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Cellularity and adipogenic profile of the abdominal subcutaneous adipose tissue from obese adolescents: association with insulin resistance and hepatic steatosis.

Kursawe R, Eszlinger M, Narayan D, Liu T, Bazuine M, Cali AM, D'Adamo E, Shaw M, Pierpont B, Shulman GI, Cushman SW, Sherman A, Caprio S - Diabetes (2010)

Bottom Line: The cell-size distribution curves were significantly different between the high and low VAT/(VAT+SAT) groups, even after adjusting for age, sex, and ethnicity (MANOVA P = 0.035).Surprisingly, the fraction of large adipocytes was significantly lower (P < 0.01) in the group with high VAT/(VAT+SAT), along with the estimated total number of large adipose cells (P < 0.05), while the mean diameter was increased (P < 0.01).A reduced lipo-/adipogenic capacity, fraction, and estimated number of large subcutaneous adipocytes may contribute to the abnormal distribution of abdominal fat and hepatic steatosis, as well as to insulin resistance in obese adolescents.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut, USA.

ABSTRACT

Objective: We explored whether the distribution of adipose cell size, the estimated total number of adipose cells, and the expression of adipogenic genes in subcutaneous adipose tissue are linked to the phenotype of high visceral and low subcutaneous fat depots in obese adolescents.

Research design and methods: A total of 38 adolescents with similar degrees of obesity agreed to have a subcutaneous periumbilical adipose tissue biopsy, in addition to metabolic (oral glucose tolerance test and hyperinsulinemic euglycemic clamp) and imaging studies (MRI, DEXA, (1)H-NMR). Subcutaneous periumbilical adipose cell-size distribution and the estimated total number of subcutaneous adipose cells were obtained from tissue biopsy samples fixed in osmium tetroxide and analyzed by Beckman Coulter Multisizer. The adipogenic capacity was measured by Affymetrix GeneChip and quantitative RT-PCR.

Results: Subjects were divided into two groups: high versus low ratio of visceral to visceral + subcutaneous fat (VAT/[VAT+SAT]). The cell-size distribution curves were significantly different between the high and low VAT/(VAT+SAT) groups, even after adjusting for age, sex, and ethnicity (MANOVA P = 0.035). Surprisingly, the fraction of large adipocytes was significantly lower (P < 0.01) in the group with high VAT/(VAT+SAT), along with the estimated total number of large adipose cells (P < 0.05), while the mean diameter was increased (P < 0.01). From the microarray analyses emerged a lower expression of lipogenesis/adipogenesis markers (sterol regulatory element binding protein-1, acetyl-CoA carboxylase, fatty acid synthase) in the group with high VAT/(VAT+SAT), which was confirmed by RT-PCR.

Conclusions: A reduced lipo-/adipogenic capacity, fraction, and estimated number of large subcutaneous adipocytes may contribute to the abnormal distribution of abdominal fat and hepatic steatosis, as well as to insulin resistance in obese adolescents.

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Related in: MedlinePlus

Multisizer adipose cell profiles of 20 subjects with a low VAT/(VAT+SAT) ratio (A) and 18 subjects with a high VAT/(VAT+SAT) ratio (B), plotting cell diameter using linear bins against relative frequency in percent C: Cell-size profiles of the adipose cell size using the mean parameters from the curve-fitting formula for subjects with a low VAT/(VAT+SAT) ratio (dashed line) and subjects with a high VAT/(VAT+SAT) ratio (solid line) (P = 0.035 using MANCOVA).
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Figure 1: Multisizer adipose cell profiles of 20 subjects with a low VAT/(VAT+SAT) ratio (A) and 18 subjects with a high VAT/(VAT+SAT) ratio (B), plotting cell diameter using linear bins against relative frequency in percent C: Cell-size profiles of the adipose cell size using the mean parameters from the curve-fitting formula for subjects with a low VAT/(VAT+SAT) ratio (dashed line) and subjects with a high VAT/(VAT+SAT) ratio (solid line) (P = 0.035 using MANCOVA).

Mentions: Fig. 1A and B illustrates the adipose cell-size distribution profile for each individual in the low and high ratio groups, and Fig. 1C shows representative adipose cell-size profiles for the two groups using the average parameters determined by the curve-fitting analyses. The two curves are distinctly different, showing a shift to the right in those with a high ratio. MANOVA analysis applied to the seven curve-fitting parameters showed that the overall cell-size distribution was statistically significantly different between the low and high VAT/(VAT+SAT) groups (P = 0.027). After adjusting for age, BMI, sex, and ethnicity, MANCOVA analysis continued to show a statistically significant difference (P = 0.035).


Cellularity and adipogenic profile of the abdominal subcutaneous adipose tissue from obese adolescents: association with insulin resistance and hepatic steatosis.

Kursawe R, Eszlinger M, Narayan D, Liu T, Bazuine M, Cali AM, D'Adamo E, Shaw M, Pierpont B, Shulman GI, Cushman SW, Sherman A, Caprio S - Diabetes (2010)

Multisizer adipose cell profiles of 20 subjects with a low VAT/(VAT+SAT) ratio (A) and 18 subjects with a high VAT/(VAT+SAT) ratio (B), plotting cell diameter using linear bins against relative frequency in percent C: Cell-size profiles of the adipose cell size using the mean parameters from the curve-fitting formula for subjects with a low VAT/(VAT+SAT) ratio (dashed line) and subjects with a high VAT/(VAT+SAT) ratio (solid line) (P = 0.035 using MANCOVA).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2927952&req=5

Figure 1: Multisizer adipose cell profiles of 20 subjects with a low VAT/(VAT+SAT) ratio (A) and 18 subjects with a high VAT/(VAT+SAT) ratio (B), plotting cell diameter using linear bins against relative frequency in percent C: Cell-size profiles of the adipose cell size using the mean parameters from the curve-fitting formula for subjects with a low VAT/(VAT+SAT) ratio (dashed line) and subjects with a high VAT/(VAT+SAT) ratio (solid line) (P = 0.035 using MANCOVA).
Mentions: Fig. 1A and B illustrates the adipose cell-size distribution profile for each individual in the low and high ratio groups, and Fig. 1C shows representative adipose cell-size profiles for the two groups using the average parameters determined by the curve-fitting analyses. The two curves are distinctly different, showing a shift to the right in those with a high ratio. MANOVA analysis applied to the seven curve-fitting parameters showed that the overall cell-size distribution was statistically significantly different between the low and high VAT/(VAT+SAT) groups (P = 0.027). After adjusting for age, BMI, sex, and ethnicity, MANCOVA analysis continued to show a statistically significant difference (P = 0.035).

Bottom Line: The cell-size distribution curves were significantly different between the high and low VAT/(VAT+SAT) groups, even after adjusting for age, sex, and ethnicity (MANOVA P = 0.035).Surprisingly, the fraction of large adipocytes was significantly lower (P < 0.01) in the group with high VAT/(VAT+SAT), along with the estimated total number of large adipose cells (P < 0.05), while the mean diameter was increased (P < 0.01).A reduced lipo-/adipogenic capacity, fraction, and estimated number of large subcutaneous adipocytes may contribute to the abnormal distribution of abdominal fat and hepatic steatosis, as well as to insulin resistance in obese adolescents.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut, USA.

ABSTRACT

Objective: We explored whether the distribution of adipose cell size, the estimated total number of adipose cells, and the expression of adipogenic genes in subcutaneous adipose tissue are linked to the phenotype of high visceral and low subcutaneous fat depots in obese adolescents.

Research design and methods: A total of 38 adolescents with similar degrees of obesity agreed to have a subcutaneous periumbilical adipose tissue biopsy, in addition to metabolic (oral glucose tolerance test and hyperinsulinemic euglycemic clamp) and imaging studies (MRI, DEXA, (1)H-NMR). Subcutaneous periumbilical adipose cell-size distribution and the estimated total number of subcutaneous adipose cells were obtained from tissue biopsy samples fixed in osmium tetroxide and analyzed by Beckman Coulter Multisizer. The adipogenic capacity was measured by Affymetrix GeneChip and quantitative RT-PCR.

Results: Subjects were divided into two groups: high versus low ratio of visceral to visceral + subcutaneous fat (VAT/[VAT+SAT]). The cell-size distribution curves were significantly different between the high and low VAT/(VAT+SAT) groups, even after adjusting for age, sex, and ethnicity (MANOVA P = 0.035). Surprisingly, the fraction of large adipocytes was significantly lower (P < 0.01) in the group with high VAT/(VAT+SAT), along with the estimated total number of large adipose cells (P < 0.05), while the mean diameter was increased (P < 0.01). From the microarray analyses emerged a lower expression of lipogenesis/adipogenesis markers (sterol regulatory element binding protein-1, acetyl-CoA carboxylase, fatty acid synthase) in the group with high VAT/(VAT+SAT), which was confirmed by RT-PCR.

Conclusions: A reduced lipo-/adipogenic capacity, fraction, and estimated number of large subcutaneous adipocytes may contribute to the abnormal distribution of abdominal fat and hepatic steatosis, as well as to insulin resistance in obese adolescents.

Show MeSH
Related in: MedlinePlus