Limits...
The effect of pro-inflammatory cytokines on the discharge rate of vagal nerve paraganglia in the rat.

Mac Grory B, O'Connor ET, O'Halloran KD, Jones JF - Respir Physiol Neurobiol (2010)

Bottom Line: Twenty-three successful single fibre recordings were obtained from 10 animals.IL-1 beta and TNF-alpha had no statistically significant effect on the frequency of action potentials observed (p=0.39 and 0.42, respectively, repeated measures ANOVA).In conclusion, an immune role for SLN paraganglia has not been established.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine and Medical Science, Health Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland.

Show MeSH

Related in: MedlinePlus

Effect of cytokines on translocation of P65-NFκB to the nuclei of HELA cells. Upper panels. On the left are shown control HELA cells that display a cytoplasmic location of P65-NFκB. Following 30 min exposure to TNF-α (10 ng/ml) there is an obvious translocation of P65-NFκB (green signal) to the nucleus (stained with Hoescht blue). Scale bar = 50 μm. Lower panels. Untreated control cells are shown on the left and the right panel shows HELA cells that were incubated with 5 ng/ml IL-1β for 30 min. The arrows indicate cells that have nuclear localization of P65-NFκB. The intense small dots are within the nuclei and may indicate the position of the nucleoli which are targets for P65-NFκB. Scale bar = 100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2958315&req=5

fig3: Effect of cytokines on translocation of P65-NFκB to the nuclei of HELA cells. Upper panels. On the left are shown control HELA cells that display a cytoplasmic location of P65-NFκB. Following 30 min exposure to TNF-α (10 ng/ml) there is an obvious translocation of P65-NFκB (green signal) to the nucleus (stained with Hoescht blue). Scale bar = 50 μm. Lower panels. Untreated control cells are shown on the left and the right panel shows HELA cells that were incubated with 5 ng/ml IL-1β for 30 min. The arrows indicate cells that have nuclear localization of P65-NFκB. The intense small dots are within the nuclei and may indicate the position of the nucleoli which are targets for P65-NFκB. Scale bar = 100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)

Mentions: Both cytokines produced the expected translocation of P65-NFκB from cytoplasm to nucleus in HELA cells (Fig. 3) when dissolved in the solutions used in our experiments.


The effect of pro-inflammatory cytokines on the discharge rate of vagal nerve paraganglia in the rat.

Mac Grory B, O'Connor ET, O'Halloran KD, Jones JF - Respir Physiol Neurobiol (2010)

Effect of cytokines on translocation of P65-NFκB to the nuclei of HELA cells. Upper panels. On the left are shown control HELA cells that display a cytoplasmic location of P65-NFκB. Following 30 min exposure to TNF-α (10 ng/ml) there is an obvious translocation of P65-NFκB (green signal) to the nucleus (stained with Hoescht blue). Scale bar = 50 μm. Lower panels. Untreated control cells are shown on the left and the right panel shows HELA cells that were incubated with 5 ng/ml IL-1β for 30 min. The arrows indicate cells that have nuclear localization of P65-NFκB. The intense small dots are within the nuclei and may indicate the position of the nucleoli which are targets for P65-NFκB. Scale bar = 100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2958315&req=5

fig3: Effect of cytokines on translocation of P65-NFκB to the nuclei of HELA cells. Upper panels. On the left are shown control HELA cells that display a cytoplasmic location of P65-NFκB. Following 30 min exposure to TNF-α (10 ng/ml) there is an obvious translocation of P65-NFκB (green signal) to the nucleus (stained with Hoescht blue). Scale bar = 50 μm. Lower panels. Untreated control cells are shown on the left and the right panel shows HELA cells that were incubated with 5 ng/ml IL-1β for 30 min. The arrows indicate cells that have nuclear localization of P65-NFκB. The intense small dots are within the nuclei and may indicate the position of the nucleoli which are targets for P65-NFκB. Scale bar = 100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)
Mentions: Both cytokines produced the expected translocation of P65-NFκB from cytoplasm to nucleus in HELA cells (Fig. 3) when dissolved in the solutions used in our experiments.

Bottom Line: Twenty-three successful single fibre recordings were obtained from 10 animals.IL-1 beta and TNF-alpha had no statistically significant effect on the frequency of action potentials observed (p=0.39 and 0.42, respectively, repeated measures ANOVA).In conclusion, an immune role for SLN paraganglia has not been established.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine and Medical Science, Health Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland.

Show MeSH
Related in: MedlinePlus