were trained under one of four conditions: nontrained control, treadmill training, motor training on the Rota-rod, or both Rota-rod and treadmill training. All types of training were performed from post-operation day 1 to 14. Neurological and behavioral performance was evaluated by Menzies' scale, the prehensile test, and the limb placement test, at post-operation day 1, 7, and 14. Both Rota-rod and treadmill training increased the expression of synaptophysin in subcortical regions of the ischemic hemisphere including the hippocampus, dentate gyrus, and thalamus, but did not affect levels of brain-derived neurotrophic factor or tyrosin kinase receptor B. The Rota-rod training also improved Menzies' scale and limb placement test scores, whereas the simple treadmill training did neither. The control group showed significant change only in Menzies' scale score. This study suggests that early motor balance and coordination training may induce plastic changes in subcortical regions of the ischemic hemisphere after stroke accompanied with the recovery of sensorimotor performance.

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Western blot analysis. (A) Relative levels of BDNF in entire cerebral hemispheres show no significant between-group difference. A sample blot is shown; mature BDNF (about 14 kDa in size) is seen. (B) Relative levels of synaptophysin in entire cerebral hemispheres show no significant difference between groups. A sample blot is shown; synaptophysin (about 38 kDa in size) is noted.
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Figure 3: Western blot analysis. (A) Relative levels of BDNF in entire cerebral hemispheres show no significant between-group difference. A sample blot is shown; mature BDNF (about 14 kDa in size) is seen. (B) Relative levels of synaptophysin in entire cerebral hemispheres show no significant difference between groups. A sample blot is shown; synaptophysin (about 38 kDa in size) is noted.

Mentions: The four groups (n=3 for each group) were analyzed for BDNF and synaptophysin in each cerebral hemisphere using Western blotting. Bands of precursor BDNF (about 30 kDa in size) and mature BDNF (about 14 kDa) were observed. Bands of mature BDNF were quantified by densitometry. The expression of BDNF increased in the Rota-rod training group, especially in ischemic hemispheres, but this was not statistically significant (Fig. 3A). Synaptophysin bands were observed at about 38 kDa. The expression of synaptophysin showed no significant difference between groups (Fig. 3B).


Early motor balance and coordination training increased synaptophysin in subcortical regions of the ischemic rat brain.

Seo HG, Kim DY, Park HW, Lee SU, Park SH - J. Korean Med. Sci. (2010)

Western blot analysis. (A) Relative levels of BDNF in entire cerebral hemispheres show no significant between-group difference. A sample blot is shown; mature BDNF (about 14 kDa in size) is seen. (B) Relative levels of synaptophysin in entire cerebral hemispheres show no significant difference between groups. A sample blot is shown; synaptophysin (about 38 kDa in size) is noted.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2967002&req=5

Figure 3: Western blot analysis. (A) Relative levels of BDNF in entire cerebral hemispheres show no significant between-group difference. A sample blot is shown; mature BDNF (about 14 kDa in size) is seen. (B) Relative levels of synaptophysin in entire cerebral hemispheres show no significant difference between groups. A sample blot is shown; synaptophysin (about 38 kDa in size) is noted.
Mentions: The four groups (n=3 for each group) were analyzed for BDNF and synaptophysin in each cerebral hemisphere using Western blotting. Bands of precursor BDNF (about 30 kDa in size) and mature BDNF (about 14 kDa) were observed. Bands of mature BDNF were quantified by densitometry. The expression of BDNF increased in the Rota-rod training group, especially in ischemic hemispheres, but this was not statistically significant (Fig. 3A). Synaptophysin bands were observed at about 38 kDa. The expression of synaptophysin showed no significant difference between groups (Fig. 3B).

Bottom Line: All types of training were performed from post-operation day 1 to 14.Both Rota-rod and treadmill training increased the expression of synaptophysin in subcortical regions of the ischemic hemisphere including the hippocampus, dentate gyrus, and thalamus, but did not affect levels of brain-derived neurotrophic factor or tyrosin kinase receptor B.The control group showed significant change only in Menzies' scale score.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Seoul National University Hospital, Seoul, Korea.

ABSTRACT
The aim of this study was to evaluate the effect of early motor balance and coordination training on functional recovery and brain plasticity in an ischemic rat stroke model, compared with simple locomotor exercise. Adult male Sprague-Dawley rats with cortical infarcts were trained under one of four conditions: nontrained control, treadmill training, motor training on the Rota-rod, or both Rota-rod and treadmill training. All types of training were performed from post-operation day 1 to 14. Neurological and behavioral performance was evaluated by Menzies' scale, the prehensile test, and the limb placement test, at post-operation day 1, 7, and 14. Both Rota-rod and treadmill training increased the expression of synaptophysin in subcortical regions of the ischemic hemisphere including the hippocampus, dentate gyrus, and thalamus, but did not affect levels of brain-derived neurotrophic factor or tyrosin kinase receptor B. The Rota-rod training also improved Menzies' scale and limb placement test scores, whereas the simple treadmill training did neither. The control group showed significant change only in Menzies' scale score. This study suggests that early motor balance and coordination training may induce plastic changes in subcortical regions of the ischemic hemisphere after stroke accompanied with the recovery of sensorimotor performance.

Show MeSH
Related in: MedlinePlus