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High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct.

de Castro AM, Ferreira MC, da Cruz JC, Pedro KC, Carvalho DF, Leite SG, Pereira N - Enzyme Res (2010)

Bottom Line: The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol.In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation.The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50°C, accessory xylanase activity, and absence of proteolytic activity towards azocasein.

View Article: PubMed Central - PubMed

Affiliation: Renewable Energy Division, Research and Development Center, Petrobras, Avenue Horácio Macedo, 950 Ilha do Fundão, 21941-915 Rio de Janeiro, RJ, Brazil.

ABSTRACT
The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol. In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation. This fungal strain produced high contents of endoglucanase activity (6,358 U·L(-1)) after 72 hours of process, and further relevant β-glucosidase and FPase activities (742 and 445 U·L(-1), resp.). The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50°C, accessory xylanase activity, and absence of proteolytic activity towards azocasein. This strain showed, therefore, potential for the production of complete cellulolytic complexes aiming at the saccharification of lignocellulosic materials.

No MeSH data available.


Related in: MedlinePlus

Thermal stability of FPase (triangles), endoglucanase (squares), and β-glucosidase (circles) produced by T. harzianum at 37°C (black and white symbols), 50°C (white symbols), and 60°C (black symbols).
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fig3: Thermal stability of FPase (triangles), endoglucanase (squares), and β-glucosidase (circles) produced by T. harzianum at 37°C (black and white symbols), 50°C (white symbols), and 60°C (black symbols).

Mentions: The investigation of stability of the enzymes produced by SmF of T. harzianum at three temperatures was carried out during 23 h, which is a period enough or even longer to reach a stationary phase for ethanol production in SSF processes [16, 17]. Profiles of relative enzymatic activity are presented in Figure 3. As expected, the higher the temperature, the higher the loss in activity. β-glucosidase activity was the most sensitive to the two highest incubation temperatures, 50 and 60°C, presenting half life times of about 4 h and less than 1 h, respectively. At 37°C all enzymes produced by T. harzianum maintained at least 90% of their initial activities after 23 h of incubation.


High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct.

de Castro AM, Ferreira MC, da Cruz JC, Pedro KC, Carvalho DF, Leite SG, Pereira N - Enzyme Res (2010)

Thermal stability of FPase (triangles), endoglucanase (squares), and β-glucosidase (circles) produced by T. harzianum at 37°C (black and white symbols), 50°C (white symbols), and 60°C (black symbols).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2962913&req=5

fig3: Thermal stability of FPase (triangles), endoglucanase (squares), and β-glucosidase (circles) produced by T. harzianum at 37°C (black and white symbols), 50°C (white symbols), and 60°C (black symbols).
Mentions: The investigation of stability of the enzymes produced by SmF of T. harzianum at three temperatures was carried out during 23 h, which is a period enough or even longer to reach a stationary phase for ethanol production in SSF processes [16, 17]. Profiles of relative enzymatic activity are presented in Figure 3. As expected, the higher the temperature, the higher the loss in activity. β-glucosidase activity was the most sensitive to the two highest incubation temperatures, 50 and 60°C, presenting half life times of about 4 h and less than 1 h, respectively. At 37°C all enzymes produced by T. harzianum maintained at least 90% of their initial activities after 23 h of incubation.

Bottom Line: The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol.In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation.The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50°C, accessory xylanase activity, and absence of proteolytic activity towards azocasein.

View Article: PubMed Central - PubMed

Affiliation: Renewable Energy Division, Research and Development Center, Petrobras, Avenue Horácio Macedo, 950 Ilha do Fundão, 21941-915 Rio de Janeiro, RJ, Brazil.

ABSTRACT
The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol. In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation. This fungal strain produced high contents of endoglucanase activity (6,358 U·L(-1)) after 72 hours of process, and further relevant β-glucosidase and FPase activities (742 and 445 U·L(-1), resp.). The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50°C, accessory xylanase activity, and absence of proteolytic activity towards azocasein. This strain showed, therefore, potential for the production of complete cellulolytic complexes aiming at the saccharification of lignocellulosic materials.

No MeSH data available.


Related in: MedlinePlus