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A Strong Impact of Genetic Background on Gut Microflora in Mice.

Esworthy RS, Smith DD, Chu FF - Int J Inflam (2010)

Bottom Line: We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets.We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes.From 129 DKO strictly, we found overgrowth of Escherichia coli.

View Article: PubMed Central - PubMed

Affiliation: Department of Cancer Biology, Beckman Research Institute of the City of Hope, 1500 Duarte Road, Duarte, CA 91010-3000, USA.

ABSTRACT
Genetic background affects susceptibility to ileocolitis in mice deficient in two intracellular glutathione peroxidases, GPx1 and GPx2. The C57BL/6 (B6) GPx1/2 double-knockout (DKO) mice have mild ileocolitis, and 129S1/Sv (129) DKO mice have severe inflammation. We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets. Because luminal microbiota induce DKO ileocolitis, we assessed bacterial composition with automated ribosomal intergenic-spacer analysis (ARISA) on cecal DNA. We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes. In comparing AIN and LabDiet, DKO mice were more resistant to change than the non-DKO or WT mice. However, supplementing yeast and inulin to AIN diet greatly altered microflora profiles in the DKO mice. From 129 DKO strictly, we found overgrowth of Escherichia coli. We conclude that genetic background predisposes mice to colonization of potentially pathogenic E. coli.

No MeSH data available.


Related in: MedlinePlus

Kaplan-Meier curves showing the effect of genetic background and diet on DKO mouse survival. All mice are on AIN base diets. Monitored from 8 days of age, the surviving fraction excludes dead and culled, moribund mice. The numbers in parenthesis are the number of mice in each group. The data for yeast are pools both 1 and 10% as no difference could be distinguished. There are log rank differences for all interstrain comparisons on AIN diet: P < .0001. 129 N5, yeast-supplemented AIN (AIN + yeast) diet versus AIN; P = .0087. 129 N10, inulin-supplemented AIN (Ain + inulin) diet versus AIN; P = .0003.
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fig1: Kaplan-Meier curves showing the effect of genetic background and diet on DKO mouse survival. All mice are on AIN base diets. Monitored from 8 days of age, the surviving fraction excludes dead and culled, moribund mice. The numbers in parenthesis are the number of mice in each group. The data for yeast are pools both 1 and 10% as no difference could be distinguished. There are log rank differences for all interstrain comparisons on AIN diet: P < .0001. 129 N5, yeast-supplemented AIN (AIN + yeast) diet versus AIN; P = .0087. 129 N10, inulin-supplemented AIN (Ain + inulin) diet versus AIN; P = .0003.

Mentions: Mouse strain background has a big effect on morbidity outcome in GPx1/2-DKO mice (Figure 1). As we noted before the strain difference in survival on the conventional LabDiet, here we report the same genetic effect on mouse survival on the AIN diet. The AIN diet, formulated to mimic LabDiet for calories, macro- and micronutrients, maintained better health than LabDiets for the DKO mice.


A Strong Impact of Genetic Background on Gut Microflora in Mice.

Esworthy RS, Smith DD, Chu FF - Int J Inflam (2010)

Kaplan-Meier curves showing the effect of genetic background and diet on DKO mouse survival. All mice are on AIN base diets. Monitored from 8 days of age, the surviving fraction excludes dead and culled, moribund mice. The numbers in parenthesis are the number of mice in each group. The data for yeast are pools both 1 and 10% as no difference could be distinguished. There are log rank differences for all interstrain comparisons on AIN diet: P < .0001. 129 N5, yeast-supplemented AIN (AIN + yeast) diet versus AIN; P = .0087. 129 N10, inulin-supplemented AIN (Ain + inulin) diet versus AIN; P = .0003.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2957666&req=5

fig1: Kaplan-Meier curves showing the effect of genetic background and diet on DKO mouse survival. All mice are on AIN base diets. Monitored from 8 days of age, the surviving fraction excludes dead and culled, moribund mice. The numbers in parenthesis are the number of mice in each group. The data for yeast are pools both 1 and 10% as no difference could be distinguished. There are log rank differences for all interstrain comparisons on AIN diet: P < .0001. 129 N5, yeast-supplemented AIN (AIN + yeast) diet versus AIN; P = .0087. 129 N10, inulin-supplemented AIN (Ain + inulin) diet versus AIN; P = .0003.
Mentions: Mouse strain background has a big effect on morbidity outcome in GPx1/2-DKO mice (Figure 1). As we noted before the strain difference in survival on the conventional LabDiet, here we report the same genetic effect on mouse survival on the AIN diet. The AIN diet, formulated to mimic LabDiet for calories, macro- and micronutrients, maintained better health than LabDiets for the DKO mice.

Bottom Line: We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets.We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes.From 129 DKO strictly, we found overgrowth of Escherichia coli.

View Article: PubMed Central - PubMed

Affiliation: Department of Cancer Biology, Beckman Research Institute of the City of Hope, 1500 Duarte Road, Duarte, CA 91010-3000, USA.

ABSTRACT
Genetic background affects susceptibility to ileocolitis in mice deficient in two intracellular glutathione peroxidases, GPx1 and GPx2. The C57BL/6 (B6) GPx1/2 double-knockout (DKO) mice have mild ileocolitis, and 129S1/Sv (129) DKO mice have severe inflammation. We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets. Because luminal microbiota induce DKO ileocolitis, we assessed bacterial composition with automated ribosomal intergenic-spacer analysis (ARISA) on cecal DNA. We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes. In comparing AIN and LabDiet, DKO mice were more resistant to change than the non-DKO or WT mice. However, supplementing yeast and inulin to AIN diet greatly altered microflora profiles in the DKO mice. From 129 DKO strictly, we found overgrowth of Escherichia coli. We conclude that genetic background predisposes mice to colonization of potentially pathogenic E. coli.

No MeSH data available.


Related in: MedlinePlus