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Inhibitory Effects of Terminalia catappa on UVB-Induced Photodamage in Fibroblast Cell Line.

Wen KC, Shih IC, Hu JC, Liao ST, Su TW, Chiang HM - Evid Based Complement Alternat Med (2010)

Bottom Line: We found that serial dilutions of TCLW (10-500 μg/mL) inhibited collagenase activity in a dose-dependent manner (82.3% to 101.0%).However, TCLW did not significantly inhibit elastase activity.We also found that TCLW attenuated the expression of MMP-1, -3, and -9 by inhibiting the phosphorylation of ERK, JNK, and p38.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan.

ABSTRACT
This study investigated whether Terminalia catappa L. hydrophilic extract (TCLW) prevents photoaging in human dermal fibroblasts after exposure to UVB radiation. TCLW exhibited DPPH free radical scavenging activity and protected erythrocytes against AAPH-induced hemolysis. In the gelatin digestion assay, the rates of collagenase inhibition by TCL methanol extract, TCLW, and its hydrolysates were greater than 100% at the concentration of 1 mg/mL. We found that serial dilutions of TCLW (10-500 μg/mL) inhibited collagenase activity in a dose-dependent manner (82.3% to 101.0%). However, TCLW did not significantly inhibit elastase activity. In addition, TCLW inhibited MMP-1 and MMP-9 protein expression at a concentration of 25 μg/mL and inhibited MMP-3 protein expression at a concentration of 50 μg/mL. TCLW also promoted the protein expression of type I procollagen. We also found that TCLW attenuated the expression of MMP-1, -3, and -9 by inhibiting the phosphorylation of ERK, JNK, and p38. These findings suggest that TCLW increases the production of type I procollagen by inhibiting the activity of MMP-1, -3 and -9, and, therefore, has potential use in anti-aging cosmetics.

No MeSH data available.


Related in: MedlinePlus

Cell viability of TCLW on human fibroblasts. (n = 4; **P < .01; ***P < .001).
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fig8: Cell viability of TCLW on human fibroblasts. (n = 4; **P < .01; ***P < .001).

Mentions: Hs68 cells were treated with various concentrations of TCLW (5–200 μg/mL) and cell viability was measured. As shown in Figure 8, TCLW did not exhibit cytotoxic effects on the proliferation of cells (cell viability >90% of control). In addition, TCLW promoted cell proliferation in a dose-dependent manner.


Inhibitory Effects of Terminalia catappa on UVB-Induced Photodamage in Fibroblast Cell Line.

Wen KC, Shih IC, Hu JC, Liao ST, Su TW, Chiang HM - Evid Based Complement Alternat Med (2010)

Cell viability of TCLW on human fibroblasts. (n = 4; **P < .01; ***P < .001).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2958628&req=5

fig8: Cell viability of TCLW on human fibroblasts. (n = 4; **P < .01; ***P < .001).
Mentions: Hs68 cells were treated with various concentrations of TCLW (5–200 μg/mL) and cell viability was measured. As shown in Figure 8, TCLW did not exhibit cytotoxic effects on the proliferation of cells (cell viability >90% of control). In addition, TCLW promoted cell proliferation in a dose-dependent manner.

Bottom Line: We found that serial dilutions of TCLW (10-500 μg/mL) inhibited collagenase activity in a dose-dependent manner (82.3% to 101.0%).However, TCLW did not significantly inhibit elastase activity.We also found that TCLW attenuated the expression of MMP-1, -3, and -9 by inhibiting the phosphorylation of ERK, JNK, and p38.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan.

ABSTRACT
This study investigated whether Terminalia catappa L. hydrophilic extract (TCLW) prevents photoaging in human dermal fibroblasts after exposure to UVB radiation. TCLW exhibited DPPH free radical scavenging activity and protected erythrocytes against AAPH-induced hemolysis. In the gelatin digestion assay, the rates of collagenase inhibition by TCL methanol extract, TCLW, and its hydrolysates were greater than 100% at the concentration of 1 mg/mL. We found that serial dilutions of TCLW (10-500 μg/mL) inhibited collagenase activity in a dose-dependent manner (82.3% to 101.0%). However, TCLW did not significantly inhibit elastase activity. In addition, TCLW inhibited MMP-1 and MMP-9 protein expression at a concentration of 25 μg/mL and inhibited MMP-3 protein expression at a concentration of 50 μg/mL. TCLW also promoted the protein expression of type I procollagen. We also found that TCLW attenuated the expression of MMP-1, -3, and -9 by inhibiting the phosphorylation of ERK, JNK, and p38. These findings suggest that TCLW increases the production of type I procollagen by inhibiting the activity of MMP-1, -3 and -9, and, therefore, has potential use in anti-aging cosmetics.

No MeSH data available.


Related in: MedlinePlus