Limits...
CpG oligodeoxynucleotides enhance the efficacy of adoptive cell transfer using tumor infiltrating lymphocytes by modifying the Th1 polarization and local infiltration of Th17 cells.

Xu L, Wang C, Wen Z, Zhou Y, Liu Z, Liang Y, Xu Z, Ren T - Clin. Dev. Immunol. (2010)

Bottom Line: But the efficacy remains limited and development of new strategies is urgent.Recent evidence suggested that CpG-ODNs might be a potent candidate for tumor immunotherapy.Most importantly, we found that CpG-ODNs could significantly elevate the infiltration of Th17 cells in tumor mass, which contributed to anti-tumor efficacy of TILs in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Zunyi Medical College, Guizhou, China.

ABSTRACT
Adoptive cell transfer immunotherapy using tumor infiltrating lymphocytes (TILs) was an important therapeutic strategy against tumors. But the efficacy remains limited and development of new strategies is urgent. Recent evidence suggested that CpG-ODNs might be a potent candidate for tumor immunotherapy. Here we firstly reported that CpG-ODNs could significantly enhance the antitumor efficacy of adoptively transferred TILs in vivo accompanied by enhanced activity capacity and proliferation of CD8(+) T cells and CD8(+) T cells, as well as a Th1 polarization immune response. Most importantly, we found that CpG-ODNs could significantly elevate the infiltration of Th17 cells in tumor mass, which contributed to anti-tumor efficacy of TILs in vivo. Our findings suggested that CpG ODNs could enhance the anti-tumor efficacy of adoptively transferred TILs through modifying Th1 polarization and local infiltration of Th17 cells, which might provide a clue for developing a new strategy for ACT based on TILs.

Show MeSH

Related in: MedlinePlus

Neutralization of IL-17 reduced the survival of human lung cancer bearing nude mice. TILs were collected from lung cancer patients (n = 12) and treated with CpG-ODNs or control CpG-ODNs as described in Materials and Methods. 1 × 107 autologous TILs were transferred into the tumor bearing Balb/c nude mice with 100 ug CpG-ODNs or control CpG-ODNs through tail vein. After 2 days, the recipients were injected intravenously with anti-IL-17 (5 ug/g) every seven days. Then, the survival of tumor bearing nude mice was observed.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2963116&req=5

fig6: Neutralization of IL-17 reduced the survival of human lung cancer bearing nude mice. TILs were collected from lung cancer patients (n = 12) and treated with CpG-ODNs or control CpG-ODNs as described in Materials and Methods. 1 × 107 autologous TILs were transferred into the tumor bearing Balb/c nude mice with 100 ug CpG-ODNs or control CpG-ODNs through tail vein. After 2 days, the recipients were injected intravenously with anti-IL-17 (5 ug/g) every seven days. Then, the survival of tumor bearing nude mice was observed.

Mentions: To evaluate the potential role of Th17 cells in the enhanced antitumor responses of TILs induced by CpG-ODNs, two days after transfer, the recipients were injected intravenously with anti-IL-17 (5 ug/g) every seven days, and then the survival time of tumor-bearing mice were observed. Compared with that of control groups, the survival time in mice treated with IL-17 neutralizing antibodies was significantly reduced (Figure 6, day 40 versus days 48 and 49, P < .05), indicating that Th17 cells might play an important role in the enhanced antitumor efficacy of TILs induced by CpG-ODNs.


CpG oligodeoxynucleotides enhance the efficacy of adoptive cell transfer using tumor infiltrating lymphocytes by modifying the Th1 polarization and local infiltration of Th17 cells.

Xu L, Wang C, Wen Z, Zhou Y, Liu Z, Liang Y, Xu Z, Ren T - Clin. Dev. Immunol. (2010)

Neutralization of IL-17 reduced the survival of human lung cancer bearing nude mice. TILs were collected from lung cancer patients (n = 12) and treated with CpG-ODNs or control CpG-ODNs as described in Materials and Methods. 1 × 107 autologous TILs were transferred into the tumor bearing Balb/c nude mice with 100 ug CpG-ODNs or control CpG-ODNs through tail vein. After 2 days, the recipients were injected intravenously with anti-IL-17 (5 ug/g) every seven days. Then, the survival of tumor bearing nude mice was observed.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2963116&req=5

fig6: Neutralization of IL-17 reduced the survival of human lung cancer bearing nude mice. TILs were collected from lung cancer patients (n = 12) and treated with CpG-ODNs or control CpG-ODNs as described in Materials and Methods. 1 × 107 autologous TILs were transferred into the tumor bearing Balb/c nude mice with 100 ug CpG-ODNs or control CpG-ODNs through tail vein. After 2 days, the recipients were injected intravenously with anti-IL-17 (5 ug/g) every seven days. Then, the survival of tumor bearing nude mice was observed.
Mentions: To evaluate the potential role of Th17 cells in the enhanced antitumor responses of TILs induced by CpG-ODNs, two days after transfer, the recipients were injected intravenously with anti-IL-17 (5 ug/g) every seven days, and then the survival time of tumor-bearing mice were observed. Compared with that of control groups, the survival time in mice treated with IL-17 neutralizing antibodies was significantly reduced (Figure 6, day 40 versus days 48 and 49, P < .05), indicating that Th17 cells might play an important role in the enhanced antitumor efficacy of TILs induced by CpG-ODNs.

Bottom Line: But the efficacy remains limited and development of new strategies is urgent.Recent evidence suggested that CpG-ODNs might be a potent candidate for tumor immunotherapy.Most importantly, we found that CpG-ODNs could significantly elevate the infiltration of Th17 cells in tumor mass, which contributed to anti-tumor efficacy of TILs in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Zunyi Medical College, Guizhou, China.

ABSTRACT
Adoptive cell transfer immunotherapy using tumor infiltrating lymphocytes (TILs) was an important therapeutic strategy against tumors. But the efficacy remains limited and development of new strategies is urgent. Recent evidence suggested that CpG-ODNs might be a potent candidate for tumor immunotherapy. Here we firstly reported that CpG-ODNs could significantly enhance the antitumor efficacy of adoptively transferred TILs in vivo accompanied by enhanced activity capacity and proliferation of CD8(+) T cells and CD8(+) T cells, as well as a Th1 polarization immune response. Most importantly, we found that CpG-ODNs could significantly elevate the infiltration of Th17 cells in tumor mass, which contributed to anti-tumor efficacy of TILs in vivo. Our findings suggested that CpG ODNs could enhance the anti-tumor efficacy of adoptively transferred TILs through modifying Th1 polarization and local infiltration of Th17 cells, which might provide a clue for developing a new strategy for ACT based on TILs.

Show MeSH
Related in: MedlinePlus