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Continuous differential impedance spectroscopy of single cells.

Malleo D, Nevill JT, Lee LP, Morgan H - Microfluid Nanofluidics (2009)

Bottom Line: Measurements are accomplished by recording the current from two closely-situated electrode pairs, one empty (reference) and one containing a cell.We demonstrate time-dependent measurement of single cell impedance produced in response to dynamic chemical perturbations.ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10404-009-0534-2) contains supplementary material, which is available to authorized users.

View Article: PubMed Central - PubMed

ABSTRACT
A device for continuous differential impedance analysis of single cells held by a hydrodynamic cell trapping is presented. Measurements are accomplished by recording the current from two closely-situated electrode pairs, one empty (reference) and one containing a cell. We demonstrate time-dependent measurement of single cell impedance produced in response to dynamic chemical perturbations. First, the system is used to assay the response of HeLa cells to the effects of the surfactant Tween, which reduces the impedance of the trapped cells in a concentration dependent way and is interpreted as gradual lysis of the cell membrane. Second, the effects of the bacterial pore-forming toxin, Streptolysin-O are measured: a transient exponential decay in the impedance is recorded as the cell membrane becomes increasingly permeable. The decay time constant is inversely proportional to toxin concentration (482, 150, and 30 s for 0.1, 1, and 10 kU/ml, respectively). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10404-009-0534-2) contains supplementary material, which is available to authorized users.

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Percent change in the magnitude of the impedance (f = 300 kHz) when cells are captured. Error bars represent one standard deviation for seven cells
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Fig4: Percent change in the magnitude of the impedance (f = 300 kHz) when cells are captured. Error bars represent one standard deviation for seven cells

Mentions: HeLa cells (suspended in PBS) flowing through the device were hydrodynamically captured, and the impedance change was measured. Figure 4 shows the average response for seven different single cells at a frequency of 300 kHz. Typical increases in /Zdiff/ ranged from 20 to 30%, consistent with simulation results (simulation data shown in Supplementary material). Error bars show one standard deviation, which is mostly attributed to cell-to-cell variability in addition to variations in the position of the cell within the trap.Fig. 4


Continuous differential impedance spectroscopy of single cells.

Malleo D, Nevill JT, Lee LP, Morgan H - Microfluid Nanofluidics (2009)

Percent change in the magnitude of the impedance (f = 300 kHz) when cells are captured. Error bars represent one standard deviation for seven cells
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2944380&req=5

Fig4: Percent change in the magnitude of the impedance (f = 300 kHz) when cells are captured. Error bars represent one standard deviation for seven cells
Mentions: HeLa cells (suspended in PBS) flowing through the device were hydrodynamically captured, and the impedance change was measured. Figure 4 shows the average response for seven different single cells at a frequency of 300 kHz. Typical increases in /Zdiff/ ranged from 20 to 30%, consistent with simulation results (simulation data shown in Supplementary material). Error bars show one standard deviation, which is mostly attributed to cell-to-cell variability in addition to variations in the position of the cell within the trap.Fig. 4

Bottom Line: Measurements are accomplished by recording the current from two closely-situated electrode pairs, one empty (reference) and one containing a cell.We demonstrate time-dependent measurement of single cell impedance produced in response to dynamic chemical perturbations.ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10404-009-0534-2) contains supplementary material, which is available to authorized users.

View Article: PubMed Central - PubMed

ABSTRACT
A device for continuous differential impedance analysis of single cells held by a hydrodynamic cell trapping is presented. Measurements are accomplished by recording the current from two closely-situated electrode pairs, one empty (reference) and one containing a cell. We demonstrate time-dependent measurement of single cell impedance produced in response to dynamic chemical perturbations. First, the system is used to assay the response of HeLa cells to the effects of the surfactant Tween, which reduces the impedance of the trapped cells in a concentration dependent way and is interpreted as gradual lysis of the cell membrane. Second, the effects of the bacterial pore-forming toxin, Streptolysin-O are measured: a transient exponential decay in the impedance is recorded as the cell membrane becomes increasingly permeable. The decay time constant is inversely proportional to toxin concentration (482, 150, and 30 s for 0.1, 1, and 10 kU/ml, respectively). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10404-009-0534-2) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus