Studies on the morphology and spreading of human endothelial cells define key inter- and intramolecular interactions for talin1.
Bottom Line: The talin rod contains several actin-binding sites (ABS), and mutations in the C-terminal ABS that reduced actin-binding impaired talin1 function, whereas those that increased binding resulted in more stable FAs.The results show that both the N-terminal integrin and C-terminal actin-binding functions of talin are essential to cell spreading and FA assembly.Finally, mutations that relieve talin auto-inhibition resulted in the rapid and excessive production of FA, highlighting the importance of talin regulation within the cell.
Affiliation: Department of Biochemistry, University of Leicester, Leicester, UK.Show MeSH
Related in: MedlinePlus
Affiliation: Department of Biochemistry, University of Leicester, Leicester, UK.