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Exercise training improves age-related myocardial metabolic derangement: proton magnetic resonance spectroscopy study in the rat model.

Choi SI, Chang HJ, Chun EJ, Cho SB, Kim ST, Yoon YE, Chang SA, Kim JH, Kim CH, Lim TH - Korean Circ J (2010)

Bottom Line: The creatine-to-water ratios in the interventricular septa of YC did not differ significantly from that of OT (0.00131±0.00025 vs. 0.00127±0.00031; p=0.37).However, OC showed significant reduction in creatine-to-water ratio compared to OT (0.00096±0.00025 vs. 0.00127±0.00031; p<0.001).In contrast, the mean total creatine concentration of OC was significantly reduced compared to OT (6.8±3.2 vs. 11.5±4.1 mmol/kg wet weight; p=0.03).

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Seoul National University Bundang Hospital, Seongnam, Korea.

ABSTRACT

Background and objectives: The objective of this study was to determine whether long-term exercise training will improve age-related cardiac metabolic derangement using proton magnetic resonance (MR) spectroscopy.

Materials and methods: Young and old male Fischer 344 rats were assigned to sedentary controls groups {young control (YC) group-3 months of age: YC, n=10; old control (OC) group-22 months of age: OC, n=10}, and an exercise training group (OT, n=5). After 12-week of treadmill exercise training, MR spectroscopy at 4.7 T was performed to assess myocardial energy metabolism: measurements of myocardial creatine-to-water ratio (Scr/Sw) were performed using the XWIN-NMR software.

Results: Exercise capacity was 14.7 minutes greater in OT than that in OC (20.1±1.9 minutes in OT, 5.4±2.3 minutes in OC; p<0.001). The 12-week exercise training rendered the old rats a maximum exercise capacity matching that of untrained YC rats (17.9±1.5 minutes in YC, 20.1±1.9 minutes in OT; p>0.05). The creatine-to-water ratios in the interventricular septa of YC did not differ significantly from that of OT (0.00131±0.00025 vs. 0.00127±0.00031; p=0.37). However, OC showed significant reduction in creatine-to-water ratio compared to OT (0.00096±0.00025 vs. 0.00127±0.00031; p<0.001). Mean total creatine concentrations in the myocardium were similar between YC and OT (13.3±3.6 vs. 11.5±4.1 mmol/kg wet weight; p=0.29). In contrast, the mean total creatine concentration of OC was significantly reduced compared to OT (6.8±3.2 vs. 11.5±4.1 mmol/kg wet weight; p=0.03).

Conclusion: Our findings suggest that long-term exercise training in old rats induced prevention of age-related deterioration in myocardial metabolism.

No MeSH data available.


1H-spectra using a 2×2×3 mm voxel (white box) positioned in the septum (A) were obtained from a heart of the young control group (B, left), the old control group (B, middle), and the exercise training group (B, right). The spectrum of young control group and the exercise training group show no significant difference in myocardial creatine-to-water ratio. However, decreased creatine-to-water ratio was detectable in the old control group.
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Figure 1: 1H-spectra using a 2×2×3 mm voxel (white box) positioned in the septum (A) were obtained from a heart of the young control group (B, left), the old control group (B, middle), and the exercise training group (B, right). The spectrum of young control group and the exercise training group show no significant difference in myocardial creatine-to-water ratio. However, decreased creatine-to-water ratio was detectable in the old control group.

Mentions: The creatine-to-water ratios in the interventericular septum of YC did not differ significantly from values obtained in OT (0.00131±0.00025 vs. 0.00127±0.00031; p=0.37). However, OC showed significant reductions in creatine-to-water ratio compared to OT (0.00096±0.00025 vs. 0.00127±0.00031; p<0.0). Therefore, OC is characterized by substantially reduced myocardial creatine levels, whereas OT maintained equivalent levels found in YC (Fig. 1).


Exercise training improves age-related myocardial metabolic derangement: proton magnetic resonance spectroscopy study in the rat model.

Choi SI, Chang HJ, Chun EJ, Cho SB, Kim ST, Yoon YE, Chang SA, Kim JH, Kim CH, Lim TH - Korean Circ J (2010)

1H-spectra using a 2×2×3 mm voxel (white box) positioned in the septum (A) were obtained from a heart of the young control group (B, left), the old control group (B, middle), and the exercise training group (B, right). The spectrum of young control group and the exercise training group show no significant difference in myocardial creatine-to-water ratio. However, decreased creatine-to-water ratio was detectable in the old control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2957643&req=5

Figure 1: 1H-spectra using a 2×2×3 mm voxel (white box) positioned in the septum (A) were obtained from a heart of the young control group (B, left), the old control group (B, middle), and the exercise training group (B, right). The spectrum of young control group and the exercise training group show no significant difference in myocardial creatine-to-water ratio. However, decreased creatine-to-water ratio was detectable in the old control group.
Mentions: The creatine-to-water ratios in the interventericular septum of YC did not differ significantly from values obtained in OT (0.00131±0.00025 vs. 0.00127±0.00031; p=0.37). However, OC showed significant reductions in creatine-to-water ratio compared to OT (0.00096±0.00025 vs. 0.00127±0.00031; p<0.0). Therefore, OC is characterized by substantially reduced myocardial creatine levels, whereas OT maintained equivalent levels found in YC (Fig. 1).

Bottom Line: The creatine-to-water ratios in the interventricular septa of YC did not differ significantly from that of OT (0.00131±0.00025 vs. 0.00127±0.00031; p=0.37).However, OC showed significant reduction in creatine-to-water ratio compared to OT (0.00096±0.00025 vs. 0.00127±0.00031; p<0.001).In contrast, the mean total creatine concentration of OC was significantly reduced compared to OT (6.8±3.2 vs. 11.5±4.1 mmol/kg wet weight; p=0.03).

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Seoul National University Bundang Hospital, Seongnam, Korea.

ABSTRACT

Background and objectives: The objective of this study was to determine whether long-term exercise training will improve age-related cardiac metabolic derangement using proton magnetic resonance (MR) spectroscopy.

Materials and methods: Young and old male Fischer 344 rats were assigned to sedentary controls groups {young control (YC) group-3 months of age: YC, n=10; old control (OC) group-22 months of age: OC, n=10}, and an exercise training group (OT, n=5). After 12-week of treadmill exercise training, MR spectroscopy at 4.7 T was performed to assess myocardial energy metabolism: measurements of myocardial creatine-to-water ratio (Scr/Sw) were performed using the XWIN-NMR software.

Results: Exercise capacity was 14.7 minutes greater in OT than that in OC (20.1±1.9 minutes in OT, 5.4±2.3 minutes in OC; p<0.001). The 12-week exercise training rendered the old rats a maximum exercise capacity matching that of untrained YC rats (17.9±1.5 minutes in YC, 20.1±1.9 minutes in OT; p>0.05). The creatine-to-water ratios in the interventricular septa of YC did not differ significantly from that of OT (0.00131±0.00025 vs. 0.00127±0.00031; p=0.37). However, OC showed significant reduction in creatine-to-water ratio compared to OT (0.00096±0.00025 vs. 0.00127±0.00031; p<0.001). Mean total creatine concentrations in the myocardium were similar between YC and OT (13.3±3.6 vs. 11.5±4.1 mmol/kg wet weight; p=0.29). In contrast, the mean total creatine concentration of OC was significantly reduced compared to OT (6.8±3.2 vs. 11.5±4.1 mmol/kg wet weight; p=0.03).

Conclusion: Our findings suggest that long-term exercise training in old rats induced prevention of age-related deterioration in myocardial metabolism.

No MeSH data available.