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Atomic layer deposition-based functionalization of materials for medical and environmental health applications.

Narayan RJ, Adiga SP, Pellin MJ, Curtiss LA, Hryn AJ, Stafslien S, Chisholm B, Shih CC, Shih CM, Lin SJ, Su YY, Jin C, Zhang J, Monteiro-Riviere NA, Elam JW - Philos Trans A Math Phys Eng Sci (2010)

Bottom Line: In addition, films deposited by means of atomic layer deposition may impart improved biological functionality to nanoporous alumina membranes.PEGylated nanoporous alumina membranes were prepared by self-assembly of 1-mercaptoundec-11-yl hexa(ethylene glycol) on platinum-coated nanoporous alumina membranes.The results of this work indicate that nanoporous alumina membranes may be modified using atomic layer deposition for use in a variety of medical and environmental health applications.

View Article: PubMed Central - PubMed

Affiliation: Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, 2147 Burlington Engineering Labs, Raleigh, NC 27695-7115, USA. roger_narayan@msn.com

ABSTRACT
Nanoporous alumina membranes exhibit high pore densities, well-controlled and uniform pore sizes, as well as straight pores. Owing to these unusual properties, nanoporous alumina membranes are currently being considered for use in implantable sensor membranes and water purification membranes. Atomic layer deposition is a thin-film growth process that may be used to modify the pore size in a nanoporous alumina membrane while retaining a narrow pore distribution. In addition, films deposited by means of atomic layer deposition may impart improved biological functionality to nanoporous alumina membranes. In this study, zinc oxide coatings and platinum coatings were deposited on nanoporous alumina membranes by means of atomic layer deposition. PEGylated nanoporous alumina membranes were prepared by self-assembly of 1-mercaptoundec-11-yl hexa(ethylene glycol) on platinum-coated nanoporous alumina membranes. The pores of the PEGylated nanoporous alumina membranes remained free of fouling after exposure to human platelet-rich plasma; protein adsorption, fibrin networks and platelet aggregation were not observed on the coated membrane surface. Zinc oxide-coated nanoporous alumina membranes demonstrated activity against two waterborne pathogens, Escherichia coli and Staphylococcus aureus. The results of this work indicate that nanoporous alumina membranes may be modified using atomic layer deposition for use in a variety of medical and environmental health applications.

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The 24 h MTT viability assay data for the PEGylated, platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane, the platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane and the uncoated 20 nm pore size nanoporous alumina membrane. Data were standardized to the uncoated membrane control. The PEGylated, platinum-coated membrane and the platinum-coated membrane demonstrated lower viability than the uncoated membrane.
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RSTA20100011F6: The 24 h MTT viability assay data for the PEGylated, platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane, the platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane and the uncoated 20 nm pore size nanoporous alumina membrane. Data were standardized to the uncoated membrane control. The PEGylated, platinum-coated membrane and the platinum-coated membrane demonstrated lower viability than the uncoated membrane.

Mentions: Figure 4 shows a plan-view scanning electron micrograph of a platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane after self-assembly of the 1-mercaptoundec-11-yl hexa(ethylene glycol) monolayer. No particulates, blockages or other alterations in pore morphology were observed on the surface of the PEGylated, platinum-coated nanoporous alumina membrane. The self-assembly process allowed the nanoscale pores on the surface of the platinum-coated membrane to be retained. The FTIR spectrum of a PEGylated, platinum-coated and titanium-coated silicon wafer is shown in figure 5; this spectrum showed good correspondence with the spectra of self-assembled monolayers containing oligo(ethylene glycol) moieties that were previously examined by Zolk et al. (2000) and Wang et al. (2005). Symmetric CH2 stretching vibrations were shown to extend over a range from 2850 to 2950 cm−1. The peak 2920 cm−1 was assigned to asymmetric CH2 stretching vibrations from the ethylene glycol chain. Figure 6 shows the 24 h MTT viability assay data for the PEGylated, platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane, the platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane and the uncoated 20 nm pore size nanoporous alumina membrane. The PEGylated, platinum-coated nanoporous alumina membrane and the platinum-coated nanoporous alumina membrane exhibited a decrease in viability compared with the uncoated nanoporous alumina membrane. Hexa(ethylene glycol) and platinum have demonstrated good cell compatibility in previous studies by Pang (1993) and Bajaj et al. (2007), respectively. Recent work by Narayan et al. (2008) suggested that the metal coatings formed galvanic couples with residual metallic aluminium in the nanoporous alumina membranes coated using a line-of-sight process known as pulsed laser deposition; these interactions accelerated aluminium ion release and led to reduced cell viability rates.


Atomic layer deposition-based functionalization of materials for medical and environmental health applications.

Narayan RJ, Adiga SP, Pellin MJ, Curtiss LA, Hryn AJ, Stafslien S, Chisholm B, Shih CC, Shih CM, Lin SJ, Su YY, Jin C, Zhang J, Monteiro-Riviere NA, Elam JW - Philos Trans A Math Phys Eng Sci (2010)

The 24 h MTT viability assay data for the PEGylated, platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane, the platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane and the uncoated 20 nm pore size nanoporous alumina membrane. Data were standardized to the uncoated membrane control. The PEGylated, platinum-coated membrane and the platinum-coated membrane demonstrated lower viability than the uncoated membrane.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2944392&req=5

RSTA20100011F6: The 24 h MTT viability assay data for the PEGylated, platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane, the platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane and the uncoated 20 nm pore size nanoporous alumina membrane. Data were standardized to the uncoated membrane control. The PEGylated, platinum-coated membrane and the platinum-coated membrane demonstrated lower viability than the uncoated membrane.
Mentions: Figure 4 shows a plan-view scanning electron micrograph of a platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane after self-assembly of the 1-mercaptoundec-11-yl hexa(ethylene glycol) monolayer. No particulates, blockages or other alterations in pore morphology were observed on the surface of the PEGylated, platinum-coated nanoporous alumina membrane. The self-assembly process allowed the nanoscale pores on the surface of the platinum-coated membrane to be retained. The FTIR spectrum of a PEGylated, platinum-coated and titanium-coated silicon wafer is shown in figure 5; this spectrum showed good correspondence with the spectra of self-assembled monolayers containing oligo(ethylene glycol) moieties that were previously examined by Zolk et al. (2000) and Wang et al. (2005). Symmetric CH2 stretching vibrations were shown to extend over a range from 2850 to 2950 cm−1. The peak 2920 cm−1 was assigned to asymmetric CH2 stretching vibrations from the ethylene glycol chain. Figure 6 shows the 24 h MTT viability assay data for the PEGylated, platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane, the platinum-coated (coating=8 nm) 20 nm pore size nanoporous alumina membrane and the uncoated 20 nm pore size nanoporous alumina membrane. The PEGylated, platinum-coated nanoporous alumina membrane and the platinum-coated nanoporous alumina membrane exhibited a decrease in viability compared with the uncoated nanoporous alumina membrane. Hexa(ethylene glycol) and platinum have demonstrated good cell compatibility in previous studies by Pang (1993) and Bajaj et al. (2007), respectively. Recent work by Narayan et al. (2008) suggested that the metal coatings formed galvanic couples with residual metallic aluminium in the nanoporous alumina membranes coated using a line-of-sight process known as pulsed laser deposition; these interactions accelerated aluminium ion release and led to reduced cell viability rates.

Bottom Line: In addition, films deposited by means of atomic layer deposition may impart improved biological functionality to nanoporous alumina membranes.PEGylated nanoporous alumina membranes were prepared by self-assembly of 1-mercaptoundec-11-yl hexa(ethylene glycol) on platinum-coated nanoporous alumina membranes.The results of this work indicate that nanoporous alumina membranes may be modified using atomic layer deposition for use in a variety of medical and environmental health applications.

View Article: PubMed Central - PubMed

Affiliation: Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, 2147 Burlington Engineering Labs, Raleigh, NC 27695-7115, USA. roger_narayan@msn.com

ABSTRACT
Nanoporous alumina membranes exhibit high pore densities, well-controlled and uniform pore sizes, as well as straight pores. Owing to these unusual properties, nanoporous alumina membranes are currently being considered for use in implantable sensor membranes and water purification membranes. Atomic layer deposition is a thin-film growth process that may be used to modify the pore size in a nanoporous alumina membrane while retaining a narrow pore distribution. In addition, films deposited by means of atomic layer deposition may impart improved biological functionality to nanoporous alumina membranes. In this study, zinc oxide coatings and platinum coatings were deposited on nanoporous alumina membranes by means of atomic layer deposition. PEGylated nanoporous alumina membranes were prepared by self-assembly of 1-mercaptoundec-11-yl hexa(ethylene glycol) on platinum-coated nanoporous alumina membranes. The pores of the PEGylated nanoporous alumina membranes remained free of fouling after exposure to human platelet-rich plasma; protein adsorption, fibrin networks and platelet aggregation were not observed on the coated membrane surface. Zinc oxide-coated nanoporous alumina membranes demonstrated activity against two waterborne pathogens, Escherichia coli and Staphylococcus aureus. The results of this work indicate that nanoporous alumina membranes may be modified using atomic layer deposition for use in a variety of medical and environmental health applications.

Show MeSH
Related in: MedlinePlus