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A metabonomic comparison of urinary changes in Zucker and GK rats.

Zhao LC, Zhang XD, Liao SX, Gao HC, Wang HY, Lin DH - J. Biomed. Biotechnol. (2010)

Bottom Line: To further investigate pathogenesis and pathogenic process of type 2 diabetes mellitus (T2DM), we compared the urinary metabolic profiling of Zucker obese and Goto-kakizaki (GK) rats by NMR-based metabonomics.Simultaneously, compared with Zucker obese rats, the GK rats show decreased levels of trimethylamine, acetate, and choline, as well as increased levels of creatine/creatinine, acetoacetate, alanine, citrate, 2-ketoglutarate, succinate, lactate, and hippurate.This study demonstrates metabolic similarities between the two stages of T2DM, including reduced tricarboxylic acid (TCA) cycle and increased ketone bodies production.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Institute of Materia Medica, The Chinese Academy of Sciences, Shanghai 201203, China.

ABSTRACT
To further investigate pathogenesis and pathogenic process of type 2 diabetes mellitus (T2DM), we compared the urinary metabolic profiling of Zucker obese and Goto-kakizaki (GK) rats by NMR-based metabonomics. Principal component analysis (PCA) on urine samples of both models rats indicates markedly elevated levels of creatine/creatinine, dimethylamine, and acetoacetate, with concomitantly declined levels of citrate, 2-ketoglurarate, lactate, hippurate, and succinate compared with control rats, respectively. Simultaneously, compared with Zucker obese rats, the GK rats show decreased levels of trimethylamine, acetate, and choline, as well as increased levels of creatine/creatinine, acetoacetate, alanine, citrate, 2-ketoglutarate, succinate, lactate, and hippurate. This study demonstrates metabolic similarities between the two stages of T2DM, including reduced tricarboxylic acid (TCA) cycle and increased ketone bodies production. In addition, compared with Zucker obese rats, the GK rats have enhanced concentration of energy metabolites, which indicates energy metabolic changes produced in hyperglycemia stage more than in insulin resistance stage.

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Related in: MedlinePlus

Combined analysis of the metabolomic dataset based on the 1H NMR spectra of urine samples from Zucker obese rats (▲), Zucker lean rats (∗), GK rats (●), and Wistar rats (▵), respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig3: Combined analysis of the metabolomic dataset based on the 1H NMR spectra of urine samples from Zucker obese rats (▲), Zucker lean rats (∗), GK rats (●), and Wistar rats (▵), respectively.

Mentions: A 3D-PCA scores plot of two stages of T2DM was built using the NMR data of urine samples from both model rats and their control (Figure 3). In the plot, there is distinct separation between the different samples, which indicated differences of the metabolic patterns among different groups. Interestingly, the figure shows that Zucker obese and lean rats almost lay in the same quadrant, as well as GK and Wistar rats lay in another, which indicated the similar metabolic pattern in the similar strain of rats. From the results, we could draw the conclusion that the metabolic changes caused by strain-related variation were larger than pathological state.


A metabonomic comparison of urinary changes in Zucker and GK rats.

Zhao LC, Zhang XD, Liao SX, Gao HC, Wang HY, Lin DH - J. Biomed. Biotechnol. (2010)

Combined analysis of the metabolomic dataset based on the 1H NMR spectra of urine samples from Zucker obese rats (▲), Zucker lean rats (∗), GK rats (●), and Wistar rats (▵), respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2963802&req=5

fig3: Combined analysis of the metabolomic dataset based on the 1H NMR spectra of urine samples from Zucker obese rats (▲), Zucker lean rats (∗), GK rats (●), and Wistar rats (▵), respectively.
Mentions: A 3D-PCA scores plot of two stages of T2DM was built using the NMR data of urine samples from both model rats and their control (Figure 3). In the plot, there is distinct separation between the different samples, which indicated differences of the metabolic patterns among different groups. Interestingly, the figure shows that Zucker obese and lean rats almost lay in the same quadrant, as well as GK and Wistar rats lay in another, which indicated the similar metabolic pattern in the similar strain of rats. From the results, we could draw the conclusion that the metabolic changes caused by strain-related variation were larger than pathological state.

Bottom Line: To further investigate pathogenesis and pathogenic process of type 2 diabetes mellitus (T2DM), we compared the urinary metabolic profiling of Zucker obese and Goto-kakizaki (GK) rats by NMR-based metabonomics.Simultaneously, compared with Zucker obese rats, the GK rats show decreased levels of trimethylamine, acetate, and choline, as well as increased levels of creatine/creatinine, acetoacetate, alanine, citrate, 2-ketoglutarate, succinate, lactate, and hippurate.This study demonstrates metabolic similarities between the two stages of T2DM, including reduced tricarboxylic acid (TCA) cycle and increased ketone bodies production.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Institute of Materia Medica, The Chinese Academy of Sciences, Shanghai 201203, China.

ABSTRACT
To further investigate pathogenesis and pathogenic process of type 2 diabetes mellitus (T2DM), we compared the urinary metabolic profiling of Zucker obese and Goto-kakizaki (GK) rats by NMR-based metabonomics. Principal component analysis (PCA) on urine samples of both models rats indicates markedly elevated levels of creatine/creatinine, dimethylamine, and acetoacetate, with concomitantly declined levels of citrate, 2-ketoglurarate, lactate, hippurate, and succinate compared with control rats, respectively. Simultaneously, compared with Zucker obese rats, the GK rats show decreased levels of trimethylamine, acetate, and choline, as well as increased levels of creatine/creatinine, acetoacetate, alanine, citrate, 2-ketoglutarate, succinate, lactate, and hippurate. This study demonstrates metabolic similarities between the two stages of T2DM, including reduced tricarboxylic acid (TCA) cycle and increased ketone bodies production. In addition, compared with Zucker obese rats, the GK rats have enhanced concentration of energy metabolites, which indicates energy metabolic changes produced in hyperglycemia stage more than in insulin resistance stage.

Show MeSH
Related in: MedlinePlus