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High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct.

de Castro AM, Ferreira MC, da Cruz JC, Pedro KC, Carvalho DF, Leite SG, Pereira N - Enzyme Res (2010)

Bottom Line: The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol.In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation.The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50°C, accessory xylanase activity, and absence of proteolytic activity towards azocasein.

View Article: PubMed Central - PubMed

Affiliation: Renewable Energy Division, Research and Development Center, Petrobras, Avenue Horácio Macedo, 950 Ilha do Fundão, 21941-915 Rio de Janeiro, RJ, Brazil.

ABSTRACT
The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol. In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation. This fungal strain produced high contents of endoglucanase activity (6,358 U·L(-1)) after 72 hours of process, and further relevant β-glucosidase and FPase activities (742 and 445 U·L(-1), resp.). The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50°C, accessory xylanase activity, and absence of proteolytic activity towards azocasein. This strain showed, therefore, potential for the production of complete cellulolytic complexes aiming at the saccharification of lignocellulosic materials.

No MeSH data available.


Related in: MedlinePlus

Cellulases production by T. harzianum IOC-3844. (-◯-) FPase activity; (-□-)  β-glucosidase activity; (-△-) Endoglucanase activity; (- × -) Protein content.
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Related In: Results  -  Collection


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fig1: Cellulases production by T. harzianum IOC-3844. (-◯-) FPase activity; (-□-)  β-glucosidase activity; (-△-) Endoglucanase activity; (- × -) Protein content.

Mentions: T. harzianum was cultivated in conical flasks by SmF in the presence of pretreated SCB and nutrients in order to induce high-level cellulases production. SCB was tailoredly pretreated in order to generate a high-cellulose-content material (69% glucan) with improved fiber exposition to microbial attack. Recently, when compared to other sugarcane bagasse-derived materials, pretreated SCB induced the best the production of cellulases by Penicillium funiculosum [19]. Figure 1 shows kinetic profiles for the production of three groups of cellulolytic activities, as well as the total extracellular protein content of the extracts, by T. harzianum.


High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct.

de Castro AM, Ferreira MC, da Cruz JC, Pedro KC, Carvalho DF, Leite SG, Pereira N - Enzyme Res (2010)

Cellulases production by T. harzianum IOC-3844. (-◯-) FPase activity; (-□-)  β-glucosidase activity; (-△-) Endoglucanase activity; (- × -) Protein content.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2962913&req=5

fig1: Cellulases production by T. harzianum IOC-3844. (-◯-) FPase activity; (-□-)  β-glucosidase activity; (-△-) Endoglucanase activity; (- × -) Protein content.
Mentions: T. harzianum was cultivated in conical flasks by SmF in the presence of pretreated SCB and nutrients in order to induce high-level cellulases production. SCB was tailoredly pretreated in order to generate a high-cellulose-content material (69% glucan) with improved fiber exposition to microbial attack. Recently, when compared to other sugarcane bagasse-derived materials, pretreated SCB induced the best the production of cellulases by Penicillium funiculosum [19]. Figure 1 shows kinetic profiles for the production of three groups of cellulolytic activities, as well as the total extracellular protein content of the extracts, by T. harzianum.

Bottom Line: The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol.In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation.The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50°C, accessory xylanase activity, and absence of proteolytic activity towards azocasein.

View Article: PubMed Central - PubMed

Affiliation: Renewable Energy Division, Research and Development Center, Petrobras, Avenue Horácio Macedo, 950 Ilha do Fundão, 21941-915 Rio de Janeiro, RJ, Brazil.

ABSTRACT
The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol. In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation. This fungal strain produced high contents of endoglucanase activity (6,358 U·L(-1)) after 72 hours of process, and further relevant β-glucosidase and FPase activities (742 and 445 U·L(-1), resp.). The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50°C, accessory xylanase activity, and absence of proteolytic activity towards azocasein. This strain showed, therefore, potential for the production of complete cellulolytic complexes aiming at the saccharification of lignocellulosic materials.

No MeSH data available.


Related in: MedlinePlus