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Mesenchymal-to-epithelial transition of intercalating cells in Drosophila renal tubules depends on polarity cues from epithelial neighbours.

Campbell K, Casanova J, Skaer H - Mech. Dev. (2010)

Bottom Line: We also show that expression of basolateral proteins in stellate cells is a prerequisite for their ingression between principal cells.We present a model in which the contacts with successive principal cell membrane domains made by stellate cells as they integrate between them act as a cue for the elaboration of stellate cell polarity.We suggest that the formation of zonula adherens junctions between new cell neighbours establishes their apico-basal positions and stabilises them in the epithelium.

View Article: PubMed Central - PubMed

Affiliation: Institut de Biologia Molecular de Barcelona-CSIC, Parc Cientific de Barcelona, 08028 Barcelona, Spain.

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Overexpression of Crb in the renal epithelium disrupts the integration and polarisation of stellate cells. (A and H) Sequential 1.5 μm thick confocal z-sections of renal tubules were used to analyse the positioning of stellate cells in stage 14 wild-type (A–D) and Crb-overexpressing (E–H) tubules stained for Tsh (red) and Crb (green). (A–D and I) In wild-type embryos stellate cells are found around the tubule lumen. (E–H and J) In Crb-overexpressing tubules stellate cells are located in the middle of the tubules in the position of the lumen, arrows in (E–H) point to the lumen. (K–L) Stage 15 Crb-overexpressing tubules stained for Crb (blue), Tsh (red) and E-Cad (K, green) or Dlg (L, green). Stellate cells exhibit varying degrees of polarity, ranging from a complete lack of expression of Crb and E-Cad (K, arrow) to low levels of Crb, E-Cad and Dlg, which are not correctly orientated with respect to the apico-basal axis of the tissue (K, L, arrowheads). White lines outline the tubules.
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fig4: Overexpression of Crb in the renal epithelium disrupts the integration and polarisation of stellate cells. (A and H) Sequential 1.5 μm thick confocal z-sections of renal tubules were used to analyse the positioning of stellate cells in stage 14 wild-type (A–D) and Crb-overexpressing (E–H) tubules stained for Tsh (red) and Crb (green). (A–D and I) In wild-type embryos stellate cells are found around the tubule lumen. (E–H and J) In Crb-overexpressing tubules stellate cells are located in the middle of the tubules in the position of the lumen, arrows in (E–H) point to the lumen. (K–L) Stage 15 Crb-overexpressing tubules stained for Crb (blue), Tsh (red) and E-Cad (K, green) or Dlg (L, green). Stellate cells exhibit varying degrees of polarity, ranging from a complete lack of expression of Crb and E-Cad (K, arrow) to low levels of Crb, E-Cad and Dlg, which are not correctly orientated with respect to the apico-basal axis of the tissue (K, L, arrowheads). White lines outline the tubules.

Mentions: In wild-type tubules of early stage 14 embryos, stellate cells are located around the tubule lumen, and their apical domains and adherens junctions are in register with neighbouring principal cells (Figs. 3H–J and 4A–D). Remarkably, in embryos in which polarity and adherens junctions have been disrupted in the renal tubules, the stellate cells are located in the middle of the tubules, in the position of the lumen (Fig. 4E–H, arrows). Thus apico-basal polarity and adherens junctions in principal cells are required in the tubule epithelium for the stellate cells to stop moving through the tubule when they reach the apical surface, and to keep contact at their basal side with the outside of the tubule.


Mesenchymal-to-epithelial transition of intercalating cells in Drosophila renal tubules depends on polarity cues from epithelial neighbours.

Campbell K, Casanova J, Skaer H - Mech. Dev. (2010)

Overexpression of Crb in the renal epithelium disrupts the integration and polarisation of stellate cells. (A and H) Sequential 1.5 μm thick confocal z-sections of renal tubules were used to analyse the positioning of stellate cells in stage 14 wild-type (A–D) and Crb-overexpressing (E–H) tubules stained for Tsh (red) and Crb (green). (A–D and I) In wild-type embryos stellate cells are found around the tubule lumen. (E–H and J) In Crb-overexpressing tubules stellate cells are located in the middle of the tubules in the position of the lumen, arrows in (E–H) point to the lumen. (K–L) Stage 15 Crb-overexpressing tubules stained for Crb (blue), Tsh (red) and E-Cad (K, green) or Dlg (L, green). Stellate cells exhibit varying degrees of polarity, ranging from a complete lack of expression of Crb and E-Cad (K, arrow) to low levels of Crb, E-Cad and Dlg, which are not correctly orientated with respect to the apico-basal axis of the tissue (K, L, arrowheads). White lines outline the tubules.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2963794&req=5

fig4: Overexpression of Crb in the renal epithelium disrupts the integration and polarisation of stellate cells. (A and H) Sequential 1.5 μm thick confocal z-sections of renal tubules were used to analyse the positioning of stellate cells in stage 14 wild-type (A–D) and Crb-overexpressing (E–H) tubules stained for Tsh (red) and Crb (green). (A–D and I) In wild-type embryos stellate cells are found around the tubule lumen. (E–H and J) In Crb-overexpressing tubules stellate cells are located in the middle of the tubules in the position of the lumen, arrows in (E–H) point to the lumen. (K–L) Stage 15 Crb-overexpressing tubules stained for Crb (blue), Tsh (red) and E-Cad (K, green) or Dlg (L, green). Stellate cells exhibit varying degrees of polarity, ranging from a complete lack of expression of Crb and E-Cad (K, arrow) to low levels of Crb, E-Cad and Dlg, which are not correctly orientated with respect to the apico-basal axis of the tissue (K, L, arrowheads). White lines outline the tubules.
Mentions: In wild-type tubules of early stage 14 embryos, stellate cells are located around the tubule lumen, and their apical domains and adherens junctions are in register with neighbouring principal cells (Figs. 3H–J and 4A–D). Remarkably, in embryos in which polarity and adherens junctions have been disrupted in the renal tubules, the stellate cells are located in the middle of the tubules, in the position of the lumen (Fig. 4E–H, arrows). Thus apico-basal polarity and adherens junctions in principal cells are required in the tubule epithelium for the stellate cells to stop moving through the tubule when they reach the apical surface, and to keep contact at their basal side with the outside of the tubule.

Bottom Line: We also show that expression of basolateral proteins in stellate cells is a prerequisite for their ingression between principal cells.We present a model in which the contacts with successive principal cell membrane domains made by stellate cells as they integrate between them act as a cue for the elaboration of stellate cell polarity.We suggest that the formation of zonula adherens junctions between new cell neighbours establishes their apico-basal positions and stabilises them in the epithelium.

View Article: PubMed Central - PubMed

Affiliation: Institut de Biologia Molecular de Barcelona-CSIC, Parc Cientific de Barcelona, 08028 Barcelona, Spain.

Show MeSH
Related in: MedlinePlus