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Multiresponse strategies to modulate burst degradation and release from nanoparticles.

Sankaranarayanan J, Mahmoud EA, Kim G, Morachis JM, Almutairi A - ACS Nano (2010)

Bottom Line: The β-aminoester backbone moiety provides a pH triggered solubility switch, only when this switch is "ON" does the ketal moiety also turn "ON" to undergo rapid acid catalyzed hydrolysis.These AND logic gate polymeric nanoparticles were prepared using an oil in water emulsion method.Our results indicate that the prepared logic gate nanoparticles may prove valuable in delivering therapeutics and diagnostics to cells and diseased tissue.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Sciences, University of California at San Diego, La Jolla, California 92093-0657, USA.

ABSTRACT
Logic gate nanoparticles, where two chemical transformations take place one after the other, were successfully formulated from a newly synthesized random co-polymer. This polymer, poly([2,2'-(propane-2,2-diylbis(oxy))bis(ethane-2,1-diyl) diacrylate ]-co-[hexane-1,6-diyl diacrylate]-4,4' trimethylene dipiperidine), (poly-β-aminoester ketal-2) contains two pH responsive moieties within its backbone. As nanoparticles they function akin to an AND logic gate. The β-aminoester backbone moiety provides a pH triggered solubility switch, only when this switch is "ON" does the ketal moiety also turn "ON" to undergo rapid acid catalyzed hydrolysis. These AND logic gate polymeric nanoparticles were prepared using an oil in water emulsion method. Their degradation in the pH range of 7.4-5 was monitored by dynamic light scattering and showed excellent stability at pH 7.4 and rapid degradation at pH 5. Our results indicate that the prepared logic gate nanoparticles may prove valuable in delivering therapeutics and diagnostics to cells and diseased tissue.

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Cytotoxicity of poly-β-aminoester ketal-2 nanoparticles at different concentrations in RAW 264.7 macrophage cells.
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fig4: Cytotoxicity of poly-β-aminoester ketal-2 nanoparticles at different concentrations in RAW 264.7 macrophage cells.

Mentions: We evaluated the cytotoxicity of poly-β-aminoester ketal-2 nanoparticles in cells by a MTT assay. RAW 264.7 cells were incubated with various amounts of nanoparticles for 20 h. 4 illustrates the comparison of cytotoxicity between cells treated with increasing concentrations of poly-β-aminoester ketal-2 with respect to polymer and control cells. There was no significant cytotoxicity observed until we reached high concentrations of polymer (beyond 11.1 μg/mL).


Multiresponse strategies to modulate burst degradation and release from nanoparticles.

Sankaranarayanan J, Mahmoud EA, Kim G, Morachis JM, Almutairi A - ACS Nano (2010)

Cytotoxicity of poly-β-aminoester ketal-2 nanoparticles at different concentrations in RAW 264.7 macrophage cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2964039&req=5

fig4: Cytotoxicity of poly-β-aminoester ketal-2 nanoparticles at different concentrations in RAW 264.7 macrophage cells.
Mentions: We evaluated the cytotoxicity of poly-β-aminoester ketal-2 nanoparticles in cells by a MTT assay. RAW 264.7 cells were incubated with various amounts of nanoparticles for 20 h. 4 illustrates the comparison of cytotoxicity between cells treated with increasing concentrations of poly-β-aminoester ketal-2 with respect to polymer and control cells. There was no significant cytotoxicity observed until we reached high concentrations of polymer (beyond 11.1 μg/mL).

Bottom Line: The β-aminoester backbone moiety provides a pH triggered solubility switch, only when this switch is "ON" does the ketal moiety also turn "ON" to undergo rapid acid catalyzed hydrolysis.These AND logic gate polymeric nanoparticles were prepared using an oil in water emulsion method.Our results indicate that the prepared logic gate nanoparticles may prove valuable in delivering therapeutics and diagnostics to cells and diseased tissue.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Sciences, University of California at San Diego, La Jolla, California 92093-0657, USA.

ABSTRACT
Logic gate nanoparticles, where two chemical transformations take place one after the other, were successfully formulated from a newly synthesized random co-polymer. This polymer, poly([2,2'-(propane-2,2-diylbis(oxy))bis(ethane-2,1-diyl) diacrylate ]-co-[hexane-1,6-diyl diacrylate]-4,4' trimethylene dipiperidine), (poly-β-aminoester ketal-2) contains two pH responsive moieties within its backbone. As nanoparticles they function akin to an AND logic gate. The β-aminoester backbone moiety provides a pH triggered solubility switch, only when this switch is "ON" does the ketal moiety also turn "ON" to undergo rapid acid catalyzed hydrolysis. These AND logic gate polymeric nanoparticles were prepared using an oil in water emulsion method. Their degradation in the pH range of 7.4-5 was monitored by dynamic light scattering and showed excellent stability at pH 7.4 and rapid degradation at pH 5. Our results indicate that the prepared logic gate nanoparticles may prove valuable in delivering therapeutics and diagnostics to cells and diseased tissue.

Show MeSH
Related in: MedlinePlus