Limits...
The expression of cytokines in the aqueous humor and serum during endotoxin-induced uveitis in C3H/HeN mice.

Xu Y, Chen W, Lu H, Hu X, Li S, Wang J, Zhao L - Mol. Vis. (2010)

Bottom Line: At 24 h post-injection, the concentrations of IFN-gamma and IL-10 in the aqueous humor reached peak levels, and were significantly different from the control group (p=0.022 and p=0.003).The concentrations of IFN-gamma in serum at 4 and 24 hours were significantly different from the control group (p=0.033 and p=0.032).The concentration of IL-10 in serum, at 24 h post-injection, was also found to be significantly different from the control group (p=0.003).

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

ABSTRACT

Purpose: The cytokines present in the aqueous humor and serum of C3H/HeN mice with endotoxin-induced acute anterior uveitis were analyzed, and the potential role of the cytokines in the pathogenesis of the disease was investigated.

Methods: One hundred and eighty C3H/HeN mice were divided into an experimental group (n=150) and a control group (n=30). The mice in the experimental group were footpad-injected with 200 microg Vibro cholerae endotoxin (classical biotype, serotype Ogawa). The mice were then executed 4 h, 8 h, 16 h, 24 h, and 48 h after the injection of endotoxin. Aqueous humor and peripheral blood samples were collected using a microinjector. Ten samples were pooled together for analysis and centrifuged at 705x g, at 4 degrees C, for 3 min. The supernatant was collected and stored at -80 degrees C. The concentrations of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-10 (IL-10), and interferon-gamma (IFN-gamma) in the samples were measured using a Cytometric Bead Array (CBA).

Results: Acute anterior uveitis was successfully induced in C3H/HeN mice. At roughly 16 h post-injection, the concentrations of both IL-1 and IL-6 reached peak levels, and were significantly different from the control group (p=0.001 and p=0.001, respectively). At 24 h post-injection, the concentrations of IFN-gamma and IL-10 in the aqueous humor reached peak levels, and were significantly different from the control group (p=0.022 and p=0.003). The concentrations of IFN-gamma in serum at 4 and 24 hours were significantly different from the control group (p=0.033 and p=0.032). The concentration of IL-10 in serum, at 24 h post-injection, was also found to be significantly different from the control group (p=0.003). The cytokine expression levels in the aqueous humor were consistent with what would be expected during the process of inflammation.

Conclusions: Both IL-1 and IL-6 appear to play an important role in acute anterior uveitis; furthermore, the severity of inflammation may be associated with the dynamic balance of IFN-gamma and IL-10. Our results suggest that the cytokine network might be a useful therapeutic target in the treatment of acute anterior uveitis.

Show MeSH

Related in: MedlinePlus

The expression of interferon-γ in the aqueous humor and serum at different time points (expressed as the mean μg/l±SD). Interferon-γ concentrations in the aqueous humor and serum were not significantly different at 8 h or 16 h in comparison to the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2927430&req=5

f7: The expression of interferon-γ in the aqueous humor and serum at different time points (expressed as the mean μg/l±SD). Interferon-γ concentrations in the aqueous humor and serum were not significantly different at 8 h or 16 h in comparison to the control group.

Mentions: The concentration of cytokines at different time points in the aqueous humor are shown in Table 2. The concentrations of cytokines at different time points in the serum are shown in Table 3. Concentration of cytokines of different experimental groups in the aqueous humor and serum are shown in Figure 3, Figure 4, Figure 5, Figure 6, and Figure 7.


The expression of cytokines in the aqueous humor and serum during endotoxin-induced uveitis in C3H/HeN mice.

Xu Y, Chen W, Lu H, Hu X, Li S, Wang J, Zhao L - Mol. Vis. (2010)

The expression of interferon-γ in the aqueous humor and serum at different time points (expressed as the mean μg/l±SD). Interferon-γ concentrations in the aqueous humor and serum were not significantly different at 8 h or 16 h in comparison to the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2927430&req=5

f7: The expression of interferon-γ in the aqueous humor and serum at different time points (expressed as the mean μg/l±SD). Interferon-γ concentrations in the aqueous humor and serum were not significantly different at 8 h or 16 h in comparison to the control group.
Mentions: The concentration of cytokines at different time points in the aqueous humor are shown in Table 2. The concentrations of cytokines at different time points in the serum are shown in Table 3. Concentration of cytokines of different experimental groups in the aqueous humor and serum are shown in Figure 3, Figure 4, Figure 5, Figure 6, and Figure 7.

Bottom Line: At 24 h post-injection, the concentrations of IFN-gamma and IL-10 in the aqueous humor reached peak levels, and were significantly different from the control group (p=0.022 and p=0.003).The concentrations of IFN-gamma in serum at 4 and 24 hours were significantly different from the control group (p=0.033 and p=0.032).The concentration of IL-10 in serum, at 24 h post-injection, was also found to be significantly different from the control group (p=0.003).

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

ABSTRACT

Purpose: The cytokines present in the aqueous humor and serum of C3H/HeN mice with endotoxin-induced acute anterior uveitis were analyzed, and the potential role of the cytokines in the pathogenesis of the disease was investigated.

Methods: One hundred and eighty C3H/HeN mice were divided into an experimental group (n=150) and a control group (n=30). The mice in the experimental group were footpad-injected with 200 microg Vibro cholerae endotoxin (classical biotype, serotype Ogawa). The mice were then executed 4 h, 8 h, 16 h, 24 h, and 48 h after the injection of endotoxin. Aqueous humor and peripheral blood samples were collected using a microinjector. Ten samples were pooled together for analysis and centrifuged at 705x g, at 4 degrees C, for 3 min. The supernatant was collected and stored at -80 degrees C. The concentrations of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-10 (IL-10), and interferon-gamma (IFN-gamma) in the samples were measured using a Cytometric Bead Array (CBA).

Results: Acute anterior uveitis was successfully induced in C3H/HeN mice. At roughly 16 h post-injection, the concentrations of both IL-1 and IL-6 reached peak levels, and were significantly different from the control group (p=0.001 and p=0.001, respectively). At 24 h post-injection, the concentrations of IFN-gamma and IL-10 in the aqueous humor reached peak levels, and were significantly different from the control group (p=0.022 and p=0.003). The concentrations of IFN-gamma in serum at 4 and 24 hours were significantly different from the control group (p=0.033 and p=0.032). The concentration of IL-10 in serum, at 24 h post-injection, was also found to be significantly different from the control group (p=0.003). The cytokine expression levels in the aqueous humor were consistent with what would be expected during the process of inflammation.

Conclusions: Both IL-1 and IL-6 appear to play an important role in acute anterior uveitis; furthermore, the severity of inflammation may be associated with the dynamic balance of IFN-gamma and IL-10. Our results suggest that the cytokine network might be a useful therapeutic target in the treatment of acute anterior uveitis.

Show MeSH
Related in: MedlinePlus