Limits...
Recent advances and prospects in the differentiation of pancreatic cells from human embryonic stem cells.

Mfopou JK, Chen B, Sui L, Sermon K, Bouwens L - Diabetes (2010)

Bottom Line: Recent studies with human embryonic stem (hES) cells have established new protocols for substantial generation of pancreatic progenitors from definitive endoderm.It also indicates that critical refining of the available strategies might help define a universal protocol for pancreatic differentiation applicable to several cell lines, therefore offering the possibility for transplantation of immune-matched or patient-specific hES-derived beta-cells.We appraise here the fundamental role that bone morphogenetic protein, fibroblast growth factor, and retinoid signaling play during pancreas development, and describe a fundamental emergence of their combination in recent studies that generated pancreatic cells from hES cells.

View Article: PubMed Central - PubMed

Affiliation: Cell Differentiation Unit, Diabetes Research Centre, Vrije Universiteit Brussel, Brussels, Belgium.

ABSTRACT
Recent studies with human embryonic stem (hES) cells have established new protocols for substantial generation of pancreatic progenitors from definitive endoderm. These findings add to the efficient derivation of definitive endoderm, which is controlled by Wnt and Nodal pathways, and delineate a step forward in the quest for alternative beta-cell sources. It also indicates that critical refining of the available strategies might help define a universal protocol for pancreatic differentiation applicable to several cell lines, therefore offering the possibility for transplantation of immune-matched or patient-specific hES-derived beta-cells. We appraise here the fundamental role that bone morphogenetic protein, fibroblast growth factor, and retinoid signaling play during pancreas development, and describe a fundamental emergence of their combination in recent studies that generated pancreatic cells from hES cells. We finally enumerate some prospects that might improve further differentiation of the progenitor cells into functional beta-cells needed in diabetes cell therapy.

Show MeSH

Related in: MedlinePlus

Overview of transcription factors (TFs) expression during β-cell development. Expression of different combinations of TFs determines the sequential lineage segregation in the developing pancreas. Not only are the combinations of TFs important, but the expression level of a particular TF is known to guide differentiation as well. Low protein levels are indicated in parentheses. The most relevant TFs that are landmarks for the selection of each stage/lineage are shown.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2927928&req=5

Figure 1: Overview of transcription factors (TFs) expression during β-cell development. Expression of different combinations of TFs determines the sequential lineage segregation in the developing pancreas. Not only are the combinations of TFs important, but the expression level of a particular TF is known to guide differentiation as well. Low protein levels are indicated in parentheses. The most relevant TFs that are landmarks for the selection of each stage/lineage are shown.

Mentions: During the past two decennia, considerable progress has been achieved in understanding pancreas embryogenesis thanks to the numerous genetic studies involved in transcription factors. Most of them belong to the homeobox domain, the paired-box, or the basic helix-loop-helix families. The sequential expression of different transcription factors that control pancreas development has been extensively reviewed elsewhere (6–8,10). Only a brief summary of their expression pattern is given in Fig. 1 with special focus on 1) the early pancreatic epithelial cells, 2) the pancreatic endocrine progenitors, and 3) the endocrine subtypes selection.


Recent advances and prospects in the differentiation of pancreatic cells from human embryonic stem cells.

Mfopou JK, Chen B, Sui L, Sermon K, Bouwens L - Diabetes (2010)

Overview of transcription factors (TFs) expression during β-cell development. Expression of different combinations of TFs determines the sequential lineage segregation in the developing pancreas. Not only are the combinations of TFs important, but the expression level of a particular TF is known to guide differentiation as well. Low protein levels are indicated in parentheses. The most relevant TFs that are landmarks for the selection of each stage/lineage are shown.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2927928&req=5

Figure 1: Overview of transcription factors (TFs) expression during β-cell development. Expression of different combinations of TFs determines the sequential lineage segregation in the developing pancreas. Not only are the combinations of TFs important, but the expression level of a particular TF is known to guide differentiation as well. Low protein levels are indicated in parentheses. The most relevant TFs that are landmarks for the selection of each stage/lineage are shown.
Mentions: During the past two decennia, considerable progress has been achieved in understanding pancreas embryogenesis thanks to the numerous genetic studies involved in transcription factors. Most of them belong to the homeobox domain, the paired-box, or the basic helix-loop-helix families. The sequential expression of different transcription factors that control pancreas development has been extensively reviewed elsewhere (6–8,10). Only a brief summary of their expression pattern is given in Fig. 1 with special focus on 1) the early pancreatic epithelial cells, 2) the pancreatic endocrine progenitors, and 3) the endocrine subtypes selection.

Bottom Line: Recent studies with human embryonic stem (hES) cells have established new protocols for substantial generation of pancreatic progenitors from definitive endoderm.It also indicates that critical refining of the available strategies might help define a universal protocol for pancreatic differentiation applicable to several cell lines, therefore offering the possibility for transplantation of immune-matched or patient-specific hES-derived beta-cells.We appraise here the fundamental role that bone morphogenetic protein, fibroblast growth factor, and retinoid signaling play during pancreas development, and describe a fundamental emergence of their combination in recent studies that generated pancreatic cells from hES cells.

View Article: PubMed Central - PubMed

Affiliation: Cell Differentiation Unit, Diabetes Research Centre, Vrije Universiteit Brussel, Brussels, Belgium.

ABSTRACT
Recent studies with human embryonic stem (hES) cells have established new protocols for substantial generation of pancreatic progenitors from definitive endoderm. These findings add to the efficient derivation of definitive endoderm, which is controlled by Wnt and Nodal pathways, and delineate a step forward in the quest for alternative beta-cell sources. It also indicates that critical refining of the available strategies might help define a universal protocol for pancreatic differentiation applicable to several cell lines, therefore offering the possibility for transplantation of immune-matched or patient-specific hES-derived beta-cells. We appraise here the fundamental role that bone morphogenetic protein, fibroblast growth factor, and retinoid signaling play during pancreas development, and describe a fundamental emergence of their combination in recent studies that generated pancreatic cells from hES cells. We finally enumerate some prospects that might improve further differentiation of the progenitor cells into functional beta-cells needed in diabetes cell therapy.

Show MeSH
Related in: MedlinePlus