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Glucose-dependent insulinotropic polypeptide may enhance fatty acid re-esterification in subcutaneous abdominal adipose tissue in lean humans.

Asmar M, Simonsen L, Madsbad S, Stallknecht B, Holst JJ, Bülow J - Diabetes (2010)

Bottom Line: In humans, however, there is no clear evidence of GIP effecting lipid metabolism.The present experiments were performed in order to elucidate the effects of GIP on regional adipose tissue metabolism.During GIP and HI-HG clamp, abdominal subcutaneous adipose tissue blood flow, hydrolysis of circulating triacylglycerol (TAG) (P = 0.009), and glucose uptake (P = 0.03) increased significantly while free fatty acid (FFA) output (P = 0.04) and FFA/glycerol release ratio (P = 0.02) decreased compared with saline and HI-HG clamp.

View Article: PubMed Central - PubMed

Affiliation: 1Department of Clinical Physiology/Nuclear Medicine, Bispebjerg Hospital, Copenhagen, Denmark.

ABSTRACT

Objective: Glucose-dependent insulinotropic polypeptide (GIP) has been implicated in lipid metabolism in animals. In humans, however, there is no clear evidence of GIP effecting lipid metabolism. The present experiments were performed in order to elucidate the effects of GIP on regional adipose tissue metabolism.

Research design and methods: Eight healthy subjects were studied on four different occasions. Abdominal subcutaneous adipose tissue metabolism was assessed by measuring arterio-venous concentration differences and regional adipose tissue blood flow during GIP (1.5 pmol/kg/min) or saline infused intravenously alone or in combination with a hyperinsulinemic-hyperglycemic (HI-HG) clamp.

Results: During GIP and HI-HG clamp, abdominal subcutaneous adipose tissue blood flow, hydrolysis of circulating triacylglycerol (TAG) (P = 0.009), and glucose uptake (P = 0.03) increased significantly while free fatty acid (FFA) output (P = 0.04) and FFA/glycerol release ratio (P = 0.02) decreased compared with saline and HI-HG clamp.

Conclusions: In conclusion, GIP in combination with hyperinsulinemia and slight hyperglycemia increased adipose tissue blood flow, glucose uptake, and FFA re-esterification, thus resulting in increased TAG deposition in abdominal subcutaneous adipose tissue.

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Data are means ± SEM. Adipose tissue blood flow during HI-HG clamp experiment with GIP (▴) and without GIP (△), GIP alone (●), or saline alone (○).
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Figure 3: Data are means ± SEM. Adipose tissue blood flow during HI-HG clamp experiment with GIP (▴) and without GIP (△), GIP alone (●), or saline alone (○).

Mentions: Figure 3 shows the subcutaneous abdominal adipose tissue blood flow during the four experiments. During the GIP and HI-HG clamp experiment, ATBF increased significantly during the first 30 min, reached a plateau of 3.9 ± 0.4 versus 1.4 ± 0.1 ml · min−1 · (100 g tissue)−1 after 90 min, and differed significantly from the saline and HI-HG clamp experiment (1.4 ± 0.1 ml · min−1 · [100 g tissue]−1, P < 0.0001). During the saline and HI-HG clamp experiment, ATBF remained virtually constant with a tendency toward a slight decrease at the end of the experiment. During GIP alone, a slight but significant ATBF increase was seen during the first 60 min when compared with saline (1.6 ± 0.02 vs. 1.1 ± 0.01 ml · [100 g tissue]−1 · [60 min]−1, respectively; P = 0.04) concomitant with elevated insulin levels. After 60 min, ATBF was similar during the two experiments and increased steadily and differed significantly from ATBF during the HI-HG clamp experiment without GIP during the last hour (P < 0.05).


Glucose-dependent insulinotropic polypeptide may enhance fatty acid re-esterification in subcutaneous abdominal adipose tissue in lean humans.

Asmar M, Simonsen L, Madsbad S, Stallknecht B, Holst JJ, Bülow J - Diabetes (2010)

Data are means ± SEM. Adipose tissue blood flow during HI-HG clamp experiment with GIP (▴) and without GIP (△), GIP alone (●), or saline alone (○).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2927937&req=5

Figure 3: Data are means ± SEM. Adipose tissue blood flow during HI-HG clamp experiment with GIP (▴) and without GIP (△), GIP alone (●), or saline alone (○).
Mentions: Figure 3 shows the subcutaneous abdominal adipose tissue blood flow during the four experiments. During the GIP and HI-HG clamp experiment, ATBF increased significantly during the first 30 min, reached a plateau of 3.9 ± 0.4 versus 1.4 ± 0.1 ml · min−1 · (100 g tissue)−1 after 90 min, and differed significantly from the saline and HI-HG clamp experiment (1.4 ± 0.1 ml · min−1 · [100 g tissue]−1, P < 0.0001). During the saline and HI-HG clamp experiment, ATBF remained virtually constant with a tendency toward a slight decrease at the end of the experiment. During GIP alone, a slight but significant ATBF increase was seen during the first 60 min when compared with saline (1.6 ± 0.02 vs. 1.1 ± 0.01 ml · [100 g tissue]−1 · [60 min]−1, respectively; P = 0.04) concomitant with elevated insulin levels. After 60 min, ATBF was similar during the two experiments and increased steadily and differed significantly from ATBF during the HI-HG clamp experiment without GIP during the last hour (P < 0.05).

Bottom Line: In humans, however, there is no clear evidence of GIP effecting lipid metabolism.The present experiments were performed in order to elucidate the effects of GIP on regional adipose tissue metabolism.During GIP and HI-HG clamp, abdominal subcutaneous adipose tissue blood flow, hydrolysis of circulating triacylglycerol (TAG) (P = 0.009), and glucose uptake (P = 0.03) increased significantly while free fatty acid (FFA) output (P = 0.04) and FFA/glycerol release ratio (P = 0.02) decreased compared with saline and HI-HG clamp.

View Article: PubMed Central - PubMed

Affiliation: 1Department of Clinical Physiology/Nuclear Medicine, Bispebjerg Hospital, Copenhagen, Denmark.

ABSTRACT

Objective: Glucose-dependent insulinotropic polypeptide (GIP) has been implicated in lipid metabolism in animals. In humans, however, there is no clear evidence of GIP effecting lipid metabolism. The present experiments were performed in order to elucidate the effects of GIP on regional adipose tissue metabolism.

Research design and methods: Eight healthy subjects were studied on four different occasions. Abdominal subcutaneous adipose tissue metabolism was assessed by measuring arterio-venous concentration differences and regional adipose tissue blood flow during GIP (1.5 pmol/kg/min) or saline infused intravenously alone or in combination with a hyperinsulinemic-hyperglycemic (HI-HG) clamp.

Results: During GIP and HI-HG clamp, abdominal subcutaneous adipose tissue blood flow, hydrolysis of circulating triacylglycerol (TAG) (P = 0.009), and glucose uptake (P = 0.03) increased significantly while free fatty acid (FFA) output (P = 0.04) and FFA/glycerol release ratio (P = 0.02) decreased compared with saline and HI-HG clamp.

Conclusions: In conclusion, GIP in combination with hyperinsulinemia and slight hyperglycemia increased adipose tissue blood flow, glucose uptake, and FFA re-esterification, thus resulting in increased TAG deposition in abdominal subcutaneous adipose tissue.

Show MeSH
Related in: MedlinePlus