Limits...
E-β-ocimene, a volatile brood pheromone involved in social regulation in the honey bee colony (Apis mellifera).

Maisonnasse A, Lenoir JC, Beslay D, Crauser D, Le Conte Y - PLoS ONE (2010)

Bottom Line: A brood ester pheromone produced primarily by old larvae (4 and 5 days old larvae) was first identified as acting as a contact pheromone with specific effects on nurses in the colony.In this way, young larvae are able to assign precedence to the task of foraging by workers in order to increase food stores for their own development.Thus, in the complexity of honey bee chemical communication, E-β-ocimene, a pheromone of young larvae, provides the brood with the means to express their nutritional needs to the workers.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR 406, Abeilles et Environnement, Laboratoire Biologie et Protection de l'Abeille, Avignon, France. alban.maisonnasse@avignon.inra.fr

ABSTRACT

Background: In honey bee colony, the brood is able to manipulate and chemically control the workers in order to sustain their own development. A brood ester pheromone produced primarily by old larvae (4 and 5 days old larvae) was first identified as acting as a contact pheromone with specific effects on nurses in the colony. More recently a new volatile brood pheromone has been identified: E-β-ocimene, which partially inhibits ovary development in workers.

Methodology and principal finding: Our analysis of E-β-ocimene production revealed that young brood (newly hatched to 3 days old) produce the highest quantity of E-β-ocimene relative to their body weight. By testing the potential action of this molecule as a non-specific larval signal, due to its high volatility in the colony, we demonstrated that in the presence of E-β-ocimene nest workers start to forage earlier in life, as seen in the presence of real brood.

Conclusions/significance: In this way, young larvae are able to assign precedence to the task of foraging by workers in order to increase food stores for their own development. Thus, in the complexity of honey bee chemical communication, E-β-ocimene, a pheromone of young larvae, provides the brood with the means to express their nutritional needs to the workers.

Show MeSH

Related in: MedlinePlus

Production of BEP (red) and E-β-ocimene (blue) per mg of the different brood instars and their effects on worker physiology and behaviour.(L1 to L5, pre-pupae PP, pupae white eyes WE, pink eyes PE, black eyes BE).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2958837&req=5

pone-0013531-g003: Production of BEP (red) and E-β-ocimene (blue) per mg of the different brood instars and their effects on worker physiology and behaviour.(L1 to L5, pre-pupae PP, pupae white eyes WE, pink eyes PE, black eyes BE).

Mentions: At individual level the young and the old larvae emit different quantities of pheromones that have different volatilities. Taken as the amount of compound produced per gram of larvae, E-β-ocimene is emitted principally by the young instars (L1, L2-3) while BEP reaches a maximum value during the capping stage (L4-5) [19] (Fig. 3). E-β-ocimene (boiling point 73°C), which belongs to the terpene family, is volatile so it has an aerial transmission (targeting all worker castes), while the BEP (boiling point around 200°C), which belongs to the ester family, has a low volatility which is transmitted by contact (target workers close to the larvae cells).


E-β-ocimene, a volatile brood pheromone involved in social regulation in the honey bee colony (Apis mellifera).

Maisonnasse A, Lenoir JC, Beslay D, Crauser D, Le Conte Y - PLoS ONE (2010)

Production of BEP (red) and E-β-ocimene (blue) per mg of the different brood instars and their effects on worker physiology and behaviour.(L1 to L5, pre-pupae PP, pupae white eyes WE, pink eyes PE, black eyes BE).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2958837&req=5

pone-0013531-g003: Production of BEP (red) and E-β-ocimene (blue) per mg of the different brood instars and their effects on worker physiology and behaviour.(L1 to L5, pre-pupae PP, pupae white eyes WE, pink eyes PE, black eyes BE).
Mentions: At individual level the young and the old larvae emit different quantities of pheromones that have different volatilities. Taken as the amount of compound produced per gram of larvae, E-β-ocimene is emitted principally by the young instars (L1, L2-3) while BEP reaches a maximum value during the capping stage (L4-5) [19] (Fig. 3). E-β-ocimene (boiling point 73°C), which belongs to the terpene family, is volatile so it has an aerial transmission (targeting all worker castes), while the BEP (boiling point around 200°C), which belongs to the ester family, has a low volatility which is transmitted by contact (target workers close to the larvae cells).

Bottom Line: A brood ester pheromone produced primarily by old larvae (4 and 5 days old larvae) was first identified as acting as a contact pheromone with specific effects on nurses in the colony.In this way, young larvae are able to assign precedence to the task of foraging by workers in order to increase food stores for their own development.Thus, in the complexity of honey bee chemical communication, E-β-ocimene, a pheromone of young larvae, provides the brood with the means to express their nutritional needs to the workers.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR 406, Abeilles et Environnement, Laboratoire Biologie et Protection de l'Abeille, Avignon, France. alban.maisonnasse@avignon.inra.fr

ABSTRACT

Background: In honey bee colony, the brood is able to manipulate and chemically control the workers in order to sustain their own development. A brood ester pheromone produced primarily by old larvae (4 and 5 days old larvae) was first identified as acting as a contact pheromone with specific effects on nurses in the colony. More recently a new volatile brood pheromone has been identified: E-β-ocimene, which partially inhibits ovary development in workers.

Methodology and principal finding: Our analysis of E-β-ocimene production revealed that young brood (newly hatched to 3 days old) produce the highest quantity of E-β-ocimene relative to their body weight. By testing the potential action of this molecule as a non-specific larval signal, due to its high volatility in the colony, we demonstrated that in the presence of E-β-ocimene nest workers start to forage earlier in life, as seen in the presence of real brood.

Conclusions/significance: In this way, young larvae are able to assign precedence to the task of foraging by workers in order to increase food stores for their own development. Thus, in the complexity of honey bee chemical communication, E-β-ocimene, a pheromone of young larvae, provides the brood with the means to express their nutritional needs to the workers.

Show MeSH
Related in: MedlinePlus