Contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content.
Bottom Line: Leukocytes invade newly formed thrombi through interactions with platelets and fibrin and later contribute to the removal of fibrin deposits mainly through the action of neutrophil elastase.The digitalized fluorescent microscopic images were decomposed according to the color channel of each thrombus constituent.Association between NE-FDP and leukocyte content of thrombi is evidenced by a significant Pearson correlation coefficient of 0.71 (p=0.00002).
Affiliation: Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary.Show MeSH
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Mentions: SEM images of thrombi provided evidence of leukocytes forming pseudopodia in-between the fibrin fibers (Fig. 2A). Leukocytes could be observed in tight proximity with platelets and differences in pseudopodium formation indicated that they are in different stages of activation (Fig. 2B). Because SEM imaging allows only qualitative morphological evaluation of the interrelations between leukocytes, platelets and fibrin, the functional aspects of leukocyte presence with respect to thrombus dissolution were approached with quantitative data on neutrophil-related fibrin digestion.
Affiliation: Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary.