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The role of LCCL proteins in malaria transmission

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The type member of this family in P. berghei, named PbSR or PbLAP1, is synthesized in macrogametocytes, gets trafficked to the ookinete's crystalloid organelles, and is essential for crystalloid organelle formation... To study the expression and localization, genetically modified parasite lines were generated expressing PbLAP3 tagged with green fluorescent protein (GFP) at the C-terminus... Life stage expression in ookinete was studied by setting up ookinete culture, expression in oocyst and sporozoite was assessed by mosquito infection... Analysis of GFP fluorescence of PbLAP3/EGFP by confocal microscopy revealed PbLAP3 expression in gametocytes (Figure 1a)... In ookinetes GFP fluorescence was observed as two cytoplasmic focal spots (Figure 1b)... These spots are characteristic for the crystalloids... Green fluorescence was not detected in oocysts, nor in sporozoites of parasite line PbLAP3/EGFP, which indicate that PbLAP3 is not expressed in these life stages... LCCL proteins play a central role in the function of crystalloids in P. berghei... The new cellular markers for the crystalloids identified here will provide useful new tools for addressing intriguing questions about the molecular processes that lead to crystalloids formation which then facilitate the sporozoite development.

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Expression and subcellular localisation of GFP-tagged PbLAP3 in gametocytes and ookinetes of Plasmodium berghei.
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Figure 1: Expression and subcellular localisation of GFP-tagged PbLAP3 in gametocytes and ookinetes of Plasmodium berghei.

Mentions: Analysis of GFP fluorescence of PbLAP3/EGFP by confocal microscopy revealed PbLAP3 expression in gametocytes (Figure 1a) [2]. In ookinetes GFP fluorescence was observed as two cytoplasmic focal spots (Figure 1b) [2]. These spots are characteristic for the crystalloids [1]. Green fluorescence was not detected in oocysts, nor in sporozoites of parasite line PbLAP3/EGFP, which indicate that PbLAP3 is not expressed in these life stages [2].


The role of LCCL proteins in malaria transmission
Expression and subcellular localisation of GFP-tagged PbLAP3 in gametocytes and ookinetes of Plasmodium berghei.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2963235&req=5

Figure 1: Expression and subcellular localisation of GFP-tagged PbLAP3 in gametocytes and ookinetes of Plasmodium berghei.
Mentions: Analysis of GFP fluorescence of PbLAP3/EGFP by confocal microscopy revealed PbLAP3 expression in gametocytes (Figure 1a) [2]. In ookinetes GFP fluorescence was observed as two cytoplasmic focal spots (Figure 1b) [2]. These spots are characteristic for the crystalloids [1]. Green fluorescence was not detected in oocysts, nor in sporozoites of parasite line PbLAP3/EGFP, which indicate that PbLAP3 is not expressed in these life stages [2].

View Article: PubMed Central - HTML

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

The type member of this family in P. berghei, named PbSR or PbLAP1, is synthesized in macrogametocytes, gets trafficked to the ookinete's crystalloid organelles, and is essential for crystalloid organelle formation... To study the expression and localization, genetically modified parasite lines were generated expressing PbLAP3 tagged with green fluorescent protein (GFP) at the C-terminus... Life stage expression in ookinete was studied by setting up ookinete culture, expression in oocyst and sporozoite was assessed by mosquito infection... Analysis of GFP fluorescence of PbLAP3/EGFP by confocal microscopy revealed PbLAP3 expression in gametocytes (Figure 1a)... In ookinetes GFP fluorescence was observed as two cytoplasmic focal spots (Figure 1b)... These spots are characteristic for the crystalloids... Green fluorescence was not detected in oocysts, nor in sporozoites of parasite line PbLAP3/EGFP, which indicate that PbLAP3 is not expressed in these life stages... LCCL proteins play a central role in the function of crystalloids in P. berghei... The new cellular markers for the crystalloids identified here will provide useful new tools for addressing intriguing questions about the molecular processes that lead to crystalloids formation which then facilitate the sporozoite development.

No MeSH data available.