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Scavenging effects of dexrazoxane on free radicals.

Junjing Z, Yan Z, Baolu Z - J Clin Biochem Nutr (2010)

Bottom Line: In this study, we examined the scavenging effect of dexrazoxane on hydroxyl, superoxide, lipid, DPPH and ABTS(+) free radicals in vitro solution systems.The results demonstrated that dexrazoxane was an antioxidant that could effectively scavenge these free radicals and the scavenging effects of dexrazoxane did not require the enzymatic hydrolysis.Instead, as an effective antioxidant that has been clinically proven safe, dexrazoxane may be used in a broader spectrum of diseases that are known to be benefited by antioxidant treatments.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Academia Sinica, Bejing 100101, China.

ABSTRACT
Dexrazoxane (ICRF-187) has been clinically used to reduce doxorubicin-induced cardiotoxicity for more than 20 years. It has been proposed that dexrazoxane may act through its rings-opened hydrolysis product ADR-925, which can either remove iron from the iron-doxorubicin complex or bind to free iron, thus preventing iron-based oxygen radical formation. However, it is not known whether the antioxidant actions of dexrazoxane are totally dependent on its metabolization to its rings-opened hydrolysis product and whether dexrazoxane has any effect on the iron-independent oxygen free radical production. In this study, we examined the scavenging effect of dexrazoxane on hydroxyl, superoxide, lipid, DPPH and ABTS(+) free radicals in vitro solution systems. The results demonstrated that dexrazoxane was an antioxidant that could effectively scavenge these free radicals and the scavenging effects of dexrazoxane did not require the enzymatic hydrolysis. In addition, dexrazoxane was capable to inhibit the generation superoxide and hydroxyl radicals in iron free reaction system, indicating that the antioxidant properties of dexrazoxane were not solely dependent on iron chelation. Thus the application of dexrazoxane should not be limited to doxorubicin-induced cardiotoxicity. Instead, as an effective antioxidant that has been clinically proven safe, dexrazoxane may be used in a broader spectrum of diseases that are known to be benefited by antioxidant treatments.

No MeSH data available.


Related in: MedlinePlus

Scavenging effects of dexrazoxane on superoxide free radicals. Superoxide free radicals were generated from irradiation of riboflavin and spin trapped by DMPO. (The ESR spectrum is shown in the inset). Different concentrations of dexrazoxane were added to the reaction mixture and the generation of superoxide free radicals was determined by ESR spectroscopy. Details of the procedure are described in the “Materials and Methods” (n = 6).
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Figure 2: Scavenging effects of dexrazoxane on superoxide free radicals. Superoxide free radicals were generated from irradiation of riboflavin and spin trapped by DMPO. (The ESR spectrum is shown in the inset). Different concentrations of dexrazoxane were added to the reaction mixture and the generation of superoxide free radicals was determined by ESR spectroscopy. Details of the procedure are described in the “Materials and Methods” (n = 6).

Mentions: Superoxide free radicals generated from irradiation of riboflavin were spin trapped by DMPO and exhibited a signal with 12 lines peaks (aN = 14.2 G, aHβ = 11.2 G, aHr = 1.3 G) on ESR spectrum (inset in Fig. 2). Addition of dexrazoxane to the reaction mixture effectively decreased the ESR signal generated from superoxide free radicals (Fig. 2). The IC50 of the scavenging effect of dexrazoxane on superoxide free radical is about 0.40 mg/mL.


Scavenging effects of dexrazoxane on free radicals.

Junjing Z, Yan Z, Baolu Z - J Clin Biochem Nutr (2010)

Scavenging effects of dexrazoxane on superoxide free radicals. Superoxide free radicals were generated from irradiation of riboflavin and spin trapped by DMPO. (The ESR spectrum is shown in the inset). Different concentrations of dexrazoxane were added to the reaction mixture and the generation of superoxide free radicals was determined by ESR spectroscopy. Details of the procedure are described in the “Materials and Methods” (n = 6).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2966934&req=5

Figure 2: Scavenging effects of dexrazoxane on superoxide free radicals. Superoxide free radicals were generated from irradiation of riboflavin and spin trapped by DMPO. (The ESR spectrum is shown in the inset). Different concentrations of dexrazoxane were added to the reaction mixture and the generation of superoxide free radicals was determined by ESR spectroscopy. Details of the procedure are described in the “Materials and Methods” (n = 6).
Mentions: Superoxide free radicals generated from irradiation of riboflavin were spin trapped by DMPO and exhibited a signal with 12 lines peaks (aN = 14.2 G, aHβ = 11.2 G, aHr = 1.3 G) on ESR spectrum (inset in Fig. 2). Addition of dexrazoxane to the reaction mixture effectively decreased the ESR signal generated from superoxide free radicals (Fig. 2). The IC50 of the scavenging effect of dexrazoxane on superoxide free radical is about 0.40 mg/mL.

Bottom Line: In this study, we examined the scavenging effect of dexrazoxane on hydroxyl, superoxide, lipid, DPPH and ABTS(+) free radicals in vitro solution systems.The results demonstrated that dexrazoxane was an antioxidant that could effectively scavenge these free radicals and the scavenging effects of dexrazoxane did not require the enzymatic hydrolysis.Instead, as an effective antioxidant that has been clinically proven safe, dexrazoxane may be used in a broader spectrum of diseases that are known to be benefited by antioxidant treatments.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Academia Sinica, Bejing 100101, China.

ABSTRACT
Dexrazoxane (ICRF-187) has been clinically used to reduce doxorubicin-induced cardiotoxicity for more than 20 years. It has been proposed that dexrazoxane may act through its rings-opened hydrolysis product ADR-925, which can either remove iron from the iron-doxorubicin complex or bind to free iron, thus preventing iron-based oxygen radical formation. However, it is not known whether the antioxidant actions of dexrazoxane are totally dependent on its metabolization to its rings-opened hydrolysis product and whether dexrazoxane has any effect on the iron-independent oxygen free radical production. In this study, we examined the scavenging effect of dexrazoxane on hydroxyl, superoxide, lipid, DPPH and ABTS(+) free radicals in vitro solution systems. The results demonstrated that dexrazoxane was an antioxidant that could effectively scavenge these free radicals and the scavenging effects of dexrazoxane did not require the enzymatic hydrolysis. In addition, dexrazoxane was capable to inhibit the generation superoxide and hydroxyl radicals in iron free reaction system, indicating that the antioxidant properties of dexrazoxane were not solely dependent on iron chelation. Thus the application of dexrazoxane should not be limited to doxorubicin-induced cardiotoxicity. Instead, as an effective antioxidant that has been clinically proven safe, dexrazoxane may be used in a broader spectrum of diseases that are known to be benefited by antioxidant treatments.

No MeSH data available.


Related in: MedlinePlus