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Nanoparticles induce changes of the electrical activity of neuronal networks on microelectrode array neurochips.

Gramowski A, Flossdorf J, Bhattacharya K, Jonas L, Lantow M, Rahman Q, Schiffmann D, Weiss DG, Dopp E - Environ. Health Perspect. (2010)

Bottom Line: The number of action potentials and the frequency of their patterns (spike and burst rates) showed a significant particle-dependent decrease and significant differences in potency.Additionally, 24 hr exposure to TiO2 NPs caused intracellular formation of ROS in neuronal and glial cells, whereas exposure to CB and Fe2O3 NPs up to a concentration of 10 µg/cm2 did not induce significant changes in free radical levels.NPs at low particle concentrations are able to exhibit a neurotoxic effect by disturbing the electrical activity of neuronal networks, but the underlying mechanisms depend on the particle type.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Cell Biology and Biosystems Technology, University of Rostock, Rostock, Germany.

ABSTRACT

Background: Nanomaterials are extensively used in industry and daily life, but little is known about possible health effects. An intensified research regarding toxicity of nanomaterials is urgently needed. Several studies have demonstrated that nanoparticles (NPs; diameter < 100 nm) can be transported to the central nervous system; however, interference of NPs with the electrical activity of neurons has not yet been shown.

Objectives/methods: We investigated the acute electrophysiological effects of carbon black (CB), hematite (Fe2O3), and titanium dioxide (TiO2) NPs in primary murine cortical networks on microelectrode array (MEA) neurochips. Uptake of NPs was studied by transmission electron microscopy (TEM), and intracellular formation of reactive oxygen species (ROS) was studied by flow cytometry.

Results: The multiparametric assessment of electrical activity changes caused by the NPs revealed an NP-specific and concentration-dependent inhibition of the firing patterns. The number of action potentials and the frequency of their patterns (spike and burst rates) showed a significant particle-dependent decrease and significant differences in potency. Further, we detected the uptake of CB, Fe2O3, and TiO2 into glial cells and neurons by TEM. Additionally, 24 hr exposure to TiO2 NPs caused intracellular formation of ROS in neuronal and glial cells, whereas exposure to CB and Fe2O3 NPs up to a concentration of 10 µg/cm2 did not induce significant changes in free radical levels.

Conclusion: NPs at low particle concentrations are able to exhibit a neurotoxic effect by disturbing the electrical activity of neuronal networks, but the underlying mechanisms depend on the particle type.

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Related in: MedlinePlus

REM (A) and TEM (B) images of neuronal networks exposed to TiO2-NPs. (A) Neuronal cell with internalized TiO2 NPs (arrows). (B) Accumulation of particles inside lysosomes (large arrow) and residual bodies (small arrow). In A, bar = 5 μm; in B, bar = 1 μm.
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f7-ehp-118-1363: REM (A) and TEM (B) images of neuronal networks exposed to TiO2-NPs. (A) Neuronal cell with internalized TiO2 NPs (arrows). (B) Accumulation of particles inside lysosomes (large arrow) and residual bodies (small arrow). In A, bar = 5 μm; in B, bar = 1 μm.

Mentions: Figure 7 demonstrates the uptake of TiO2 into neuronal network cells as well as an accumulation of particles or particle agglomerates in a space near the cell surface.


Nanoparticles induce changes of the electrical activity of neuronal networks on microelectrode array neurochips.

Gramowski A, Flossdorf J, Bhattacharya K, Jonas L, Lantow M, Rahman Q, Schiffmann D, Weiss DG, Dopp E - Environ. Health Perspect. (2010)

REM (A) and TEM (B) images of neuronal networks exposed to TiO2-NPs. (A) Neuronal cell with internalized TiO2 NPs (arrows). (B) Accumulation of particles inside lysosomes (large arrow) and residual bodies (small arrow). In A, bar = 5 μm; in B, bar = 1 μm.
© Copyright Policy - public-domain
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2957913&req=5

f7-ehp-118-1363: REM (A) and TEM (B) images of neuronal networks exposed to TiO2-NPs. (A) Neuronal cell with internalized TiO2 NPs (arrows). (B) Accumulation of particles inside lysosomes (large arrow) and residual bodies (small arrow). In A, bar = 5 μm; in B, bar = 1 μm.
Mentions: Figure 7 demonstrates the uptake of TiO2 into neuronal network cells as well as an accumulation of particles or particle agglomerates in a space near the cell surface.

Bottom Line: The number of action potentials and the frequency of their patterns (spike and burst rates) showed a significant particle-dependent decrease and significant differences in potency.Additionally, 24 hr exposure to TiO2 NPs caused intracellular formation of ROS in neuronal and glial cells, whereas exposure to CB and Fe2O3 NPs up to a concentration of 10 µg/cm2 did not induce significant changes in free radical levels.NPs at low particle concentrations are able to exhibit a neurotoxic effect by disturbing the electrical activity of neuronal networks, but the underlying mechanisms depend on the particle type.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Cell Biology and Biosystems Technology, University of Rostock, Rostock, Germany.

ABSTRACT

Background: Nanomaterials are extensively used in industry and daily life, but little is known about possible health effects. An intensified research regarding toxicity of nanomaterials is urgently needed. Several studies have demonstrated that nanoparticles (NPs; diameter < 100 nm) can be transported to the central nervous system; however, interference of NPs with the electrical activity of neurons has not yet been shown.

Objectives/methods: We investigated the acute electrophysiological effects of carbon black (CB), hematite (Fe2O3), and titanium dioxide (TiO2) NPs in primary murine cortical networks on microelectrode array (MEA) neurochips. Uptake of NPs was studied by transmission electron microscopy (TEM), and intracellular formation of reactive oxygen species (ROS) was studied by flow cytometry.

Results: The multiparametric assessment of electrical activity changes caused by the NPs revealed an NP-specific and concentration-dependent inhibition of the firing patterns. The number of action potentials and the frequency of their patterns (spike and burst rates) showed a significant particle-dependent decrease and significant differences in potency. Further, we detected the uptake of CB, Fe2O3, and TiO2 into glial cells and neurons by TEM. Additionally, 24 hr exposure to TiO2 NPs caused intracellular formation of ROS in neuronal and glial cells, whereas exposure to CB and Fe2O3 NPs up to a concentration of 10 µg/cm2 did not induce significant changes in free radical levels.

Conclusion: NPs at low particle concentrations are able to exhibit a neurotoxic effect by disturbing the electrical activity of neuronal networks, but the underlying mechanisms depend on the particle type.

Show MeSH
Related in: MedlinePlus