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Marburg virus in fruit bat, Kenya.

Kuzmin IV, Niezgoda M, Franka R, Agwanda B, Markotter W, Breiman RF, Shieh WJ, Zaki SR, Rupprecht CE - Emerging Infect. Dis. (2010)

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Thereafter, MARV was reported throughout sub-Saharan Africa... Most outbreaks in humans were associated with visits to caves and mines... MARV RNA was detected in pooled liver, spleen, and lung tissue of an apparently healthy, pregnant, female R. aegyptiacus bat obtained at Kitum Cave in July 2007 (Figure)... Tissues of other bats, including 75 R. aegyptiacus (29 pregnant females) from Kitum Cave and neighboring Makingeni Cave, were negative for MARV RNA... Our results are similar to those reported from Gabon and the DRC, where MARV RNA was detected in tissues of 1.4% and 3.1% of R. aegyptiacus bats, respectively, with negative isolation attempts... In contrast, seroprevalence of MARV-neutralizing antibodies in colonies of R. aegyptiacus bats in which PCR-positive bats were collected was only 12% or as low as 2.4%... This low seroprevalence may be interpreted as a result of a limited spillover of MARV into bats from another source... The association of human cases of MARV with visiting caves often inhabited by R. aegyptiacus and other bat species is obvious... This association was reinforced by MARV infection in tourists who visited caves in Uganda... For Kenya, our finding is consistent with reported human cases tentatively associated with visiting of Kitum Cave... We do not know if MARV has persisted in this area continuously or has reemerged sporadically... Scale bar indicates nucleotide substitutions per site... DRC, Democratic Republic of Congo.

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Bat collection sites (open circles) and location of Kitum Cave, Kenya, where Lake Victoria Marburgvirus was detected (solid circle).
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Figure 1: Bat collection sites (open circles) and location of Kitum Cave, Kenya, where Lake Victoria Marburgvirus was detected (solid circle).

Mentions: MARV RNA was detected in pooled liver, spleen, and lung tissue of an apparently healthy, pregnant, female R. aegyptiacus bat obtained at Kitum Cave in July 2007 (Figure). A faint band was obtained only after nested RT-PCR, which suggests that the RNA load was limited. Attempts at virus isolation were not performed. Phylogenetic comparisons demonstrated that the virus (KE261, GenBank accession no. GQ499199) was relatively distant from previous isolates from Kenya (Musoke and Ravn). It was similar to viruses isolated from index cases in Europe in 1967 (Popp and Ci67). This lineage also contained virus 02DRC99, which was isolated from a human in the DRC in 1999 (Appendix Figure). MARV isolates obtained from bats and humans in Uganda in 2007 belong to distinct lineages (6) (Appendix Figure). Tissues of other bats, including 75 R. aegyptiacus (29 pregnant females) from Kitum Cave and neighboring Makingeni Cave, were negative for MARV RNA.


Marburg virus in fruit bat, Kenya.

Kuzmin IV, Niezgoda M, Franka R, Agwanda B, Markotter W, Breiman RF, Shieh WJ, Zaki SR, Rupprecht CE - Emerging Infect. Dis. (2010)

Bat collection sites (open circles) and location of Kitum Cave, Kenya, where Lake Victoria Marburgvirus was detected (solid circle).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2958024&req=5

Figure 1: Bat collection sites (open circles) and location of Kitum Cave, Kenya, where Lake Victoria Marburgvirus was detected (solid circle).
Mentions: MARV RNA was detected in pooled liver, spleen, and lung tissue of an apparently healthy, pregnant, female R. aegyptiacus bat obtained at Kitum Cave in July 2007 (Figure). A faint band was obtained only after nested RT-PCR, which suggests that the RNA load was limited. Attempts at virus isolation were not performed. Phylogenetic comparisons demonstrated that the virus (KE261, GenBank accession no. GQ499199) was relatively distant from previous isolates from Kenya (Musoke and Ravn). It was similar to viruses isolated from index cases in Europe in 1967 (Popp and Ci67). This lineage also contained virus 02DRC99, which was isolated from a human in the DRC in 1999 (Appendix Figure). MARV isolates obtained from bats and humans in Uganda in 2007 belong to distinct lineages (6) (Appendix Figure). Tissues of other bats, including 75 R. aegyptiacus (29 pregnant females) from Kitum Cave and neighboring Makingeni Cave, were negative for MARV RNA.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Thereafter, MARV was reported throughout sub-Saharan Africa... Most outbreaks in humans were associated with visits to caves and mines... MARV RNA was detected in pooled liver, spleen, and lung tissue of an apparently healthy, pregnant, female R. aegyptiacus bat obtained at Kitum Cave in July 2007 (Figure)... Tissues of other bats, including 75 R. aegyptiacus (29 pregnant females) from Kitum Cave and neighboring Makingeni Cave, were negative for MARV RNA... Our results are similar to those reported from Gabon and the DRC, where MARV RNA was detected in tissues of 1.4% and 3.1% of R. aegyptiacus bats, respectively, with negative isolation attempts... In contrast, seroprevalence of MARV-neutralizing antibodies in colonies of R. aegyptiacus bats in which PCR-positive bats were collected was only 12% or as low as 2.4%... This low seroprevalence may be interpreted as a result of a limited spillover of MARV into bats from another source... The association of human cases of MARV with visiting caves often inhabited by R. aegyptiacus and other bat species is obvious... This association was reinforced by MARV infection in tourists who visited caves in Uganda... For Kenya, our finding is consistent with reported human cases tentatively associated with visiting of Kitum Cave... We do not know if MARV has persisted in this area continuously or has reemerged sporadically... Scale bar indicates nucleotide substitutions per site... DRC, Democratic Republic of Congo.

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