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Doxorubicin-loaded phosphatidylethanolamine-conjugated nanoliposomes: in vitro characterization and their accumulation in liver, kidneys, and lungs in rats.

Rudra A, Deepa RM, Ghosh MK, Ghosh S, Mukherjee B - Int J Nanomedicine (2010)

Bottom Line: EDX study confirmed PE-conjugation in liposomes.Data suggests that PE-conjugated nanoliposomes released the drug in a sustained manner and were capable of distributing them in various organs.This may be used for cell/ tissue targeting, attaching specific antibodies to PE.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata (Calcutta), India.

ABSTRACT

Introduction: Phosphatidylethanolamine (PE)-conjugated nanoliposomes were developed, characterized, and investigated for their accumulation in liver, kidneys, and lungs in rats.

Methods: Drug-excipient interaction was studied using Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), surface morphology by field emission scanning electron microscopy, elemental analysis by energy dispersive X-ray (EDX) analysis, zeta potential and size distribution using a Zetasizer and particle size analyzer, and in vitro drug release by dialysis membrane. In vivo accumulation of liposomes in tissues was also studied.

Results: No chemical reaction was observed between drug and excipients. EDX study confirmed PE-conjugation in liposomes. Doxorubicin-loaded liposomes (DOX-L) and PE-conjugated doxorubicin-loaded liposomes (DOX-PEL) were of smooth surface and homogenously distributed in nanosize range (32-37 nm) with a negative surface charge. Loading efficiencies were 49.25% ± 1.05% and 52.98% ± 3.22% respectively, for DOX-L and DOX-PEL. In vitro drug release study showed 69.91% ± 1.05% and 77.07% ± 1.02% doxorubicin released, from DOX-L and DOX-PEL, respectively, in nine hours. Fluorescence microscopic study showed that liposomes were well distributed in liver, lungs, and kidneys.

Conclusion: Data suggests that PE-conjugated nanoliposomes released the drug in a sustained manner and were capable of distributing them in various organs. This may be used for cell/ tissue targeting, attaching specific antibodies to PE.

Show MeSH
Fourier transform infrared spectroscopy (FTIR) spectra of A) cholesterol (CHL); B) soya-L-α-lecithin (SPC); C) doxorubicin (DOX); D) phosphatidylethanolamine (PE); E) mixture of SPC, CHL, and DOX; F) mixture of SPC, CHL, DOX, and PE; and G) lyophilized formulation (DOX-PEL).
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f1-ijn-5-811: Fourier transform infrared spectroscopy (FTIR) spectra of A) cholesterol (CHL); B) soya-L-α-lecithin (SPC); C) doxorubicin (DOX); D) phosphatidylethanolamine (PE); E) mixture of SPC, CHL, and DOX; F) mixture of SPC, CHL, DOX, and PE; and G) lyophilized formulation (DOX-PEL).

Mentions: In this study, we have initially used FTIR spectroscopy to determine any drug-excipient interaction at the level of functional groups. Spectra of CHL (Figure 1A), SPC (Figure 1B), DOX (Figure 1C), PE (Figure 1D), mixture of SPC, CHL, and DOX (Figure 1E), mixture of SPC, CHL, PE, and DOX (Figure 1F), and mixture of SPC, CHL, DOX, and PE in lyophilized formulation (Figure 1G) were compared at their different reactive functional groups in terms of peak picking.


Doxorubicin-loaded phosphatidylethanolamine-conjugated nanoliposomes: in vitro characterization and their accumulation in liver, kidneys, and lungs in rats.

Rudra A, Deepa RM, Ghosh MK, Ghosh S, Mukherjee B - Int J Nanomedicine (2010)

Fourier transform infrared spectroscopy (FTIR) spectra of A) cholesterol (CHL); B) soya-L-α-lecithin (SPC); C) doxorubicin (DOX); D) phosphatidylethanolamine (PE); E) mixture of SPC, CHL, and DOX; F) mixture of SPC, CHL, DOX, and PE; and G) lyophilized formulation (DOX-PEL).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2963929&req=5

f1-ijn-5-811: Fourier transform infrared spectroscopy (FTIR) spectra of A) cholesterol (CHL); B) soya-L-α-lecithin (SPC); C) doxorubicin (DOX); D) phosphatidylethanolamine (PE); E) mixture of SPC, CHL, and DOX; F) mixture of SPC, CHL, DOX, and PE; and G) lyophilized formulation (DOX-PEL).
Mentions: In this study, we have initially used FTIR spectroscopy to determine any drug-excipient interaction at the level of functional groups. Spectra of CHL (Figure 1A), SPC (Figure 1B), DOX (Figure 1C), PE (Figure 1D), mixture of SPC, CHL, and DOX (Figure 1E), mixture of SPC, CHL, PE, and DOX (Figure 1F), and mixture of SPC, CHL, DOX, and PE in lyophilized formulation (Figure 1G) were compared at their different reactive functional groups in terms of peak picking.

Bottom Line: EDX study confirmed PE-conjugation in liposomes.Data suggests that PE-conjugated nanoliposomes released the drug in a sustained manner and were capable of distributing them in various organs.This may be used for cell/ tissue targeting, attaching specific antibodies to PE.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata (Calcutta), India.

ABSTRACT

Introduction: Phosphatidylethanolamine (PE)-conjugated nanoliposomes were developed, characterized, and investigated for their accumulation in liver, kidneys, and lungs in rats.

Methods: Drug-excipient interaction was studied using Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), surface morphology by field emission scanning electron microscopy, elemental analysis by energy dispersive X-ray (EDX) analysis, zeta potential and size distribution using a Zetasizer and particle size analyzer, and in vitro drug release by dialysis membrane. In vivo accumulation of liposomes in tissues was also studied.

Results: No chemical reaction was observed between drug and excipients. EDX study confirmed PE-conjugation in liposomes. Doxorubicin-loaded liposomes (DOX-L) and PE-conjugated doxorubicin-loaded liposomes (DOX-PEL) were of smooth surface and homogenously distributed in nanosize range (32-37 nm) with a negative surface charge. Loading efficiencies were 49.25% ± 1.05% and 52.98% ± 3.22% respectively, for DOX-L and DOX-PEL. In vitro drug release study showed 69.91% ± 1.05% and 77.07% ± 1.02% doxorubicin released, from DOX-L and DOX-PEL, respectively, in nine hours. Fluorescence microscopic study showed that liposomes were well distributed in liver, lungs, and kidneys.

Conclusion: Data suggests that PE-conjugated nanoliposomes released the drug in a sustained manner and were capable of distributing them in various organs. This may be used for cell/ tissue targeting, attaching specific antibodies to PE.

Show MeSH