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A Strong Impact of Genetic Background on Gut Microflora in Mice.

Esworthy RS, Smith DD, Chu FF - Int J Inflam (2010)

Bottom Line: We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets.We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes.From 129 DKO strictly, we found overgrowth of Escherichia coli.

View Article: PubMed Central - PubMed

Affiliation: Department of Cancer Biology, Beckman Research Institute of the City of Hope, 1500 Duarte Road, Duarte, CA 91010-3000, USA.

ABSTRACT
Genetic background affects susceptibility to ileocolitis in mice deficient in two intracellular glutathione peroxidases, GPx1 and GPx2. The C57BL/6 (B6) GPx1/2 double-knockout (DKO) mice have mild ileocolitis, and 129S1/Sv (129) DKO mice have severe inflammation. We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets. Because luminal microbiota induce DKO ileocolitis, we assessed bacterial composition with automated ribosomal intergenic-spacer analysis (ARISA) on cecal DNA. We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes. In comparing AIN and LabDiet, DKO mice were more resistant to change than the non-DKO or WT mice. However, supplementing yeast and inulin to AIN diet greatly altered microflora profiles in the DKO mice. From 129 DKO strictly, we found overgrowth of Escherichia coli. We conclude that genetic background predisposes mice to colonization of potentially pathogenic E. coli.

No MeSH data available.


Related in: MedlinePlus

Heat map of Czekanowski similarity indexes of the ARISA profiles from Figures 4, 6, and 8. An index of 0.6 (white) is moderate similarity and an index of 0 (dark orange) is most distinct. “Cont” means the non-DKO sibs control of 129 DKO mice.
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fig5: Heat map of Czekanowski similarity indexes of the ARISA profiles from Figures 4, 6, and 8. An index of 0.6 (white) is moderate similarity and an index of 0 (dark orange) is most distinct. “Cont” means the non-DKO sibs control of 129 DKO mice.

Mentions: The microbiota profiles on B6 DKO and WT mice were determined individually on either LabDiet or AIN diet, and a combined profile was generated for each group of six mice by the Ribosort package programs (Figure 4). Each electropherogram was checked against the combined panel to confirm that the dominant DNA ribotypes in the panel were a consensus feature of the group. We used Czekanowski similarity index to compare the ribotype profiles between different mouse groups (Figure 5). This index is based on both presence and abundance of ribotypes without the bias of focusing on dominant ribotypes. Similar profiles were found in B6 DKO mice on either AIN or LabDiet and B6 WT mice on the AIN diet. B6 WT on LabDiet had the most distinct ribotype profile with dramatically diminished PCR products of 385–390 bp, which were prominent in the other 3 groups. The 385–390 bp products were sequenced and identified as either Lactobacillus species or Bacteroides sp. (Table 2). This result supports the notion that DKO mice have diminished diversity and thus more resistant to diet-associated alteration of microbiota.


A Strong Impact of Genetic Background on Gut Microflora in Mice.

Esworthy RS, Smith DD, Chu FF - Int J Inflam (2010)

Heat map of Czekanowski similarity indexes of the ARISA profiles from Figures 4, 6, and 8. An index of 0.6 (white) is moderate similarity and an index of 0 (dark orange) is most distinct. “Cont” means the non-DKO sibs control of 129 DKO mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2957666&req=5

fig5: Heat map of Czekanowski similarity indexes of the ARISA profiles from Figures 4, 6, and 8. An index of 0.6 (white) is moderate similarity and an index of 0 (dark orange) is most distinct. “Cont” means the non-DKO sibs control of 129 DKO mice.
Mentions: The microbiota profiles on B6 DKO and WT mice were determined individually on either LabDiet or AIN diet, and a combined profile was generated for each group of six mice by the Ribosort package programs (Figure 4). Each electropherogram was checked against the combined panel to confirm that the dominant DNA ribotypes in the panel were a consensus feature of the group. We used Czekanowski similarity index to compare the ribotype profiles between different mouse groups (Figure 5). This index is based on both presence and abundance of ribotypes without the bias of focusing on dominant ribotypes. Similar profiles were found in B6 DKO mice on either AIN or LabDiet and B6 WT mice on the AIN diet. B6 WT on LabDiet had the most distinct ribotype profile with dramatically diminished PCR products of 385–390 bp, which were prominent in the other 3 groups. The 385–390 bp products were sequenced and identified as either Lactobacillus species or Bacteroides sp. (Table 2). This result supports the notion that DKO mice have diminished diversity and thus more resistant to diet-associated alteration of microbiota.

Bottom Line: We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets.We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes.From 129 DKO strictly, we found overgrowth of Escherichia coli.

View Article: PubMed Central - PubMed

Affiliation: Department of Cancer Biology, Beckman Research Institute of the City of Hope, 1500 Duarte Road, Duarte, CA 91010-3000, USA.

ABSTRACT
Genetic background affects susceptibility to ileocolitis in mice deficient in two intracellular glutathione peroxidases, GPx1 and GPx2. The C57BL/6 (B6) GPx1/2 double-knockout (DKO) mice have mild ileocolitis, and 129S1/Sv (129) DKO mice have severe inflammation. We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets. Because luminal microbiota induce DKO ileocolitis, we assessed bacterial composition with automated ribosomal intergenic-spacer analysis (ARISA) on cecal DNA. We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes. In comparing AIN and LabDiet, DKO mice were more resistant to change than the non-DKO or WT mice. However, supplementing yeast and inulin to AIN diet greatly altered microflora profiles in the DKO mice. From 129 DKO strictly, we found overgrowth of Escherichia coli. We conclude that genetic background predisposes mice to colonization of potentially pathogenic E. coli.

No MeSH data available.


Related in: MedlinePlus