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Atomic layer deposition-based functionalization of materials for medical and environmental health applications.

Narayan RJ, Adiga SP, Pellin MJ, Curtiss LA, Hryn AJ, Stafslien S, Chisholm B, Shih CC, Shih CM, Lin SJ, Su YY, Jin C, Zhang J, Monteiro-Riviere NA, Elam JW - Philos Trans A Math Phys Eng Sci (2010)

Bottom Line: In addition, films deposited by means of atomic layer deposition may impart improved biological functionality to nanoporous alumina membranes.PEGylated nanoporous alumina membranes were prepared by self-assembly of 1-mercaptoundec-11-yl hexa(ethylene glycol) on platinum-coated nanoporous alumina membranes.The results of this work indicate that nanoporous alumina membranes may be modified using atomic layer deposition for use in a variety of medical and environmental health applications.

View Article: PubMed Central - PubMed

Affiliation: Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, 2147 Burlington Engineering Labs, Raleigh, NC 27695-7115, USA. roger_narayan@msn.com

ABSTRACT
Nanoporous alumina membranes exhibit high pore densities, well-controlled and uniform pore sizes, as well as straight pores. Owing to these unusual properties, nanoporous alumina membranes are currently being considered for use in implantable sensor membranes and water purification membranes. Atomic layer deposition is a thin-film growth process that may be used to modify the pore size in a nanoporous alumina membrane while retaining a narrow pore distribution. In addition, films deposited by means of atomic layer deposition may impart improved biological functionality to nanoporous alumina membranes. In this study, zinc oxide coatings and platinum coatings were deposited on nanoporous alumina membranes by means of atomic layer deposition. PEGylated nanoporous alumina membranes were prepared by self-assembly of 1-mercaptoundec-11-yl hexa(ethylene glycol) on platinum-coated nanoporous alumina membranes. The pores of the PEGylated nanoporous alumina membranes remained free of fouling after exposure to human platelet-rich plasma; protein adsorption, fibrin networks and platelet aggregation were not observed on the coated membrane surface. Zinc oxide-coated nanoporous alumina membranes demonstrated activity against two waterborne pathogens, Escherichia coli and Staphylococcus aureus. The results of this work indicate that nanoporous alumina membranes may be modified using atomic layer deposition for use in a variety of medical and environmental health applications.

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Light microscopy images of agar plating assay results after 24 h of incubation. Materials were examined on Luria–Bertani agar plates, which were inoculated with E. coli. (a) Uncoated 100 nm pore size nanoporous alumina membrane without light exposure. (b) Zinc oxide-coated (coating= 5 nm) 100 nm pore size nanoporous alumina membrane without light exposure. (c) Uncoated 100 nm pore size nanoporous alumina membrane under continuous light exposure. (d) Zinc oxide-coated (coating= 5 nm) 100 nm pore size nanoporous alumina membrane under continuous light exposure.
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RSTA20100011F15: Light microscopy images of agar plating assay results after 24 h of incubation. Materials were examined on Luria–Bertani agar plates, which were inoculated with E. coli. (a) Uncoated 100 nm pore size nanoporous alumina membrane without light exposure. (b) Zinc oxide-coated (coating= 5 nm) 100 nm pore size nanoporous alumina membrane without light exposure. (c) Uncoated 100 nm pore size nanoporous alumina membrane under continuous light exposure. (d) Zinc oxide-coated (coating= 5 nm) 100 nm pore size nanoporous alumina membrane under continuous light exposure.

Mentions: Agar plating assay results for the zinc oxide-coated 100 nm pore size nanoporous alumina membranes and uncoated nanoporous alumina membranes are shown in figures 15 and 16. Figure 15 shows light microscopy images of agar plating assay results after 24 h of incubation for uncoated and coated membranes, which were examined on Luria–Bertani agar plates inoculated with E. coli. Figure 16 shows light microscopy images of agar plating assay results after 24 h of incubation for uncoated and coated membranes, which were examined on tryptic soy agar plates inoculated with S. aureus. The uncoated nanoporous alumina membranes showed no inhibition of growth of E. coli and S. aureus under either continuous light or dark exposure. On the other hand, zinc oxide-coated nanoporous alumina membranes inhibited the growth of E. coli and S. aureus on the membrane surface. A small zone of growth inhibition was observed around each zinc oxide-coated membrane, indicating leaching of zinc oxide from the membrane. There was no discernable difference in antimicrobial performance between membranes examined under either continuous light or dark exposure.


Atomic layer deposition-based functionalization of materials for medical and environmental health applications.

Narayan RJ, Adiga SP, Pellin MJ, Curtiss LA, Hryn AJ, Stafslien S, Chisholm B, Shih CC, Shih CM, Lin SJ, Su YY, Jin C, Zhang J, Monteiro-Riviere NA, Elam JW - Philos Trans A Math Phys Eng Sci (2010)

Light microscopy images of agar plating assay results after 24 h of incubation. Materials were examined on Luria–Bertani agar plates, which were inoculated with E. coli. (a) Uncoated 100 nm pore size nanoporous alumina membrane without light exposure. (b) Zinc oxide-coated (coating= 5 nm) 100 nm pore size nanoporous alumina membrane without light exposure. (c) Uncoated 100 nm pore size nanoporous alumina membrane under continuous light exposure. (d) Zinc oxide-coated (coating= 5 nm) 100 nm pore size nanoporous alumina membrane under continuous light exposure.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2944392&req=5

RSTA20100011F15: Light microscopy images of agar plating assay results after 24 h of incubation. Materials were examined on Luria–Bertani agar plates, which were inoculated with E. coli. (a) Uncoated 100 nm pore size nanoporous alumina membrane without light exposure. (b) Zinc oxide-coated (coating= 5 nm) 100 nm pore size nanoporous alumina membrane without light exposure. (c) Uncoated 100 nm pore size nanoporous alumina membrane under continuous light exposure. (d) Zinc oxide-coated (coating= 5 nm) 100 nm pore size nanoporous alumina membrane under continuous light exposure.
Mentions: Agar plating assay results for the zinc oxide-coated 100 nm pore size nanoporous alumina membranes and uncoated nanoporous alumina membranes are shown in figures 15 and 16. Figure 15 shows light microscopy images of agar plating assay results after 24 h of incubation for uncoated and coated membranes, which were examined on Luria–Bertani agar plates inoculated with E. coli. Figure 16 shows light microscopy images of agar plating assay results after 24 h of incubation for uncoated and coated membranes, which were examined on tryptic soy agar plates inoculated with S. aureus. The uncoated nanoporous alumina membranes showed no inhibition of growth of E. coli and S. aureus under either continuous light or dark exposure. On the other hand, zinc oxide-coated nanoporous alumina membranes inhibited the growth of E. coli and S. aureus on the membrane surface. A small zone of growth inhibition was observed around each zinc oxide-coated membrane, indicating leaching of zinc oxide from the membrane. There was no discernable difference in antimicrobial performance between membranes examined under either continuous light or dark exposure.

Bottom Line: In addition, films deposited by means of atomic layer deposition may impart improved biological functionality to nanoporous alumina membranes.PEGylated nanoporous alumina membranes were prepared by self-assembly of 1-mercaptoundec-11-yl hexa(ethylene glycol) on platinum-coated nanoporous alumina membranes.The results of this work indicate that nanoporous alumina membranes may be modified using atomic layer deposition for use in a variety of medical and environmental health applications.

View Article: PubMed Central - PubMed

Affiliation: Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, 2147 Burlington Engineering Labs, Raleigh, NC 27695-7115, USA. roger_narayan@msn.com

ABSTRACT
Nanoporous alumina membranes exhibit high pore densities, well-controlled and uniform pore sizes, as well as straight pores. Owing to these unusual properties, nanoporous alumina membranes are currently being considered for use in implantable sensor membranes and water purification membranes. Atomic layer deposition is a thin-film growth process that may be used to modify the pore size in a nanoporous alumina membrane while retaining a narrow pore distribution. In addition, films deposited by means of atomic layer deposition may impart improved biological functionality to nanoporous alumina membranes. In this study, zinc oxide coatings and platinum coatings were deposited on nanoporous alumina membranes by means of atomic layer deposition. PEGylated nanoporous alumina membranes were prepared by self-assembly of 1-mercaptoundec-11-yl hexa(ethylene glycol) on platinum-coated nanoporous alumina membranes. The pores of the PEGylated nanoporous alumina membranes remained free of fouling after exposure to human platelet-rich plasma; protein adsorption, fibrin networks and platelet aggregation were not observed on the coated membrane surface. Zinc oxide-coated nanoporous alumina membranes demonstrated activity against two waterborne pathogens, Escherichia coli and Staphylococcus aureus. The results of this work indicate that nanoporous alumina membranes may be modified using atomic layer deposition for use in a variety of medical and environmental health applications.

Show MeSH
Related in: MedlinePlus