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Inheritance and identification of molecular markers associated with a novel dwarfing gene in barley.

Ren X, Sun D, Guan W, Sun G, Li C - BMC Genet. (2010)

Bottom Line: All the F1 plants displayed tall trait.Allelism test indicated that this dwarfing gene in the Huaai 11 is nonallelic with the gene br, uzu, sdw1 and denso.Using a double haploid population derived from a cross of Huadamai 6 and Huaai 11 and SSR markers the novel dwarfing gene was mapped onto the long arm of chromosome 7H, and closely linked to Bmac031 and Bmac167 with genetic distance of 2.2 cM.

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Affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT

Background: Dwarfing genes have widely been used in barley breeding program. More than 30 types of dwarfs or semidwarfs have been reported, but a few has been exploited in barley breeding because pleiotropic effects of dwarfing genes cause some undesired traits. The plant architecture of newly discovered dwarfing germplasm "Huaai 11" consisted of desirable agronomic traits such as shortened stature and early maturity. Genetic factor controlling the plant height in dwarf line Huaai 11 was investigated.

Results: The Huaai 11 was crossed with tall varieties Monker, Mpyt, Zhenongda 3, Zaoshu 3, Advance, Huadamai 1, Huadamai 6, Hyproly and Ris01508. All the F1 plants displayed tall trait. Both tall and dwarf plants appeared in all the F2 populations with a 3:1 segregation ratio, suggesting that dwarfism of Huaai 11 is controlled by a single recessive gene, btwd1. Allelism test indicated that this dwarfing gene in the Huaai 11 is nonallelic with the gene br, uzu, sdw1 and denso. Using a double haploid population derived from a cross of Huadamai 6 and Huaai 11 and SSR markers the novel dwarfing gene was mapped onto the long arm of chromosome 7H, and closely linked to Bmac031 and Bmac167 with genetic distance of 2.2 cM.

Conclusion: Huaai 11 is a new source of dwarf for broadening the genetic base of dwarfism. This dwarf source was controlled by a recessive dwarfing gene btwd1, was mapped onto the long arm of chromosome 7H.

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Partial linkage map of chromosome 7H with the dwarfing gene btwd1. The chromosome 7H map was cited from Varshney et al. [35] for comparison
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Figure 3: Partial linkage map of chromosome 7H with the dwarfing gene btwd1. The chromosome 7H map was cited from Varshney et al. [35] for comparison

Mentions: Out of the 306 SSR primers screened, ninety-six are polymorphic between parental lines Huadamai 6 and Huaai 11. The polymorphic SSR markers were used to test the two DNA pools for polymorphism. Four SSR markers, Bmac167, Bmac031, Bmag217 and Bmag900 located on chromosome 7H were polymorphic between the two DNA pools. Polymorphism was further confirmed using the individuals that were used to construct the two DNA pools, suggesting that the gene btwd1 controlling plant height of Huaai 11 was located on chromosome 7H. The four polymorphic markers were used to analyze the 122 individuals of DH population. Linkage analysis between SSR markers and plant height found that the dwarfing gene was located on the long arm of chromosome 7H, associated with the marker Bmac167 and Bmac031 at a genetic distance of 2.2 cM (Figure 3).


Inheritance and identification of molecular markers associated with a novel dwarfing gene in barley.

Ren X, Sun D, Guan W, Sun G, Li C - BMC Genet. (2010)

Partial linkage map of chromosome 7H with the dwarfing gene btwd1. The chromosome 7H map was cited from Varshney et al. [35] for comparison
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2959052&req=5

Figure 3: Partial linkage map of chromosome 7H with the dwarfing gene btwd1. The chromosome 7H map was cited from Varshney et al. [35] for comparison
Mentions: Out of the 306 SSR primers screened, ninety-six are polymorphic between parental lines Huadamai 6 and Huaai 11. The polymorphic SSR markers were used to test the two DNA pools for polymorphism. Four SSR markers, Bmac167, Bmac031, Bmag217 and Bmag900 located on chromosome 7H were polymorphic between the two DNA pools. Polymorphism was further confirmed using the individuals that were used to construct the two DNA pools, suggesting that the gene btwd1 controlling plant height of Huaai 11 was located on chromosome 7H. The four polymorphic markers were used to analyze the 122 individuals of DH population. Linkage analysis between SSR markers and plant height found that the dwarfing gene was located on the long arm of chromosome 7H, associated with the marker Bmac167 and Bmac031 at a genetic distance of 2.2 cM (Figure 3).

Bottom Line: All the F1 plants displayed tall trait.Allelism test indicated that this dwarfing gene in the Huaai 11 is nonallelic with the gene br, uzu, sdw1 and denso.Using a double haploid population derived from a cross of Huadamai 6 and Huaai 11 and SSR markers the novel dwarfing gene was mapped onto the long arm of chromosome 7H, and closely linked to Bmac031 and Bmac167 with genetic distance of 2.2 cM.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT

Background: Dwarfing genes have widely been used in barley breeding program. More than 30 types of dwarfs or semidwarfs have been reported, but a few has been exploited in barley breeding because pleiotropic effects of dwarfing genes cause some undesired traits. The plant architecture of newly discovered dwarfing germplasm "Huaai 11" consisted of desirable agronomic traits such as shortened stature and early maturity. Genetic factor controlling the plant height in dwarf line Huaai 11 was investigated.

Results: The Huaai 11 was crossed with tall varieties Monker, Mpyt, Zhenongda 3, Zaoshu 3, Advance, Huadamai 1, Huadamai 6, Hyproly and Ris01508. All the F1 plants displayed tall trait. Both tall and dwarf plants appeared in all the F2 populations with a 3:1 segregation ratio, suggesting that dwarfism of Huaai 11 is controlled by a single recessive gene, btwd1. Allelism test indicated that this dwarfing gene in the Huaai 11 is nonallelic with the gene br, uzu, sdw1 and denso. Using a double haploid population derived from a cross of Huadamai 6 and Huaai 11 and SSR markers the novel dwarfing gene was mapped onto the long arm of chromosome 7H, and closely linked to Bmac031 and Bmac167 with genetic distance of 2.2 cM.

Conclusion: Huaai 11 is a new source of dwarf for broadening the genetic base of dwarfism. This dwarf source was controlled by a recessive dwarfing gene btwd1, was mapped onto the long arm of chromosome 7H.

Show MeSH
Related in: MedlinePlus