Heterocellular induction of interferon by negative-sense RNA viruses.
Bottom Line: The infection of cells by RNA viruses is associated with the recognition of virus PAMPs (pathogen-associated molecular patterns) and the production of type I interferon (IFN).Here we present data on the dynamics of IFN production and response during developing infections by paramyxoviruses, influenza A virus and bunyamwera virus.We show that only a limited number of infected cells are responsible for the production of IFN, and that this heterocellular production is a feature of the infecting virus as opposed to an intrinsic property of the cells.
Affiliation: School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St. Andrews, St. Andrews, Fife, KY16 9ST, UK.Show MeSH
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Mentions: The data above show that efficient inducers of IFN-β (i.e. a MuV preparation rich in DI particles, or synthetic PAMPs; data not shown) cause a robust induction of GFP expression in the majority of A549/pr(IFN-β).GFP cells, with responsive cells producing similar levels of fluorescence. We next used the A549/pr(IFN-β).GFP cells to monitor the activation of the IFN-β promoter in individual cells during infection by viruses generated by passage at low m.o.i. Such preparations would be typical working stocks, lacking or low in DI particles, and are generally poor inducers of IFN-β (see for example Poole et al., 2002). Strikingly, in contrast to the extensive induction seen by the DI-rich preparation, MuV(ori) — see Fig. 2, a plaque-purified preparation of MuV, termed MuV cl3/30 (Young et al., 2009), showed high infectivity but only generated a few GFP-positive cells (Fig. 3). Each of the GFP-positive cells showed a similar degree of fluorescence, as described above for the MuV(ori) infection.
Affiliation: School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St. Andrews, St. Andrews, Fife, KY16 9ST, UK.