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Detection of viral antigen, IgM and IgG antibodies in cerebrospinal fluid of Chikungunya patients with neurological complications.

Kashyap RS, Morey SH, Chandak NH, Purohit HJ, Taori GM, Daginawala HF - Cerebrospinal Fluid Res (2010)

Bottom Line: Early and cost-effective diagnosis of these patients remains problematic despite many new advanced diagnostic methods.A reliable diagnostic test, which could be performed in any standard pathology laboratory, would help to obtain definitive early diagnosis of CHIKV patients with neurological complications.The sensitivity for detection of IgM 48% or IgG 63% was significantly lower than the antigen assay (80%).

View Article: PubMed Central - HTML - PubMed

Affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, 88/2, Bajaj Nagar, Nagpur 440010, India. hfd_ciims@rediffmail.com.

ABSTRACT

Background: During Chikungunya virus (CHIKV) epidemic in Nagpur, India, we identified some suspected Chikungunya patients with neurological complications. Early and cost-effective diagnosis of these patients remains problematic despite many new advanced diagnostic methods. A reliable diagnostic test, which could be performed in any standard pathology laboratory, would help to obtain definitive early diagnosis of CHIKV patients with neurological complications. In our laboratory, in-house ELISA protocol for viral antigen, immunoglobulin M (IgM) and IgG detection has been developed and assessed for the diagnosis of CHIKV patients with neurological complications.

Method: Cerebrospinal fluid samples of forty-six patients who developed neurological symptoms within two months of CHIKV infections along with control subjects were included in the study and were analyzed for the presence of antigens and of IgM and IgG using an ELISA protocol.

Results: The ELISA method for antigen detection yielded 80% sensitivity and 87% specificity for the diagnosis of CHIKV patients with neurological complications. The sensitivity for detection of IgM 48% or IgG 63% was significantly lower than the antigen assay (80%).

Conclusion: The detection of viral antigen in CSF of CHIKV patients with neurological complications by ELISA method gave a more reliable diagnosis than antibodies detection that can be used to develop an immunodiagnostic assay with increased sensitivity and specificity.

No MeSH data available.


Related in: MedlinePlus

Mean absorbance values of ELISA assays for a) CHIKV viral antigen, b) CHIKV IgM, c) CHIKV IgG in CSF from patients with confirmed (n = 9) and suspected CHIKV (n = 37) with neurological complications and patients from non-CHIKV group (n = 15). Data are means +/- SD. * P < 0.05, ** P < 0.01 relative to control non-CHIKV subjects.
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Figure 1: Mean absorbance values of ELISA assays for a) CHIKV viral antigen, b) CHIKV IgM, c) CHIKV IgG in CSF from patients with confirmed (n = 9) and suspected CHIKV (n = 37) with neurological complications and patients from non-CHIKV group (n = 15). Data are means +/- SD. * P < 0.05, ** P < 0.01 relative to control non-CHIKV subjects.

Mentions: The CSF positivities for CHIKV antigen in cases of confirmed neuro-CHIKV and suspected patients were 100% (9/9) and 76% (28/37) respectively, while the positivity for patients in the Non-CHIKV group was 13% (02/15) (Table 1). The mean absorbance value of CHIKV antigen in CSF from the CHIKV and non CHIKV groups as determined by the ELISA method is shown in Figure 1A. The mean absorbance value of CHIKV antigen in the confirmed neuro-CHIKV patients was 0.84 ± 0.19 and for suspected Chikungunya patients was 0.64 ± 0.19 which were significantly higher than the non CHIKV group (0.36 ± 0.08; P < 0.001). There was a significant difference in the mean CHIKV antigen activity between the confirmed neuro-CHIKV patients (0.84 ± 0.23) and the suspected-CHIKV patients (0.59 ± 0.14; P < 0.05).


Detection of viral antigen, IgM and IgG antibodies in cerebrospinal fluid of Chikungunya patients with neurological complications.

Kashyap RS, Morey SH, Chandak NH, Purohit HJ, Taori GM, Daginawala HF - Cerebrospinal Fluid Res (2010)

Mean absorbance values of ELISA assays for a) CHIKV viral antigen, b) CHIKV IgM, c) CHIKV IgG in CSF from patients with confirmed (n = 9) and suspected CHIKV (n = 37) with neurological complications and patients from non-CHIKV group (n = 15). Data are means +/- SD. * P < 0.05, ** P < 0.01 relative to control non-CHIKV subjects.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2927496&req=5

Figure 1: Mean absorbance values of ELISA assays for a) CHIKV viral antigen, b) CHIKV IgM, c) CHIKV IgG in CSF from patients with confirmed (n = 9) and suspected CHIKV (n = 37) with neurological complications and patients from non-CHIKV group (n = 15). Data are means +/- SD. * P < 0.05, ** P < 0.01 relative to control non-CHIKV subjects.
Mentions: The CSF positivities for CHIKV antigen in cases of confirmed neuro-CHIKV and suspected patients were 100% (9/9) and 76% (28/37) respectively, while the positivity for patients in the Non-CHIKV group was 13% (02/15) (Table 1). The mean absorbance value of CHIKV antigen in CSF from the CHIKV and non CHIKV groups as determined by the ELISA method is shown in Figure 1A. The mean absorbance value of CHIKV antigen in the confirmed neuro-CHIKV patients was 0.84 ± 0.19 and for suspected Chikungunya patients was 0.64 ± 0.19 which were significantly higher than the non CHIKV group (0.36 ± 0.08; P < 0.001). There was a significant difference in the mean CHIKV antigen activity between the confirmed neuro-CHIKV patients (0.84 ± 0.23) and the suspected-CHIKV patients (0.59 ± 0.14; P < 0.05).

Bottom Line: Early and cost-effective diagnosis of these patients remains problematic despite many new advanced diagnostic methods.A reliable diagnostic test, which could be performed in any standard pathology laboratory, would help to obtain definitive early diagnosis of CHIKV patients with neurological complications.The sensitivity for detection of IgM 48% or IgG 63% was significantly lower than the antigen assay (80%).

View Article: PubMed Central - HTML - PubMed

Affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, 88/2, Bajaj Nagar, Nagpur 440010, India. hfd_ciims@rediffmail.com.

ABSTRACT

Background: During Chikungunya virus (CHIKV) epidemic in Nagpur, India, we identified some suspected Chikungunya patients with neurological complications. Early and cost-effective diagnosis of these patients remains problematic despite many new advanced diagnostic methods. A reliable diagnostic test, which could be performed in any standard pathology laboratory, would help to obtain definitive early diagnosis of CHIKV patients with neurological complications. In our laboratory, in-house ELISA protocol for viral antigen, immunoglobulin M (IgM) and IgG detection has been developed and assessed for the diagnosis of CHIKV patients with neurological complications.

Method: Cerebrospinal fluid samples of forty-six patients who developed neurological symptoms within two months of CHIKV infections along with control subjects were included in the study and were analyzed for the presence of antigens and of IgM and IgG using an ELISA protocol.

Results: The ELISA method for antigen detection yielded 80% sensitivity and 87% specificity for the diagnosis of CHIKV patients with neurological complications. The sensitivity for detection of IgM 48% or IgG 63% was significantly lower than the antigen assay (80%).

Conclusion: The detection of viral antigen in CSF of CHIKV patients with neurological complications by ELISA method gave a more reliable diagnosis than antibodies detection that can be used to develop an immunodiagnostic assay with increased sensitivity and specificity.

No MeSH data available.


Related in: MedlinePlus