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Glucose-dependent insulinotropic polypeptide may enhance fatty acid re-esterification in subcutaneous abdominal adipose tissue in lean humans.

Asmar M, Simonsen L, Madsbad S, Stallknecht B, Holst JJ, Bülow J - Diabetes (2010)

Bottom Line: In humans, however, there is no clear evidence of GIP effecting lipid metabolism.The present experiments were performed in order to elucidate the effects of GIP on regional adipose tissue metabolism.During GIP and HI-HG clamp, abdominal subcutaneous adipose tissue blood flow, hydrolysis of circulating triacylglycerol (TAG) (P = 0.009), and glucose uptake (P = 0.03) increased significantly while free fatty acid (FFA) output (P = 0.04) and FFA/glycerol release ratio (P = 0.02) decreased compared with saline and HI-HG clamp.

View Article: PubMed Central - PubMed

Affiliation: 1Department of Clinical Physiology/Nuclear Medicine, Bispebjerg Hospital, Copenhagen, Denmark.

ABSTRACT

Objective: Glucose-dependent insulinotropic polypeptide (GIP) has been implicated in lipid metabolism in animals. In humans, however, there is no clear evidence of GIP effecting lipid metabolism. The present experiments were performed in order to elucidate the effects of GIP on regional adipose tissue metabolism.

Research design and methods: Eight healthy subjects were studied on four different occasions. Abdominal subcutaneous adipose tissue metabolism was assessed by measuring arterio-venous concentration differences and regional adipose tissue blood flow during GIP (1.5 pmol/kg/min) or saline infused intravenously alone or in combination with a hyperinsulinemic-hyperglycemic (HI-HG) clamp.

Results: During GIP and HI-HG clamp, abdominal subcutaneous adipose tissue blood flow, hydrolysis of circulating triacylglycerol (TAG) (P = 0.009), and glucose uptake (P = 0.03) increased significantly while free fatty acid (FFA) output (P = 0.04) and FFA/glycerol release ratio (P = 0.02) decreased compared with saline and HI-HG clamp.

Conclusions: In conclusion, GIP in combination with hyperinsulinemia and slight hyperglycemia increased adipose tissue blood flow, glucose uptake, and FFA re-esterification, thus resulting in increased TAG deposition in abdominal subcutaneous adipose tissue.

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Related in: MedlinePlus

Plasma arterial TAG (A), FFA (B), and glycerol (C) concentrations during HI-HG clamp experiment with GIP (▴) and without GIP (△), GIP alone (●), or saline alone (○).
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Figure 2: Plasma arterial TAG (A), FFA (B), and glycerol (C) concentrations during HI-HG clamp experiment with GIP (▴) and without GIP (△), GIP alone (●), or saline alone (○).

Mentions: The arterial concentrations of the metabolites during the four experiments are given in Fig. 2. During the HI-HG clamp experiments with and without GIP, arterial TAG, FFA, and glycerol concentrations decreased significantly with no differences seen between the two experiments. During GIP or saline alone, arterial TAG concentrations remained constant and no differences were seen between the two experiments. Arterial FFA and glycerol concentrations did not differ significantly during saline and GIP infusions alone and increased steadily during the 300 min.


Glucose-dependent insulinotropic polypeptide may enhance fatty acid re-esterification in subcutaneous abdominal adipose tissue in lean humans.

Asmar M, Simonsen L, Madsbad S, Stallknecht B, Holst JJ, Bülow J - Diabetes (2010)

Plasma arterial TAG (A), FFA (B), and glycerol (C) concentrations during HI-HG clamp experiment with GIP (▴) and without GIP (△), GIP alone (●), or saline alone (○).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2927937&req=5

Figure 2: Plasma arterial TAG (A), FFA (B), and glycerol (C) concentrations during HI-HG clamp experiment with GIP (▴) and without GIP (△), GIP alone (●), or saline alone (○).
Mentions: The arterial concentrations of the metabolites during the four experiments are given in Fig. 2. During the HI-HG clamp experiments with and without GIP, arterial TAG, FFA, and glycerol concentrations decreased significantly with no differences seen between the two experiments. During GIP or saline alone, arterial TAG concentrations remained constant and no differences were seen between the two experiments. Arterial FFA and glycerol concentrations did not differ significantly during saline and GIP infusions alone and increased steadily during the 300 min.

Bottom Line: In humans, however, there is no clear evidence of GIP effecting lipid metabolism.The present experiments were performed in order to elucidate the effects of GIP on regional adipose tissue metabolism.During GIP and HI-HG clamp, abdominal subcutaneous adipose tissue blood flow, hydrolysis of circulating triacylglycerol (TAG) (P = 0.009), and glucose uptake (P = 0.03) increased significantly while free fatty acid (FFA) output (P = 0.04) and FFA/glycerol release ratio (P = 0.02) decreased compared with saline and HI-HG clamp.

View Article: PubMed Central - PubMed

Affiliation: 1Department of Clinical Physiology/Nuclear Medicine, Bispebjerg Hospital, Copenhagen, Denmark.

ABSTRACT

Objective: Glucose-dependent insulinotropic polypeptide (GIP) has been implicated in lipid metabolism in animals. In humans, however, there is no clear evidence of GIP effecting lipid metabolism. The present experiments were performed in order to elucidate the effects of GIP on regional adipose tissue metabolism.

Research design and methods: Eight healthy subjects were studied on four different occasions. Abdominal subcutaneous adipose tissue metabolism was assessed by measuring arterio-venous concentration differences and regional adipose tissue blood flow during GIP (1.5 pmol/kg/min) or saline infused intravenously alone or in combination with a hyperinsulinemic-hyperglycemic (HI-HG) clamp.

Results: During GIP and HI-HG clamp, abdominal subcutaneous adipose tissue blood flow, hydrolysis of circulating triacylglycerol (TAG) (P = 0.009), and glucose uptake (P = 0.03) increased significantly while free fatty acid (FFA) output (P = 0.04) and FFA/glycerol release ratio (P = 0.02) decreased compared with saline and HI-HG clamp.

Conclusions: In conclusion, GIP in combination with hyperinsulinemia and slight hyperglycemia increased adipose tissue blood flow, glucose uptake, and FFA re-esterification, thus resulting in increased TAG deposition in abdominal subcutaneous adipose tissue.

Show MeSH
Related in: MedlinePlus