Mesenchymal-to-epithelial transition of intercalating cells in Drosophila renal tubules depends on polarity cues from epithelial neighbours.
Bottom Line: We also show that expression of basolateral proteins in stellate cells is a prerequisite for their ingression between principal cells.We present a model in which the contacts with successive principal cell membrane domains made by stellate cells as they integrate between them act as a cue for the elaboration of stellate cell polarity.We suggest that the formation of zonula adherens junctions between new cell neighbours establishes their apico-basal positions and stabilises them in the epithelium.
Affiliation: Institut de Biologia Molecular de Barcelona-CSIC, Parc Cientific de Barcelona, 08028 Barcelona, Spain.Show MeSH
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Mentions: In wild-type tubules of early stage 14 embryos, stellate cells are located around the tubule lumen, and their apical domains and adherens junctions are in register with neighbouring principal cells (Figs. 3H–J and 4A–D). Remarkably, in embryos in which polarity and adherens junctions have been disrupted in the renal tubules, the stellate cells are located in the middle of the tubules, in the position of the lumen (Fig. 4E–H, arrows). Thus apico-basal polarity and adherens junctions in principal cells are required in the tubule epithelium for the stellate cells to stop moving through the tubule when they reach the apical surface, and to keep contact at their basal side with the outside of the tubule.
Affiliation: Institut de Biologia Molecular de Barcelona-CSIC, Parc Cientific de Barcelona, 08028 Barcelona, Spain.