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Presence of celiac disease epitopes in modern and old hexaploid wheat varieties: wheat breeding may have contributed to increased prevalence of celiac disease.

van den Broeck HC, de Jong HC, Salentijn EM, Dekking L, Bosch D, Hamer RJ, Gilissen LJ, van der Meer IM, Smulders MJ - Theor. Appl. Genet. (2010)

Bottom Line: The minor Glia-α20 was included as a technical reference.Overall, the presence of the Glia-α9 epitope was higher in the modern varieties, whereas the presence of the Glia-α20 epitope was lower, as compared to the landraces.Large-scale culture and consumption of such varieties would considerably aid in decreasing the prevalence of CD.

View Article: PubMed Central - PubMed

Affiliation: Plant Research International, Wageningen UR, P.O. Box 16, 6700 AA, Wageningen, The Netherlands. hetty.busink@wur.nl

ABSTRACT
Gluten proteins from wheat can induce celiac disease (CD) in genetically susceptible individuals. Specific gluten peptides can be presented by antigen presenting cells to gluten-sensitive T-cell lymphocytes leading to CD. During the last decades, a significant increase has been observed in the prevalence of CD. This may partly be attributed to an increase in awareness and to improved diagnostic techniques, but increased wheat and gluten consumption is also considered a major cause. To analyze whether wheat breeding contributed to the increase of the prevalence of CD, we have compared the genetic diversity of gluten proteins for the presence of two CD epitopes (Glia-α9 and Glia-α20) in 36 modern European wheat varieties and in 50 landraces representing the wheat varieties grown up to around a century ago. Glia-α9 is a major (immunodominant) epitope that is recognized by the majority of CD patients. The minor Glia-α20 was included as a technical reference. Overall, the presence of the Glia-α9 epitope was higher in the modern varieties, whereas the presence of the Glia-α20 epitope was lower, as compared to the landraces. This suggests that modern wheat breeding practices may have led to an increased exposure to CD epitopes. On the other hand, some modern varieties and landraces have been identified that have relatively low contents of both epitopes. Such selected lines may serve as a start to breed wheat for the introduction of 'low CD toxic' as a new breeding trait. Large-scale culture and consumption of such varieties would considerably aid in decreasing the prevalence of CD.

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Related in: MedlinePlus

Immunoblots from Fig. 1 were scanned and the relative intensities are shown for mAbs Glia-α9 and Glia-α20. a Glia-α9 in modern wheat varieties, b Glia-α20 in modern wheat varieties, c Glia-α9 in wheat landraces, and d Glia-α20 in wheat landraces
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Fig4: Immunoblots from Fig. 1 were scanned and the relative intensities are shown for mAbs Glia-α9 and Glia-α20. a Glia-α9 in modern wheat varieties, b Glia-α20 in modern wheat varieties, c Glia-α9 in wheat landraces, and d Glia-α20 in wheat landraces

Mentions: The results for the landraces showed much more diverse protein patterns for proteins that bind Glia-α9 or Glia-α20 mAb (Fig. 4a, b). The two closely linked ω-gliadins/D-type LMW-GS were also present in the landraces (boxed proteins in Fig. 1c, d). In some landraces, both the higher and lower molecular mass ω-gliadins/D-type LMW-GS were present. This may be caused by genetic heterogeneity within the accessions that were obtained from a Genebank, since several kernels were combined for protein extraction. The ‘block-2’ gluten proteins were not found in any of the landraces we have tested.Fig. 4


Presence of celiac disease epitopes in modern and old hexaploid wheat varieties: wheat breeding may have contributed to increased prevalence of celiac disease.

van den Broeck HC, de Jong HC, Salentijn EM, Dekking L, Bosch D, Hamer RJ, Gilissen LJ, van der Meer IM, Smulders MJ - Theor. Appl. Genet. (2010)

Immunoblots from Fig. 1 were scanned and the relative intensities are shown for mAbs Glia-α9 and Glia-α20. a Glia-α9 in modern wheat varieties, b Glia-α20 in modern wheat varieties, c Glia-α9 in wheat landraces, and d Glia-α20 in wheat landraces
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2963738&req=5

Fig4: Immunoblots from Fig. 1 were scanned and the relative intensities are shown for mAbs Glia-α9 and Glia-α20. a Glia-α9 in modern wheat varieties, b Glia-α20 in modern wheat varieties, c Glia-α9 in wheat landraces, and d Glia-α20 in wheat landraces
Mentions: The results for the landraces showed much more diverse protein patterns for proteins that bind Glia-α9 or Glia-α20 mAb (Fig. 4a, b). The two closely linked ω-gliadins/D-type LMW-GS were also present in the landraces (boxed proteins in Fig. 1c, d). In some landraces, both the higher and lower molecular mass ω-gliadins/D-type LMW-GS were present. This may be caused by genetic heterogeneity within the accessions that were obtained from a Genebank, since several kernels were combined for protein extraction. The ‘block-2’ gluten proteins were not found in any of the landraces we have tested.Fig. 4

Bottom Line: The minor Glia-α20 was included as a technical reference.Overall, the presence of the Glia-α9 epitope was higher in the modern varieties, whereas the presence of the Glia-α20 epitope was lower, as compared to the landraces.Large-scale culture and consumption of such varieties would considerably aid in decreasing the prevalence of CD.

View Article: PubMed Central - PubMed

Affiliation: Plant Research International, Wageningen UR, P.O. Box 16, 6700 AA, Wageningen, The Netherlands. hetty.busink@wur.nl

ABSTRACT
Gluten proteins from wheat can induce celiac disease (CD) in genetically susceptible individuals. Specific gluten peptides can be presented by antigen presenting cells to gluten-sensitive T-cell lymphocytes leading to CD. During the last decades, a significant increase has been observed in the prevalence of CD. This may partly be attributed to an increase in awareness and to improved diagnostic techniques, but increased wheat and gluten consumption is also considered a major cause. To analyze whether wheat breeding contributed to the increase of the prevalence of CD, we have compared the genetic diversity of gluten proteins for the presence of two CD epitopes (Glia-α9 and Glia-α20) in 36 modern European wheat varieties and in 50 landraces representing the wheat varieties grown up to around a century ago. Glia-α9 is a major (immunodominant) epitope that is recognized by the majority of CD patients. The minor Glia-α20 was included as a technical reference. Overall, the presence of the Glia-α9 epitope was higher in the modern varieties, whereas the presence of the Glia-α20 epitope was lower, as compared to the landraces. This suggests that modern wheat breeding practices may have led to an increased exposure to CD epitopes. On the other hand, some modern varieties and landraces have been identified that have relatively low contents of both epitopes. Such selected lines may serve as a start to breed wheat for the introduction of 'low CD toxic' as a new breeding trait. Large-scale culture and consumption of such varieties would considerably aid in decreasing the prevalence of CD.

Show MeSH
Related in: MedlinePlus