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Hemorrhagic fever with renal syndrome, Vietnam.

Huong VT, Yoshimatsu K, Luan VD, Tuan le V, Nhi L, Arikawa J, Nguyen TM - Emerging Infect. Dis. (2010)

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: Hantaviruses are primarily rodent borne and can cause hemorrhagic fever with renal syndrome (HFRS) in persons who inhale aerosolized excreta from infected rodents... The clinical characteristics of HFRS are fever, hemorrhage, and varying degrees of renal and hepatic dysfunction... Although HFRS is endemic primarily to Eurasian regions, there is serologic evidence of hantavirus infections in rodents and humans worldwide... Little is known about the occurrence of hantavirus infection in rodents or humans in Vietnam... One study found 5.4% prevalence of antibodies against Hantaan 76–118 and Puumala strains among residents of the Hanoi Metropolitan, whereas another study in southern Vietnam did not find evidence of hantavirus infection in humans... The presence of antihantavirus IgG was confirmed by immunofluorescent antibody (IFA) assay using whole hantavirus antigen and Western blot using hantavirus CL-1 strain... In further analysis, neutralization antibodies against SEOV strain SR-11 were detected by focus reduction neutralization test... The viral RNA, however, was not detectable in the acute-phase blood sample by reverse transcription–PCR (RT-PCR)... Other serologic tests were performed for dengue fever, typhoid fever, hepatitis B, and malaria; results of culture of blood and urine for bacteria were negative... Further analysis using RT-PCR identified 2 SEOV strains from R. norvegicus and R. argentiventer captured in the patient’s house... The M segment of 1 identified SEOV strain (24D1208) was sequenced and compared with 22 SEOV strains, 6 of which were from R. norvegicus rats captured in urban areas of North Vietnam... Our epidemiologic findings were compatible with other studies indicating the source of infection was the case-patient’s home, the only place where she had a history of exposure to rodents... Although viral RNA could not be obtained from the case-patient for genotyping, the genomic comparison of the viral strains from rodents captured in the case-patient’s home and elsewhere in Vietnam suggested that the source of infection was local rodents... This report provides additional evidence that hantavirus infection is a worldwide problem and is likely underdiagnosed in Vietnam and other countries where simple standardized laboratory diagnostics are not widely available.

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Phylogenetic tree (CLC-Combined Workbench 3) showing partial sequences of the medium segment (nt 810–2355). The newly identified Seoul virus (SEOV) was denoted as 24D1208 (arrow). The M segment sequences of the reference strains are: SEOV strains KI-88-15 (D17594), KI-85-1 (D17593), KI-83-262 (D17592), SR11 (M34882), 80–39 (S47716), Jakarta137 (AJ620583), Haiphong port #7 (AB355728), Haiphong port #20 (AB355730), Haiphong port #16 (AB355729), Hanoi #25 (AB355733), Hanoi #9 (AB355732), Haiphong port #28 (AB355731), B-1 (X53861), BjHD01 (DQ133505), ZT71 (EF117248), ZT10 (DQ159911), K24-e7 (AF288652), K24-v2 (AF288654), HB55 (AF035832), IR461 (AF458104), Gou3-e5 (AF288650), and ZJ5 (FJ811839); Thailand virus strain 749 (L08756); Hantaan virus strains 76–118 (M14627), Hantaan (NC005219), LEE (D00377) and Hojo (D00376); Dobrava virus (DOBV) strain Dobrava (L33685); Puumala virus strain Sotkamo (X61034); Tula virus (TUV) strain Tula/Moravia/5302v/95 (Z69993); and Sin Nombre virus (SNV) strain NMH10. The numbers at the nodes are bootstrap confidence levels for 1,000 replications. Only bootstrap support values >70% are shown.
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Figure 1: Phylogenetic tree (CLC-Combined Workbench 3) showing partial sequences of the medium segment (nt 810–2355). The newly identified Seoul virus (SEOV) was denoted as 24D1208 (arrow). The M segment sequences of the reference strains are: SEOV strains KI-88-15 (D17594), KI-85-1 (D17593), KI-83-262 (D17592), SR11 (M34882), 80–39 (S47716), Jakarta137 (AJ620583), Haiphong port #7 (AB355728), Haiphong port #20 (AB355730), Haiphong port #16 (AB355729), Hanoi #25 (AB355733), Hanoi #9 (AB355732), Haiphong port #28 (AB355731), B-1 (X53861), BjHD01 (DQ133505), ZT71 (EF117248), ZT10 (DQ159911), K24-e7 (AF288652), K24-v2 (AF288654), HB55 (AF035832), IR461 (AF458104), Gou3-e5 (AF288650), and ZJ5 (FJ811839); Thailand virus strain 749 (L08756); Hantaan virus strains 76–118 (M14627), Hantaan (NC005219), LEE (D00377) and Hojo (D00376); Dobrava virus (DOBV) strain Dobrava (L33685); Puumala virus strain Sotkamo (X61034); Tula virus (TUV) strain Tula/Moravia/5302v/95 (Z69993); and Sin Nombre virus (SNV) strain NMH10. The numbers at the nodes are bootstrap confidence levels for 1,000 replications. Only bootstrap support values >70% are shown.

Mentions: From October 14 through 16, 2008, 110 rodent traps were set within and surrounding the patient’s house. The total catch was 32 rodents, of which 16 were Rattus norvegicus, 7 R. exulans, 5 R. argentiventer, and 4 Bandicota indica. By using ELISA, IFA, and Western blot, antihantavirus IgG was detected in serum from 7 rats, of which 5 were R. norvegicus, 1 R. argentiventer, and 1 B. indica. Further analysis using RT-PCR identified 2 SEOV strains from R. norvegicus and R. argentiventer captured in the patient’s house. The M segment of 1 identified SEOV strain (24D1208) was sequenced and compared with 22 SEOV strains, 6 of which were from R. norvegicus rats captured in urban areas of North Vietnam. Phylogenetic analysis showed that this SEOV belonged to the Vietnamese SEOV genotype (Figure).


Hemorrhagic fever with renal syndrome, Vietnam.

Huong VT, Yoshimatsu K, Luan VD, Tuan le V, Nhi L, Arikawa J, Nguyen TM - Emerging Infect. Dis. (2010)

Phylogenetic tree (CLC-Combined Workbench 3) showing partial sequences of the medium segment (nt 810–2355). The newly identified Seoul virus (SEOV) was denoted as 24D1208 (arrow). The M segment sequences of the reference strains are: SEOV strains KI-88-15 (D17594), KI-85-1 (D17593), KI-83-262 (D17592), SR11 (M34882), 80–39 (S47716), Jakarta137 (AJ620583), Haiphong port #7 (AB355728), Haiphong port #20 (AB355730), Haiphong port #16 (AB355729), Hanoi #25 (AB355733), Hanoi #9 (AB355732), Haiphong port #28 (AB355731), B-1 (X53861), BjHD01 (DQ133505), ZT71 (EF117248), ZT10 (DQ159911), K24-e7 (AF288652), K24-v2 (AF288654), HB55 (AF035832), IR461 (AF458104), Gou3-e5 (AF288650), and ZJ5 (FJ811839); Thailand virus strain 749 (L08756); Hantaan virus strains 76–118 (M14627), Hantaan (NC005219), LEE (D00377) and Hojo (D00376); Dobrava virus (DOBV) strain Dobrava (L33685); Puumala virus strain Sotkamo (X61034); Tula virus (TUV) strain Tula/Moravia/5302v/95 (Z69993); and Sin Nombre virus (SNV) strain NMH10. The numbers at the nodes are bootstrap confidence levels for 1,000 replications. Only bootstrap support values >70% are shown.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2958020&req=5

Figure 1: Phylogenetic tree (CLC-Combined Workbench 3) showing partial sequences of the medium segment (nt 810–2355). The newly identified Seoul virus (SEOV) was denoted as 24D1208 (arrow). The M segment sequences of the reference strains are: SEOV strains KI-88-15 (D17594), KI-85-1 (D17593), KI-83-262 (D17592), SR11 (M34882), 80–39 (S47716), Jakarta137 (AJ620583), Haiphong port #7 (AB355728), Haiphong port #20 (AB355730), Haiphong port #16 (AB355729), Hanoi #25 (AB355733), Hanoi #9 (AB355732), Haiphong port #28 (AB355731), B-1 (X53861), BjHD01 (DQ133505), ZT71 (EF117248), ZT10 (DQ159911), K24-e7 (AF288652), K24-v2 (AF288654), HB55 (AF035832), IR461 (AF458104), Gou3-e5 (AF288650), and ZJ5 (FJ811839); Thailand virus strain 749 (L08756); Hantaan virus strains 76–118 (M14627), Hantaan (NC005219), LEE (D00377) and Hojo (D00376); Dobrava virus (DOBV) strain Dobrava (L33685); Puumala virus strain Sotkamo (X61034); Tula virus (TUV) strain Tula/Moravia/5302v/95 (Z69993); and Sin Nombre virus (SNV) strain NMH10. The numbers at the nodes are bootstrap confidence levels for 1,000 replications. Only bootstrap support values >70% are shown.
Mentions: From October 14 through 16, 2008, 110 rodent traps were set within and surrounding the patient’s house. The total catch was 32 rodents, of which 16 were Rattus norvegicus, 7 R. exulans, 5 R. argentiventer, and 4 Bandicota indica. By using ELISA, IFA, and Western blot, antihantavirus IgG was detected in serum from 7 rats, of which 5 were R. norvegicus, 1 R. argentiventer, and 1 B. indica. Further analysis using RT-PCR identified 2 SEOV strains from R. norvegicus and R. argentiventer captured in the patient’s house. The M segment of 1 identified SEOV strain (24D1208) was sequenced and compared with 22 SEOV strains, 6 of which were from R. norvegicus rats captured in urban areas of North Vietnam. Phylogenetic analysis showed that this SEOV belonged to the Vietnamese SEOV genotype (Figure).

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

: Hantaviruses are primarily rodent borne and can cause hemorrhagic fever with renal syndrome (HFRS) in persons who inhale aerosolized excreta from infected rodents... The clinical characteristics of HFRS are fever, hemorrhage, and varying degrees of renal and hepatic dysfunction... Although HFRS is endemic primarily to Eurasian regions, there is serologic evidence of hantavirus infections in rodents and humans worldwide... Little is known about the occurrence of hantavirus infection in rodents or humans in Vietnam... One study found 5.4% prevalence of antibodies against Hantaan 76–118 and Puumala strains among residents of the Hanoi Metropolitan, whereas another study in southern Vietnam did not find evidence of hantavirus infection in humans... The presence of antihantavirus IgG was confirmed by immunofluorescent antibody (IFA) assay using whole hantavirus antigen and Western blot using hantavirus CL-1 strain... In further analysis, neutralization antibodies against SEOV strain SR-11 were detected by focus reduction neutralization test... The viral RNA, however, was not detectable in the acute-phase blood sample by reverse transcription–PCR (RT-PCR)... Other serologic tests were performed for dengue fever, typhoid fever, hepatitis B, and malaria; results of culture of blood and urine for bacteria were negative... Further analysis using RT-PCR identified 2 SEOV strains from R. norvegicus and R. argentiventer captured in the patient’s house... The M segment of 1 identified SEOV strain (24D1208) was sequenced and compared with 22 SEOV strains, 6 of which were from R. norvegicus rats captured in urban areas of North Vietnam... Our epidemiologic findings were compatible with other studies indicating the source of infection was the case-patient’s home, the only place where she had a history of exposure to rodents... Although viral RNA could not be obtained from the case-patient for genotyping, the genomic comparison of the viral strains from rodents captured in the case-patient’s home and elsewhere in Vietnam suggested that the source of infection was local rodents... This report provides additional evidence that hantavirus infection is a worldwide problem and is likely underdiagnosed in Vietnam and other countries where simple standardized laboratory diagnostics are not widely available.

Show MeSH
Related in: MedlinePlus