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Novel human bocavirus in children with acute respiratory tract infection.

Song JR, Jin Y, Xie ZP, Gao HC, Xiao NG, Chen WX, Xu ZQ, Yan KL, Zhao Y, Hou YD, Duan ZJ - Emerging Infect. Dis. (2010)

Bottom Line: Human bocavirus (HBoV) and HBoV2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection.Our results suggest that, like HBoV, HBoV2 is distributed worldwide and may be associated with respiratory and enteric diseases.

View Article: PubMed Central - PubMed

Affiliation: First Hospital of Lanzhou University, Lanzhou, People's Republic of China.

ABSTRACT
Human bocavirus (HBoV) and HBoV2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection. Our results suggest that, like HBoV, HBoV2 is distributed worldwide and may be associated with respiratory and enteric diseases.

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Related in: MedlinePlus

Phylogenetic analysis of A) the partial nonstructural protein 1 (NS1) nucleotide sequences (412 bp) and B) the partial nucleoprotein 1 (NP1) nucleotide sequences (256 bp) of human bocavirus 2 (HBoV2), Gansu Province, People’s Republic of China. The phylogenetic trees were constructed by the neighbor-joining method using MEGA 3.1 (www.megasoftware.net), and bootstrap values were determined for 1,000 replicates. Bootstrap values >50% are shown at the branching points. Human bocavirus (HBoV) and HBoV2 reference sequences are indicated by circles and squares, respectively. Bovine parvovirus (BPV), cytomegalovirus (CMV), and human parvovirus B19 (B19) reference sequences are indicated by inverted triangles. Scale bars indicate nucleotide substitutions per site. Reference sequences were obtained from GenBank (accession nos. DQ000495, DQ000496, EF450717, EU082213, EU918736, EU984232, FJ170278, FJ170279, FJ170280, NC001540, NC004442, and NC000883). Sequences generated in this study were deposited in GenBank under accession nos. FJ911558–FJ911600.
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Figure 1: Phylogenetic analysis of A) the partial nonstructural protein 1 (NS1) nucleotide sequences (412 bp) and B) the partial nucleoprotein 1 (NP1) nucleotide sequences (256 bp) of human bocavirus 2 (HBoV2), Gansu Province, People’s Republic of China. The phylogenetic trees were constructed by the neighbor-joining method using MEGA 3.1 (www.megasoftware.net), and bootstrap values were determined for 1,000 replicates. Bootstrap values >50% are shown at the branching points. Human bocavirus (HBoV) and HBoV2 reference sequences are indicated by circles and squares, respectively. Bovine parvovirus (BPV), cytomegalovirus (CMV), and human parvovirus B19 (B19) reference sequences are indicated by inverted triangles. Scale bars indicate nucleotide substitutions per site. Reference sequences were obtained from GenBank (accession nos. DQ000495, DQ000496, EF450717, EU082213, EU918736, EU984232, FJ170278, FJ170279, FJ170280, NC001540, NC004442, and NC000883). Sequences generated in this study were deposited in GenBank under accession nos. FJ911558–FJ911600.

Mentions: In total, 260 viruses were identified in 196 (83.4%) of the 235 children. Using nested PCR, we found 21 positive specimens; further nucleotide sequence analysis showed that 10 (4.3%) were HBoV2 and 11 were HBoV (Figure, panel A). All 11 HBoV strains detected by using HBoV2 nested-PCR were included in the 18 HBoV-positive patients as determined by PCR using primers 188F and 542R. Of the 10 HBoV2-positive patients, 7 (70%) were co-infected with other respiratory viruses, including 4 patients with RSV. Of the 18 HBoV-positive patients, 12 (66.7%) displayed co-infections. There were no statistically significant differences in the HBoV2 and HBoV detection (p = 0.119 by χ2 test) and co-infection (p = 1.000 by Fisher exact test) rates.


Novel human bocavirus in children with acute respiratory tract infection.

Song JR, Jin Y, Xie ZP, Gao HC, Xiao NG, Chen WX, Xu ZQ, Yan KL, Zhao Y, Hou YD, Duan ZJ - Emerging Infect. Dis. (2010)

Phylogenetic analysis of A) the partial nonstructural protein 1 (NS1) nucleotide sequences (412 bp) and B) the partial nucleoprotein 1 (NP1) nucleotide sequences (256 bp) of human bocavirus 2 (HBoV2), Gansu Province, People’s Republic of China. The phylogenetic trees were constructed by the neighbor-joining method using MEGA 3.1 (www.megasoftware.net), and bootstrap values were determined for 1,000 replicates. Bootstrap values >50% are shown at the branching points. Human bocavirus (HBoV) and HBoV2 reference sequences are indicated by circles and squares, respectively. Bovine parvovirus (BPV), cytomegalovirus (CMV), and human parvovirus B19 (B19) reference sequences are indicated by inverted triangles. Scale bars indicate nucleotide substitutions per site. Reference sequences were obtained from GenBank (accession nos. DQ000495, DQ000496, EF450717, EU082213, EU918736, EU984232, FJ170278, FJ170279, FJ170280, NC001540, NC004442, and NC000883). Sequences generated in this study were deposited in GenBank under accession nos. FJ911558–FJ911600.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2957997&req=5

Figure 1: Phylogenetic analysis of A) the partial nonstructural protein 1 (NS1) nucleotide sequences (412 bp) and B) the partial nucleoprotein 1 (NP1) nucleotide sequences (256 bp) of human bocavirus 2 (HBoV2), Gansu Province, People’s Republic of China. The phylogenetic trees were constructed by the neighbor-joining method using MEGA 3.1 (www.megasoftware.net), and bootstrap values were determined for 1,000 replicates. Bootstrap values >50% are shown at the branching points. Human bocavirus (HBoV) and HBoV2 reference sequences are indicated by circles and squares, respectively. Bovine parvovirus (BPV), cytomegalovirus (CMV), and human parvovirus B19 (B19) reference sequences are indicated by inverted triangles. Scale bars indicate nucleotide substitutions per site. Reference sequences were obtained from GenBank (accession nos. DQ000495, DQ000496, EF450717, EU082213, EU918736, EU984232, FJ170278, FJ170279, FJ170280, NC001540, NC004442, and NC000883). Sequences generated in this study were deposited in GenBank under accession nos. FJ911558–FJ911600.
Mentions: In total, 260 viruses were identified in 196 (83.4%) of the 235 children. Using nested PCR, we found 21 positive specimens; further nucleotide sequence analysis showed that 10 (4.3%) were HBoV2 and 11 were HBoV (Figure, panel A). All 11 HBoV strains detected by using HBoV2 nested-PCR were included in the 18 HBoV-positive patients as determined by PCR using primers 188F and 542R. Of the 10 HBoV2-positive patients, 7 (70%) were co-infected with other respiratory viruses, including 4 patients with RSV. Of the 18 HBoV-positive patients, 12 (66.7%) displayed co-infections. There were no statistically significant differences in the HBoV2 and HBoV detection (p = 0.119 by χ2 test) and co-infection (p = 1.000 by Fisher exact test) rates.

Bottom Line: Human bocavirus (HBoV) and HBoV2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection.Our results suggest that, like HBoV, HBoV2 is distributed worldwide and may be associated with respiratory and enteric diseases.

View Article: PubMed Central - PubMed

Affiliation: First Hospital of Lanzhou University, Lanzhou, People's Republic of China.

ABSTRACT
Human bocavirus (HBoV) and HBoV2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection. Our results suggest that, like HBoV, HBoV2 is distributed worldwide and may be associated with respiratory and enteric diseases.

Show MeSH
Related in: MedlinePlus