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Novel functional aspect of antihistamines: the impact of bepotastine besilate on substance p-induced events.

Kitaba S, Murota H, Yahata Y, Azukizawa H, Katayama I - J Allergy (Cairo) (2009)

Bottom Line: Besides histamine, substance P (SP) has been demonstrated to play a crucial role in pruritic skin diseases.Our aim was to study the effect of bepotastine besilate on SP-induced degranulation of rat basophillic leukemia (RBL-2H3) cells and expression of adhesion molecules and NO synthesis in human dermal microvascular endothelial cells (HMVECs).Bepotastine besilate significantly inhibited SP-induced degranulation of RBL-2H3 cells and NO synthesis in HMVECs.

View Article: PubMed Central - PubMed

Affiliation: Course of Integrated Medicine, Department of Dermatology, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita-Shi, Osaka 565-0871, Japan.

ABSTRACT
Besides histamine, substance P (SP) has been demonstrated to play a crucial role in pruritic skin diseases. Although antihistamines are frequently used for pruritic skin diseases, little is known concerning the effect on an SP-induced event such as mast cell degranulation and the upregulation of adhesion molecules or the nitric oxide (NO) synthesis in endothelial cells. Our aim was to study the effect of bepotastine besilate on SP-induced degranulation of rat basophillic leukemia (RBL-2H3) cells and expression of adhesion molecules and NO synthesis in human dermal microvascular endothelial cells (HMVECs). Bepotastine besilate significantly inhibited SP-induced degranulation of RBL-2H3 cells and NO synthesis in HMVECs. Bepotastine besilate significantly inhibited expression of adhesion molecules in HMVESs, while it failed to suppress SP-induced upregulation of the adhesion molecules in HMVECs. Therefore, bepotastine besilate is assumed to act favorably on SP-induced basophil degranulation and NO synthesis in HMVECs.

No MeSH data available.


Related in: MedlinePlus

Results of the β-hexosaminidase release assay. Control; mock treated. SP: substance P. Error bar: standard deviation (SD). N = 3–5.
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fig1: Results of the β-hexosaminidase release assay. Control; mock treated. SP: substance P. Error bar: standard deviation (SD). N = 3–5.

Mentions: To investigate the effect of antihistamines on the SP-induced basophil degranulation, the concentration of released β-hexosaminidase was measured (Figure 1). As expected, 1 × 10−4 M of SP-induced the release of β-hexosaminidase, while 1 × 10−6 M of SP failed to induce the release. Interestingly, bepotastine besilate significantly suppressed the SP-mediated release of β-hexosaminidase (P = .0009, unpaired t-test, 1 × 10−4 M of SP versus 1 × 10−4 M of SP + bepotastine), while pyliramine failed to suppress. This result suggests that bepotastine besilate potentially affects substance P signaling.


Novel functional aspect of antihistamines: the impact of bepotastine besilate on substance p-induced events.

Kitaba S, Murota H, Yahata Y, Azukizawa H, Katayama I - J Allergy (Cairo) (2009)

Results of the β-hexosaminidase release assay. Control; mock treated. SP: substance P. Error bar: standard deviation (SD). N = 3–5.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2958303&req=5

fig1: Results of the β-hexosaminidase release assay. Control; mock treated. SP: substance P. Error bar: standard deviation (SD). N = 3–5.
Mentions: To investigate the effect of antihistamines on the SP-induced basophil degranulation, the concentration of released β-hexosaminidase was measured (Figure 1). As expected, 1 × 10−4 M of SP-induced the release of β-hexosaminidase, while 1 × 10−6 M of SP failed to induce the release. Interestingly, bepotastine besilate significantly suppressed the SP-mediated release of β-hexosaminidase (P = .0009, unpaired t-test, 1 × 10−4 M of SP versus 1 × 10−4 M of SP + bepotastine), while pyliramine failed to suppress. This result suggests that bepotastine besilate potentially affects substance P signaling.

Bottom Line: Besides histamine, substance P (SP) has been demonstrated to play a crucial role in pruritic skin diseases.Our aim was to study the effect of bepotastine besilate on SP-induced degranulation of rat basophillic leukemia (RBL-2H3) cells and expression of adhesion molecules and NO synthesis in human dermal microvascular endothelial cells (HMVECs).Bepotastine besilate significantly inhibited SP-induced degranulation of RBL-2H3 cells and NO synthesis in HMVECs.

View Article: PubMed Central - PubMed

Affiliation: Course of Integrated Medicine, Department of Dermatology, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita-Shi, Osaka 565-0871, Japan.

ABSTRACT
Besides histamine, substance P (SP) has been demonstrated to play a crucial role in pruritic skin diseases. Although antihistamines are frequently used for pruritic skin diseases, little is known concerning the effect on an SP-induced event such as mast cell degranulation and the upregulation of adhesion molecules or the nitric oxide (NO) synthesis in endothelial cells. Our aim was to study the effect of bepotastine besilate on SP-induced degranulation of rat basophillic leukemia (RBL-2H3) cells and expression of adhesion molecules and NO synthesis in human dermal microvascular endothelial cells (HMVECs). Bepotastine besilate significantly inhibited SP-induced degranulation of RBL-2H3 cells and NO synthesis in HMVECs. Bepotastine besilate significantly inhibited expression of adhesion molecules in HMVESs, while it failed to suppress SP-induced upregulation of the adhesion molecules in HMVECs. Therefore, bepotastine besilate is assumed to act favorably on SP-induced basophil degranulation and NO synthesis in HMVECs.

No MeSH data available.


Related in: MedlinePlus