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Sexual dimorphism in the fly brain.

Cachero S, Ostrovsky AD, Yu JY, Dickson BJ, Jefferis GS - Curr. Biol. (2010)

Bottom Line: Initial work found limited evidence for anatomical dimorphism in these fru+ neurons.Our analysis reveals substantial differences in wiring and gross anatomy between male and female fly brains.Reciprocal connection differences in the lateral horn offer a plausible explanation for opposing responses to sex pheromones in male and female flies.

View Article: PubMed Central - PubMed

Affiliation: Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, UK.

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fru+ Lateral Horn Neurons(A) Cluster analysis of lateral horn innervation patterns of many examples of five classes of fru+ lateral horn neuron (LHN). Color bars indicate class and sex, with five hues (blue, red, orange, magenta, and green) representing male clones and matched but paler colors representing female clones. For four of these classes, clones of the same sex cocluster but clones of the same class from different sexes do not. The exception is aIP-b, in which male and female samples do not separate (light and dark blue). See Supplemental Experimental Procedures for details.(B) Volume rendering of seven classes of fru+ LHNs in males and females. Neuronal arbors are colored based on UAS:synaptotagmin staining and neuronal morphology. Cell bodies are in red, axons are in green, dendrites are in blue, unclassified neurites are in black. We did not obtain UAS:synaptotagmin data for clone aSP-a or the male aSP-h clone.(C) The bundle of aDT-a axons (red) traversing the LH serves as a simple landmark to separate pheromone and general odor zones of the lateral horn (dotted white line) [7].(D1 and D2) Processes of all male (D1) and female (D2) fru+ LHNs (magenta) are restricted to the pheromone zone. Only LHN neurites within the LH are shown. Scale bar represents 50 μm. See also Figure S3.
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fig5: fru+ Lateral Horn Neurons(A) Cluster analysis of lateral horn innervation patterns of many examples of five classes of fru+ lateral horn neuron (LHN). Color bars indicate class and sex, with five hues (blue, red, orange, magenta, and green) representing male clones and matched but paler colors representing female clones. For four of these classes, clones of the same sex cocluster but clones of the same class from different sexes do not. The exception is aIP-b, in which male and female samples do not separate (light and dark blue). See Supplemental Experimental Procedures for details.(B) Volume rendering of seven classes of fru+ LHNs in males and females. Neuronal arbors are colored based on UAS:synaptotagmin staining and neuronal morphology. Cell bodies are in red, axons are in green, dendrites are in blue, unclassified neurites are in black. We did not obtain UAS:synaptotagmin data for clone aSP-a or the male aSP-h clone.(C) The bundle of aDT-a axons (red) traversing the LH serves as a simple landmark to separate pheromone and general odor zones of the lateral horn (dotted white line) [7].(D1 and D2) Processes of all male (D1) and female (D2) fru+ LHNs (magenta) are restricted to the pheromone zone. Only LHN neurites within the LH are shown. Scale bar represents 50 μm. See also Figure S3.

Mentions: Most PN axons make en passant connections in the mushroom body calyx before terminating in the lateral horn (LH). It has previously been shown that many mushroom body neurons are fru+. We confirmed this but found no overt anatomical dimorphism in these cells. In the LH, we identified eight fru+ third-order olfactory neuron lineages. In contrast to the mushroom bodies, seven of eight LH clones have dimorphic projections (Figure 2; Figure 5); three also differ in cell number (Table 1). The sole exception is a pair of local interneurons (pSP-f; see Figure S2). The seven dimorphic clones all have input regions in the LH and output regions in other parts of the brain and can therefore be considered to be principal neurons of the lateral horn (LHNs).


Sexual dimorphism in the fly brain.

Cachero S, Ostrovsky AD, Yu JY, Dickson BJ, Jefferis GS - Curr. Biol. (2010)

fru+ Lateral Horn Neurons(A) Cluster analysis of lateral horn innervation patterns of many examples of five classes of fru+ lateral horn neuron (LHN). Color bars indicate class and sex, with five hues (blue, red, orange, magenta, and green) representing male clones and matched but paler colors representing female clones. For four of these classes, clones of the same sex cocluster but clones of the same class from different sexes do not. The exception is aIP-b, in which male and female samples do not separate (light and dark blue). See Supplemental Experimental Procedures for details.(B) Volume rendering of seven classes of fru+ LHNs in males and females. Neuronal arbors are colored based on UAS:synaptotagmin staining and neuronal morphology. Cell bodies are in red, axons are in green, dendrites are in blue, unclassified neurites are in black. We did not obtain UAS:synaptotagmin data for clone aSP-a or the male aSP-h clone.(C) The bundle of aDT-a axons (red) traversing the LH serves as a simple landmark to separate pheromone and general odor zones of the lateral horn (dotted white line) [7].(D1 and D2) Processes of all male (D1) and female (D2) fru+ LHNs (magenta) are restricted to the pheromone zone. Only LHN neurites within the LH are shown. Scale bar represents 50 μm. See also Figure S3.
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fig5: fru+ Lateral Horn Neurons(A) Cluster analysis of lateral horn innervation patterns of many examples of five classes of fru+ lateral horn neuron (LHN). Color bars indicate class and sex, with five hues (blue, red, orange, magenta, and green) representing male clones and matched but paler colors representing female clones. For four of these classes, clones of the same sex cocluster but clones of the same class from different sexes do not. The exception is aIP-b, in which male and female samples do not separate (light and dark blue). See Supplemental Experimental Procedures for details.(B) Volume rendering of seven classes of fru+ LHNs in males and females. Neuronal arbors are colored based on UAS:synaptotagmin staining and neuronal morphology. Cell bodies are in red, axons are in green, dendrites are in blue, unclassified neurites are in black. We did not obtain UAS:synaptotagmin data for clone aSP-a or the male aSP-h clone.(C) The bundle of aDT-a axons (red) traversing the LH serves as a simple landmark to separate pheromone and general odor zones of the lateral horn (dotted white line) [7].(D1 and D2) Processes of all male (D1) and female (D2) fru+ LHNs (magenta) are restricted to the pheromone zone. Only LHN neurites within the LH are shown. Scale bar represents 50 μm. See also Figure S3.
Mentions: Most PN axons make en passant connections in the mushroom body calyx before terminating in the lateral horn (LH). It has previously been shown that many mushroom body neurons are fru+. We confirmed this but found no overt anatomical dimorphism in these cells. In the LH, we identified eight fru+ third-order olfactory neuron lineages. In contrast to the mushroom bodies, seven of eight LH clones have dimorphic projections (Figure 2; Figure 5); three also differ in cell number (Table 1). The sole exception is a pair of local interneurons (pSP-f; see Figure S2). The seven dimorphic clones all have input regions in the LH and output regions in other parts of the brain and can therefore be considered to be principal neurons of the lateral horn (LHNs).

Bottom Line: Initial work found limited evidence for anatomical dimorphism in these fru+ neurons.Our analysis reveals substantial differences in wiring and gross anatomy between male and female fly brains.Reciprocal connection differences in the lateral horn offer a plausible explanation for opposing responses to sex pheromones in male and female flies.

View Article: PubMed Central - PubMed

Affiliation: Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, UK.

Show MeSH
Related in: MedlinePlus