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Hepatitis E virus epidemiology in industrialized countries.

Clemente-Casares P, Pina S, Buti M, Jardi R, MartIn M, Bofill-Mas S, Girones R - Emerging Infect. Dis. (2003)

Bottom Line: We identified 15 HEV strains, which were similar to two HEV isolates previously described in Barcelona in clinical samples and to strains from diverse geographic HEV-nonendemic areas.We also identified two HEV strains in sewage samples from Washington, D.C., and Nancy, France; these samples were also positive for Hepatitis A virus.In addition, we studied the role of pigs as a reservoir for HEV and identified one new swine HEV strain.

View Article: PubMed Central - PubMed

Affiliation: University of Barcelona, Barcelona, Spain.

ABSTRACT
To determine the prevalence of Hepatitis E virus (HEV) in industrialized nations, we analyzed the excretion of HEV strains by the populations of Spain, France, Greece, Sweden, and the United States. Twenty of 46 (43.5%) urban sewage samples collected in Barcelona from 1994 to 2002 tested positive for HEV. We identified 15 HEV strains, which were similar to two HEV isolates previously described in Barcelona in clinical samples and to strains from diverse geographic HEV-nonendemic areas. We also identified two HEV strains in sewage samples from Washington, D.C., and Nancy, France; these samples were also positive for Hepatitis A virus. In addition, we studied the role of pigs as a reservoir for HEV and identified one new swine HEV strain. Our results suggest that HEV may be more prevalent than previously considered in industrialized countries and that variants of the virus circulate simultaneously in one region.

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Nucleotide alignment of the amplified fragment within ORF2 from some representative isolates in this study with other Hepatitis E virus strains. Dots indicate sequence identities.
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Figure 2: Nucleotide alignment of the amplified fragment within ORF2 from some representative isolates in this study with other Hepatitis E virus strains. Dots indicate sequence identities.

Mentions: Phylogenetic analysis showed that all the sequences detected were grouped with strains isolated in countries where HEV was considered nonendemic (Figure 1). The nucleotide sequence alignment of the detected strains with other HEV strains is shown in Figure 2. BCN2–9, BCN11–BCN14, and BCN16 shared a 91.0% to 99.2% similarity. They were closely related to VH1 (94.3% to 98.4% identity) and VH2 (91.0% to 95.1%), two strains isolated from clinical samples in the same area (Barcelona), previously described (15), and G1 (92.7% to 96.7%), an isolate from Greece. Two isolates (BCN10 and BCN15) showed substantially different sequences, sharing 86.5% to 89.2% and 87.2% to 91.9% similarity with the rest of strains detected in Barcelona. A previous study (15) in the same area with specific primers for HEV strains from areas where the virus is endemic identified one isolate (BCN), which was closely related to Indian strains. Nucleotide sequence identities among this isolate and the new ones from Barcelona ranged from 79.7% to 83.7%.


Hepatitis E virus epidemiology in industrialized countries.

Clemente-Casares P, Pina S, Buti M, Jardi R, MartIn M, Bofill-Mas S, Girones R - Emerging Infect. Dis. (2003)

Nucleotide alignment of the amplified fragment within ORF2 from some representative isolates in this study with other Hepatitis E virus strains. Dots indicate sequence identities.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2957966&req=5

Figure 2: Nucleotide alignment of the amplified fragment within ORF2 from some representative isolates in this study with other Hepatitis E virus strains. Dots indicate sequence identities.
Mentions: Phylogenetic analysis showed that all the sequences detected were grouped with strains isolated in countries where HEV was considered nonendemic (Figure 1). The nucleotide sequence alignment of the detected strains with other HEV strains is shown in Figure 2. BCN2–9, BCN11–BCN14, and BCN16 shared a 91.0% to 99.2% similarity. They were closely related to VH1 (94.3% to 98.4% identity) and VH2 (91.0% to 95.1%), two strains isolated from clinical samples in the same area (Barcelona), previously described (15), and G1 (92.7% to 96.7%), an isolate from Greece. Two isolates (BCN10 and BCN15) showed substantially different sequences, sharing 86.5% to 89.2% and 87.2% to 91.9% similarity with the rest of strains detected in Barcelona. A previous study (15) in the same area with specific primers for HEV strains from areas where the virus is endemic identified one isolate (BCN), which was closely related to Indian strains. Nucleotide sequence identities among this isolate and the new ones from Barcelona ranged from 79.7% to 83.7%.

Bottom Line: We identified 15 HEV strains, which were similar to two HEV isolates previously described in Barcelona in clinical samples and to strains from diverse geographic HEV-nonendemic areas.We also identified two HEV strains in sewage samples from Washington, D.C., and Nancy, France; these samples were also positive for Hepatitis A virus.In addition, we studied the role of pigs as a reservoir for HEV and identified one new swine HEV strain.

View Article: PubMed Central - PubMed

Affiliation: University of Barcelona, Barcelona, Spain.

ABSTRACT
To determine the prevalence of Hepatitis E virus (HEV) in industrialized nations, we analyzed the excretion of HEV strains by the populations of Spain, France, Greece, Sweden, and the United States. Twenty of 46 (43.5%) urban sewage samples collected in Barcelona from 1994 to 2002 tested positive for HEV. We identified 15 HEV strains, which were similar to two HEV isolates previously described in Barcelona in clinical samples and to strains from diverse geographic HEV-nonendemic areas. We also identified two HEV strains in sewage samples from Washington, D.C., and Nancy, France; these samples were also positive for Hepatitis A virus. In addition, we studied the role of pigs as a reservoir for HEV and identified one new swine HEV strain. Our results suggest that HEV may be more prevalent than previously considered in industrialized countries and that variants of the virus circulate simultaneously in one region.

Show MeSH
Related in: MedlinePlus