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Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus.

Lopes LC, Rollin-Pinheiro R, GuimarĂ£es AJ, Bittencourt VC, Martinez LR, Koba W, Farias SE, Nosanchuk JD, Barreto-Bergter E - PLoS Negl Trop Dis (2010)

Bottom Line: In the present study, three monoclonal antibodies (mAbs, IgG1) to S. apiospermum derived PRM were generated and their effects on S. apiospermum were examined in vitro and in vivo.In culture, addition of the PRM mAbs increased S. apiospermum conidia germination and reduced conidial phagocytosis by J774.16 macrophages.Further insights into the effects of these glycopeptides on the pathobiology of S. apiospermum may lead to new avenues for preventing and treating scedosporiosis.

View Article: PubMed Central - PubMed

Affiliation: Departments of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, United States of America.

ABSTRACT
Scedosporium apiospermum is part of the Pseudallescheria-Scedosporium complex. Peptidorhamnomannans (PRMs) are cell wall glycopeptides present in some fungi, and their structures have been characterized in S. apiospermum, S. prolificans and Sporothrix schenckii. Prior work shows that PRMs can interact with host cells and that the glycopeptides are antigenic. In the present study, three monoclonal antibodies (mAbs, IgG1) to S. apiospermum derived PRM were generated and their effects on S. apiospermum were examined in vitro and in vivo. The mAbs recognized a carbohydrate epitope on PRM. In culture, addition of the PRM mAbs increased S. apiospermum conidia germination and reduced conidial phagocytosis by J774.16 macrophages. In a murine infection model, mice treated with antibodies to PRM died prior to control animals. Thus, PRM is involved in morphogenesis and the binding of this glycopeptide by mAbs enhanced the virulence of the fungus. Further insights into the effects of these glycopeptides on the pathobiology of S. apiospermum may lead to new avenues for preventing and treating scedosporiosis.

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Impact of mAbs to PRM on the release of reactive oxygen species.Opsonization of S. apiospermum conidia with mAbs to PRM suppressed the release of superoxide by J774.16 cells (* P<0.05) (A). The production of nitric oxide by J774.16 cells was not affected by the presence or absence of mAb (B). The experiments were performed three times.
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pntd-0000853-g006: Impact of mAbs to PRM on the release of reactive oxygen species.Opsonization of S. apiospermum conidia with mAbs to PRM suppressed the release of superoxide by J774.16 cells (* P<0.05) (A). The production of nitric oxide by J774.16 cells was not affected by the presence or absence of mAb (B). The experiments were performed three times.

Mentions: Addition of mAbs F10, C7 and C11 altered superoxide production by macrophages. A decrease in superoxide production occurred in the presence of the mAbs in comparison with the controls (p<0.05) (Figure 6A). In contrast, the release of nitric oxide by J774.16 cells co-cultured with S. apiospermum conidia was not affected by opsonization (Figure 6B).


Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus.

Lopes LC, Rollin-Pinheiro R, GuimarĂ£es AJ, Bittencourt VC, Martinez LR, Koba W, Farias SE, Nosanchuk JD, Barreto-Bergter E - PLoS Negl Trop Dis (2010)

Impact of mAbs to PRM on the release of reactive oxygen species.Opsonization of S. apiospermum conidia with mAbs to PRM suppressed the release of superoxide by J774.16 cells (* P<0.05) (A). The production of nitric oxide by J774.16 cells was not affected by the presence or absence of mAb (B). The experiments were performed three times.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2957425&req=5

pntd-0000853-g006: Impact of mAbs to PRM on the release of reactive oxygen species.Opsonization of S. apiospermum conidia with mAbs to PRM suppressed the release of superoxide by J774.16 cells (* P<0.05) (A). The production of nitric oxide by J774.16 cells was not affected by the presence or absence of mAb (B). The experiments were performed three times.
Mentions: Addition of mAbs F10, C7 and C11 altered superoxide production by macrophages. A decrease in superoxide production occurred in the presence of the mAbs in comparison with the controls (p<0.05) (Figure 6A). In contrast, the release of nitric oxide by J774.16 cells co-cultured with S. apiospermum conidia was not affected by opsonization (Figure 6B).

Bottom Line: In the present study, three monoclonal antibodies (mAbs, IgG1) to S. apiospermum derived PRM were generated and their effects on S. apiospermum were examined in vitro and in vivo.In culture, addition of the PRM mAbs increased S. apiospermum conidia germination and reduced conidial phagocytosis by J774.16 macrophages.Further insights into the effects of these glycopeptides on the pathobiology of S. apiospermum may lead to new avenues for preventing and treating scedosporiosis.

View Article: PubMed Central - PubMed

Affiliation: Departments of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, United States of America.

ABSTRACT
Scedosporium apiospermum is part of the Pseudallescheria-Scedosporium complex. Peptidorhamnomannans (PRMs) are cell wall glycopeptides present in some fungi, and their structures have been characterized in S. apiospermum, S. prolificans and Sporothrix schenckii. Prior work shows that PRMs can interact with host cells and that the glycopeptides are antigenic. In the present study, three monoclonal antibodies (mAbs, IgG1) to S. apiospermum derived PRM were generated and their effects on S. apiospermum were examined in vitro and in vivo. The mAbs recognized a carbohydrate epitope on PRM. In culture, addition of the PRM mAbs increased S. apiospermum conidia germination and reduced conidial phagocytosis by J774.16 macrophages. In a murine infection model, mice treated with antibodies to PRM died prior to control animals. Thus, PRM is involved in morphogenesis and the binding of this glycopeptide by mAbs enhanced the virulence of the fungus. Further insights into the effects of these glycopeptides on the pathobiology of S. apiospermum may lead to new avenues for preventing and treating scedosporiosis.

Show MeSH
Related in: MedlinePlus